Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/28370 |
Resumo: | Iron deficiency chlorosis (IDC) is an abiotic stress often experienced by soybean, owing to the low solubility of iron in alkaline soils. Here, soybean lines with contrasting Fe efficiencies were analyzed to test the hypothesis that the Fe efficiency trait is linked to antioxidative stress signaling via proper management of tissue Fe accumulation and transport, which in turn influences the regulation of heme and non heme containing enzymes involved in Fe uptake and ROS scavenging. Inefficient plants displayed higher oxidative stress and lower ferric reductase activity, whereas root and leaf catalase activity were nine-fold and three-fold higher, respectively. Efficient plants do not activate their antioxidant system because there is no formation of ROS under iron deficiency; while inefficient plants are not able to deal with ROS produced under iron deficiency because ascorbate peroxidase and superoxide dismutase are not activated because of the lack of iron as a cofactor, and of heme as a constituent of those enzymes. Superoxide dismutase and peroxidase isoenzymatic regulation may play a determinant role: 10 superoxide dismutase isoenzymes were observed in both cultivars, but iron superoxide dismutase activity was only detected in efficient plants; 15 peroxidase isoenzymes were observed in the roots and trifoliate leaves of efficient and inefficient cultivars and peroxidase activity levels were only increased in roots of efficient plants. |
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Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis∂-aminolevulinic acidFeSODHeme-containing enzymesHeminOxidative stressIron deficiency chlorosis (IDC) is an abiotic stress often experienced by soybean, owing to the low solubility of iron in alkaline soils. Here, soybean lines with contrasting Fe efficiencies were analyzed to test the hypothesis that the Fe efficiency trait is linked to antioxidative stress signaling via proper management of tissue Fe accumulation and transport, which in turn influences the regulation of heme and non heme containing enzymes involved in Fe uptake and ROS scavenging. Inefficient plants displayed higher oxidative stress and lower ferric reductase activity, whereas root and leaf catalase activity were nine-fold and three-fold higher, respectively. Efficient plants do not activate their antioxidant system because there is no formation of ROS under iron deficiency; while inefficient plants are not able to deal with ROS produced under iron deficiency because ascorbate peroxidase and superoxide dismutase are not activated because of the lack of iron as a cofactor, and of heme as a constituent of those enzymes. Superoxide dismutase and peroxidase isoenzymatic regulation may play a determinant role: 10 superoxide dismutase isoenzymes were observed in both cultivars, but iron superoxide dismutase activity was only detected in efficient plants; 15 peroxidase isoenzymes were observed in the roots and trifoliate leaves of efficient and inefficient cultivars and peroxidase activity levels were only increased in roots of efficient plants.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaSantos, Carla S.Ozgur, RenginUzilday, BarisTurkan, IsmailRoriz, MarianaRangel, António O. S. S.Carvalho, Susana M. P.Vasconcelos, Marta W.2019-10-15T18:34:35Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/28370engSantos, C. S., Ozgur, R., Uzilday, B., Turkan, I., Roriz, M., Rangel, A. O. S. S., … Vasconcelos, M. W. (2019). Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis. Plants, 8(9), 348. https://doi.org/10.3390/plants809034810.3390/plants80903482223-7747WOS:000487958100016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:33:59ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
title |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
spellingShingle |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis Santos, Carla S. ∂-aminolevulinic acid FeSOD Heme-containing enzymes Hemin Oxidative stress |
title_short |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
title_full |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
title_fullStr |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
title_full_unstemmed |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
title_sort |
Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis |
author |
Santos, Carla S. |
author_facet |
Santos, Carla S. Ozgur, Rengin Uzilday, Baris Turkan, Ismail Roriz, Mariana Rangel, António O. S. S. Carvalho, Susana M. P. Vasconcelos, Marta W. |
author_role |
author |
author2 |
Ozgur, Rengin Uzilday, Baris Turkan, Ismail Roriz, Mariana Rangel, António O. S. S. Carvalho, Susana M. P. Vasconcelos, Marta W. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Santos, Carla S. Ozgur, Rengin Uzilday, Baris Turkan, Ismail Roriz, Mariana Rangel, António O. S. S. Carvalho, Susana M. P. Vasconcelos, Marta W. |
dc.subject.por.fl_str_mv |
∂-aminolevulinic acid FeSOD Heme-containing enzymes Hemin Oxidative stress |
topic |
∂-aminolevulinic acid FeSOD Heme-containing enzymes Hemin Oxidative stress |
description |
Iron deficiency chlorosis (IDC) is an abiotic stress often experienced by soybean, owing to the low solubility of iron in alkaline soils. Here, soybean lines with contrasting Fe efficiencies were analyzed to test the hypothesis that the Fe efficiency trait is linked to antioxidative stress signaling via proper management of tissue Fe accumulation and transport, which in turn influences the regulation of heme and non heme containing enzymes involved in Fe uptake and ROS scavenging. Inefficient plants displayed higher oxidative stress and lower ferric reductase activity, whereas root and leaf catalase activity were nine-fold and three-fold higher, respectively. Efficient plants do not activate their antioxidant system because there is no formation of ROS under iron deficiency; while inefficient plants are not able to deal with ROS produced under iron deficiency because ascorbate peroxidase and superoxide dismutase are not activated because of the lack of iron as a cofactor, and of heme as a constituent of those enzymes. Superoxide dismutase and peroxidase isoenzymatic regulation may play a determinant role: 10 superoxide dismutase isoenzymes were observed in both cultivars, but iron superoxide dismutase activity was only detected in efficient plants; 15 peroxidase isoenzymes were observed in the roots and trifoliate leaves of efficient and inefficient cultivars and peroxidase activity levels were only increased in roots of efficient plants. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-15T18:34:35Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/28370 |
url |
http://hdl.handle.net/10400.14/28370 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, C. S., Ozgur, R., Uzilday, B., Turkan, I., Roriz, M., Rangel, A. O. S. S., … Vasconcelos, M. W. (2019). Understanding the role of the antioxidant system and the tetrapyrrole cycle in iron deficiency chlorosis. Plants, 8(9), 348. https://doi.org/10.3390/plants8090348 10.3390/plants8090348 2223-7747 WOS:000487958100016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303182188216320 |