Interaction between Covalent DNA Gels and a Cationic Surfactant
Main Author: | |
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Publication Date: | 2006 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10316/10374 https://doi.org/10.1021/bm0508919 |
Summary: | The interaction of covalently cross-linked double-stranded (ds) DNA gels and cetyltrimethylammonium bromide (CTAB) is investigated. The volume transition of the gels that follows the absorption of the oppositely charged surfactant from aqueous solution is studied. As do other polyelectrolyte networks, DNA networks form complexes with oppositely charged surfactant micelles at surfactant concentrations far below the critical micelle concentration (cmc) of the polymer-free solution. The size of the absorbed surfactant aggregates is determined from time-resolved fluorescence quenching (TRFQ). At low surfactant concentrations, small discrete micelles (160 < N < 210) are found, whereas large micelles (N > 500) form at surfactant concentrations of 1 mM. When the DNA is in excess of the surfactant, the surfactant binding is essentially quantitative. The gel volume decreases by 90% when the surfactant to DNA charge ratio, β, increases from 0 to 1. |
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Interaction between Covalent DNA Gels and a Cationic SurfactantThe interaction of covalently cross-linked double-stranded (ds) DNA gels and cetyltrimethylammonium bromide (CTAB) is investigated. The volume transition of the gels that follows the absorption of the oppositely charged surfactant from aqueous solution is studied. As do other polyelectrolyte networks, DNA networks form complexes with oppositely charged surfactant micelles at surfactant concentrations far below the critical micelle concentration (cmc) of the polymer-free solution. The size of the absorbed surfactant aggregates is determined from time-resolved fluorescence quenching (TRFQ). At low surfactant concentrations, small discrete micelles (160 < N < 210) are found, whereas large micelles (N > 500) form at surfactant concentrations of 1 mM. When the DNA is in excess of the surfactant, the surfactant binding is essentially quantitative. The gel volume decreases by 90% when the surfactant to DNA charge ratio, β, increases from 0 to 1.American Chemical Society2006-04-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10374http://hdl.handle.net/10316/10374https://doi.org/10.1021/bm0508919engBiomacromolecules. 7:4 (2006) 1090-10951525-7797Costa, DianaHansson, PerSchneider, StefanieMiguel, M. GraçaLindman, Björninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-02-11T18:17:32Zoai:estudogeral.uc.pt:10316/10374Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:14.871370Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
title |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
spellingShingle |
Interaction between Covalent DNA Gels and a Cationic Surfactant Costa, Diana |
title_short |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
title_full |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
title_fullStr |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
title_full_unstemmed |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
title_sort |
Interaction between Covalent DNA Gels and a Cationic Surfactant |
author |
Costa, Diana |
author_facet |
Costa, Diana Hansson, Per Schneider, Stefanie Miguel, M. Graça Lindman, Björn |
author_role |
author |
author2 |
Hansson, Per Schneider, Stefanie Miguel, M. Graça Lindman, Björn |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Costa, Diana Hansson, Per Schneider, Stefanie Miguel, M. Graça Lindman, Björn |
description |
The interaction of covalently cross-linked double-stranded (ds) DNA gels and cetyltrimethylammonium bromide (CTAB) is investigated. The volume transition of the gels that follows the absorption of the oppositely charged surfactant from aqueous solution is studied. As do other polyelectrolyte networks, DNA networks form complexes with oppositely charged surfactant micelles at surfactant concentrations far below the critical micelle concentration (cmc) of the polymer-free solution. The size of the absorbed surfactant aggregates is determined from time-resolved fluorescence quenching (TRFQ). At low surfactant concentrations, small discrete micelles (160 < N < 210) are found, whereas large micelles (N > 500) form at surfactant concentrations of 1 mM. When the DNA is in excess of the surfactant, the surfactant binding is essentially quantitative. The gel volume decreases by 90% when the surfactant to DNA charge ratio, β, increases from 0 to 1. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-04-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10374 http://hdl.handle.net/10316/10374 https://doi.org/10.1021/bm0508919 |
url |
http://hdl.handle.net/10316/10374 https://doi.org/10.1021/bm0508919 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomacromolecules. 7:4 (2006) 1090-1095 1525-7797 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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