Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/3859 |
Resumo: | Avian tibial dyschondroplasia (TD) is a skeletal disease characterized by disruption of endochondral bone formation. The aim of this study was to determine the expression of extracellular matrix (ECM) macromolecules and ECM-degrading enzymes [matrix metalloproteinases (MMPs)] in the growth plates of normal and TD-affected 3-week-old broiler chicks (Cobb strain). Protein levels were analyzed by immunoblotting and gelatin zymography and gene expression by polymerase chain reaction. Expression of genes encoding the ECM macromolecules (collagen types II, IX, X and XI; and aggrecan) was not altered in dyschondroplasia; however, there was down-regulation of genes encoding MMP-9, MMP-13, MMP-10 and MMP-11 in addition to reduced amounts of MMP-2 and MMP-13 proteins. In contrast, there was up-regulation of genes encoding MMP-7 and the vascular endothelial growth factor. These findings suggest that the accumulation of cartilage associated with the disease may be the result of decreased proteolysis due to the down-regulation of MMPs and not to an increased production of ECM macromolecules. |
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Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasiaextracellular matrixgene expressiongrowth platematrix metalloproteinasestibial dyschondroplasiaAvian tibial dyschondroplasia (TD) is a skeletal disease characterized by disruption of endochondral bone formation. The aim of this study was to determine the expression of extracellular matrix (ECM) macromolecules and ECM-degrading enzymes [matrix metalloproteinases (MMPs)] in the growth plates of normal and TD-affected 3-week-old broiler chicks (Cobb strain). Protein levels were analyzed by immunoblotting and gelatin zymography and gene expression by polymerase chain reaction. Expression of genes encoding the ECM macromolecules (collagen types II, IX, X and XI; and aggrecan) was not altered in dyschondroplasia; however, there was down-regulation of genes encoding MMP-9, MMP-13, MMP-10 and MMP-11 in addition to reduced amounts of MMP-2 and MMP-13 proteins. In contrast, there was up-regulation of genes encoding MMP-7 and the vascular endothelial growth factor. These findings suggest that the accumulation of cartilage associated with the disease may be the result of decreased proteolysis due to the down-regulation of MMPs and not to an increased production of ECM macromolecules.ELSEVIER2012-01-19T13:05:23Z2012-01-192011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/3859http://hdl.handle.net/10174/3859porVelada I, Capela-Silva F, Reis F, Pires E, Egas C, Rodrigues-Santos P, Barros MT. Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia. Journal of Comparative Pathology 2011; 145: 174-186.174-186http://www.sciencedirect.com/science/article/pii/S002199751000366X145Journal of Comparative PathologyBIOivelada@uevora.ptfcs@uevora.ptndndndndnd226Velada, ICapela e Silva, FReis, FPires, EEgas, CRodrigues-Santos, PBarros, MTinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:23Zoai:dspace.uevora.pt:10174/3859Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:15.314657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
title |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
spellingShingle |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia Velada, I extracellular matrix gene expression growth plate matrix metalloproteinases tibial dyschondroplasia |
title_short |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
title_full |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
title_fullStr |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
title_full_unstemmed |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
title_sort |
Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia |
author |
Velada, I |
author_facet |
Velada, I Capela e Silva, F Reis, F Pires, E Egas, C Rodrigues-Santos, P Barros, MT |
author_role |
author |
author2 |
Capela e Silva, F Reis, F Pires, E Egas, C Rodrigues-Santos, P Barros, MT |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Velada, I Capela e Silva, F Reis, F Pires, E Egas, C Rodrigues-Santos, P Barros, MT |
dc.subject.por.fl_str_mv |
extracellular matrix gene expression growth plate matrix metalloproteinases tibial dyschondroplasia |
topic |
extracellular matrix gene expression growth plate matrix metalloproteinases tibial dyschondroplasia |
description |
Avian tibial dyschondroplasia (TD) is a skeletal disease characterized by disruption of endochondral bone formation. The aim of this study was to determine the expression of extracellular matrix (ECM) macromolecules and ECM-degrading enzymes [matrix metalloproteinases (MMPs)] in the growth plates of normal and TD-affected 3-week-old broiler chicks (Cobb strain). Protein levels were analyzed by immunoblotting and gelatin zymography and gene expression by polymerase chain reaction. Expression of genes encoding the ECM macromolecules (collagen types II, IX, X and XI; and aggrecan) was not altered in dyschondroplasia; however, there was down-regulation of genes encoding MMP-9, MMP-13, MMP-10 and MMP-11 in addition to reduced amounts of MMP-2 and MMP-13 proteins. In contrast, there was up-regulation of genes encoding MMP-7 and the vascular endothelial growth factor. These findings suggest that the accumulation of cartilage associated with the disease may be the result of decreased proteolysis due to the down-regulation of MMPs and not to an increased production of ECM macromolecules. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01T00:00:00Z 2012-01-19T13:05:23Z 2012-01-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/3859 http://hdl.handle.net/10174/3859 |
url |
http://hdl.handle.net/10174/3859 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Velada I, Capela-Silva F, Reis F, Pires E, Egas C, Rodrigues-Santos P, Barros MT. Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia. Journal of Comparative Pathology 2011; 145: 174-186. 174-186 http://www.sciencedirect.com/science/article/pii/S002199751000366X 145 Journal of Comparative Pathology BIO ivelada@uevora.pt fcs@uevora.pt nd nd nd nd nd 226 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
ELSEVIER |
publisher.none.fl_str_mv |
ELSEVIER |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136475308097536 |