A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.17/729 |
Resumo: | Diabetes mellitus is an epidemic multisystemic chronic disease that frequently is complicated by complex wound infections. Innovative topical antimicrobial therapy agents are potentially useful for multimodal treatment of these infections. However, an appropriately standardized in vivo model is currently not available to facilitate the screening of these emerging products and their effect on wound healing. To develop such a model, we analyzed, tested, and modified published models of wound healing. We optimized various aspects of the model, including animal species, diabetes induction method, hair removal technique, splint and dressing methods, the control of unintentional bacterial infection, sampling methods for the evaluation of bacterial burden, and aspects of the microscopic and macroscopic assessment of wound healing, all while taking into consideration animal welfare and the '3Rs' principle. We thus developed a new wound infection model in rats that is optimized for testing topical antimicrobial therapy agents. This model accurately reproduces the pathophysiology of infected diabetic wound healing and includes the current standard treatment (that is, debridement). The numerous benefits of this model include the ready availability of necessary materials, simple techniques, high reproducibility, and practicality for experiments with large sample sizes. Furthermore, given its similarities to infected-wound healing and treatment in humans, our new model can serve as a valid alternative for applied research. |
id |
RCAP_c27bd8c88c8fe0933fc06f920ab35977 |
---|---|
oai_identifier_str |
oai:repositorio.chlc.min-saude.pt:10400.17/729 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial TherapiesTerapêutica TópicaAnimaisTerapêutica e DosagemComplicações dos DiabetesModelos Animais de DoençasRatosProjectos de PesquisaInfecção de FeridasDiabetes mellitus is an epidemic multisystemic chronic disease that frequently is complicated by complex wound infections. Innovative topical antimicrobial therapy agents are potentially useful for multimodal treatment of these infections. However, an appropriately standardized in vivo model is currently not available to facilitate the screening of these emerging products and their effect on wound healing. To develop such a model, we analyzed, tested, and modified published models of wound healing. We optimized various aspects of the model, including animal species, diabetes induction method, hair removal technique, splint and dressing methods, the control of unintentional bacterial infection, sampling methods for the evaluation of bacterial burden, and aspects of the microscopic and macroscopic assessment of wound healing, all while taking into consideration animal welfare and the '3Rs' principle. We thus developed a new wound infection model in rats that is optimized for testing topical antimicrobial therapy agents. This model accurately reproduces the pathophysiology of infected diabetic wound healing and includes the current standard treatment (that is, debridement). The numerous benefits of this model include the ready availability of necessary materials, simple techniques, high reproducibility, and practicality for experiments with large sample sizes. Furthermore, given its similarities to infected-wound healing and treatment in humans, our new model can serve as a valid alternative for applied research.American Association for Laboratory Animal ScienceRepositório do Centro Hospitalar Universitário de Lisboa Central, EPEMendes, JJLeandro, CBonaparte, DPinto, A2012-11-08T15:59:17Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.17/729engComp Med. 2012 Feb;62(1):37-48info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-10T09:27:50Zoai:repositorio.chlc.min-saude.pt:10400.17/729Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:18:26.874690Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
title |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
spellingShingle |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies Mendes, JJ Terapêutica Tópica Animais Terapêutica e Dosagem Complicações dos Diabetes Modelos Animais de Doenças Ratos Projectos de Pesquisa Infecção de Feridas |
title_short |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
title_full |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
title_fullStr |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
title_full_unstemmed |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
title_sort |
A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies |
author |
Mendes, JJ |
author_facet |
Mendes, JJ Leandro, C Bonaparte, D Pinto, A |
author_role |
author |
author2 |
Leandro, C Bonaparte, D Pinto, A |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório do Centro Hospitalar Universitário de Lisboa Central, EPE |
dc.contributor.author.fl_str_mv |
Mendes, JJ Leandro, C Bonaparte, D Pinto, A |
dc.subject.por.fl_str_mv |
Terapêutica Tópica Animais Terapêutica e Dosagem Complicações dos Diabetes Modelos Animais de Doenças Ratos Projectos de Pesquisa Infecção de Feridas |
topic |
Terapêutica Tópica Animais Terapêutica e Dosagem Complicações dos Diabetes Modelos Animais de Doenças Ratos Projectos de Pesquisa Infecção de Feridas |
description |
Diabetes mellitus is an epidemic multisystemic chronic disease that frequently is complicated by complex wound infections. Innovative topical antimicrobial therapy agents are potentially useful for multimodal treatment of these infections. However, an appropriately standardized in vivo model is currently not available to facilitate the screening of these emerging products and their effect on wound healing. To develop such a model, we analyzed, tested, and modified published models of wound healing. We optimized various aspects of the model, including animal species, diabetes induction method, hair removal technique, splint and dressing methods, the control of unintentional bacterial infection, sampling methods for the evaluation of bacterial burden, and aspects of the microscopic and macroscopic assessment of wound healing, all while taking into consideration animal welfare and the '3Rs' principle. We thus developed a new wound infection model in rats that is optimized for testing topical antimicrobial therapy agents. This model accurately reproduces the pathophysiology of infected diabetic wound healing and includes the current standard treatment (that is, debridement). The numerous benefits of this model include the ready availability of necessary materials, simple techniques, high reproducibility, and practicality for experiments with large sample sizes. Furthermore, given its similarities to infected-wound healing and treatment in humans, our new model can serve as a valid alternative for applied research. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-08T15:59:17Z 2012 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.17/729 |
url |
http://hdl.handle.net/10400.17/729 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comp Med. 2012 Feb;62(1):37-48 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Association for Laboratory Animal Science |
publisher.none.fl_str_mv |
American Association for Laboratory Animal Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131281169055744 |