Pollen Proteases Play Multiple Roles in Allergic Disorders
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/106210 https://doi.org/10.3390/ijms21103578 |
Resumo: | Allergic diseases are a major health concern worldwide. Pollens are important triggers for allergic rhinitis, conjunctivitis and asthma. Proteases released upon pollen grain hydration appear to play a major role in the typical immunological and inflammatory responses that occur in patients with allergic disorders. In this study, we aimed to identify specific proteolytic activity in a set of pollens with diverse allergenic potential. Diffusates from Chenopodium album, Plantago lanceolata and Eucalyptus globulus were added to a confluent monolayer of Calu-3 cells grown in an air-liquid interface system. We identified serine proteases and metalloproteinases in all pollen diffusates investigated. Proteases found in these pollen diffusates were shown to compromise the integrity of the lung epithelial barrier by disrupting transmembrane adhesion proteins E-cadherin, claudin-1 and Occludin, as well as, the cytosolic complex zonula occludens-1 (ZO-1) resulting in a time-dependent increase in transepithelial permeability. Tight junction disruption and increased transepithelial permeability facilitates allergen exposure to epithelial sub-layers contributing to the sensitization to a wide range of allergens. These pollen extracts also induced an increase in the release of interleukin 6 (IL-6) and interleukin 8 (IL-8) cytokines measured by flow cytometry possibly as a result of the activation of protease-activated receptors 2 (PAR-2). |
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Pollen Proteases Play Multiple Roles in Allergic Disordersallergypollen proteasestransepithelial permeabilityIL-6IL-8 and PAR-2Cell LineChenopodiumEucalyptusHumansHypersensitivityInterleukin-6Interleukin-8Peptide HydrolasesPlantagoPollenReceptor, PAR-2WaterAllergic diseases are a major health concern worldwide. Pollens are important triggers for allergic rhinitis, conjunctivitis and asthma. Proteases released upon pollen grain hydration appear to play a major role in the typical immunological and inflammatory responses that occur in patients with allergic disorders. In this study, we aimed to identify specific proteolytic activity in a set of pollens with diverse allergenic potential. Diffusates from Chenopodium album, Plantago lanceolata and Eucalyptus globulus were added to a confluent monolayer of Calu-3 cells grown in an air-liquid interface system. We identified serine proteases and metalloproteinases in all pollen diffusates investigated. Proteases found in these pollen diffusates were shown to compromise the integrity of the lung epithelial barrier by disrupting transmembrane adhesion proteins E-cadherin, claudin-1 and Occludin, as well as, the cytosolic complex zonula occludens-1 (ZO-1) resulting in a time-dependent increase in transepithelial permeability. Tight junction disruption and increased transepithelial permeability facilitates allergen exposure to epithelial sub-layers contributing to the sensitization to a wide range of allergens. These pollen extracts also induced an increase in the release of interleukin 6 (IL-6) and interleukin 8 (IL-8) cytokines measured by flow cytometry possibly as a result of the activation of protease-activated receptors 2 (PAR-2).MDPI2020-05-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106210http://hdl.handle.net/10316/106210https://doi.org/10.3390/ijms21103578eng1422-0067Gaspar, RicardoMatos, Mafalda Ramos deCortes, LuísaNunes-Correia, IsabelTodo-Bom, AnaPires, EuclidesVeríssimo, Paulainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-24T21:34:00Zoai:estudogeral.uc.pt:10316/106210Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:22:42.014556Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
title |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
spellingShingle |
Pollen Proteases Play Multiple Roles in Allergic Disorders Gaspar, Ricardo allergy pollen proteases transepithelial permeability IL-6 IL-8 and PAR-2 Cell Line Chenopodium Eucalyptus Humans Hypersensitivity Interleukin-6 Interleukin-8 Peptide Hydrolases Plantago Pollen Receptor, PAR-2 Water |
title_short |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
title_full |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
title_fullStr |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
title_full_unstemmed |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
title_sort |
Pollen Proteases Play Multiple Roles in Allergic Disorders |
author |
Gaspar, Ricardo |
author_facet |
Gaspar, Ricardo Matos, Mafalda Ramos de Cortes, Luísa Nunes-Correia, Isabel Todo-Bom, Ana Pires, Euclides Veríssimo, Paula |
author_role |
author |
author2 |
Matos, Mafalda Ramos de Cortes, Luísa Nunes-Correia, Isabel Todo-Bom, Ana Pires, Euclides Veríssimo, Paula |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gaspar, Ricardo Matos, Mafalda Ramos de Cortes, Luísa Nunes-Correia, Isabel Todo-Bom, Ana Pires, Euclides Veríssimo, Paula |
dc.subject.por.fl_str_mv |
allergy pollen proteases transepithelial permeability IL-6 IL-8 and PAR-2 Cell Line Chenopodium Eucalyptus Humans Hypersensitivity Interleukin-6 Interleukin-8 Peptide Hydrolases Plantago Pollen Receptor, PAR-2 Water |
topic |
allergy pollen proteases transepithelial permeability IL-6 IL-8 and PAR-2 Cell Line Chenopodium Eucalyptus Humans Hypersensitivity Interleukin-6 Interleukin-8 Peptide Hydrolases Plantago Pollen Receptor, PAR-2 Water |
description |
Allergic diseases are a major health concern worldwide. Pollens are important triggers for allergic rhinitis, conjunctivitis and asthma. Proteases released upon pollen grain hydration appear to play a major role in the typical immunological and inflammatory responses that occur in patients with allergic disorders. In this study, we aimed to identify specific proteolytic activity in a set of pollens with diverse allergenic potential. Diffusates from Chenopodium album, Plantago lanceolata and Eucalyptus globulus were added to a confluent monolayer of Calu-3 cells grown in an air-liquid interface system. We identified serine proteases and metalloproteinases in all pollen diffusates investigated. Proteases found in these pollen diffusates were shown to compromise the integrity of the lung epithelial barrier by disrupting transmembrane adhesion proteins E-cadherin, claudin-1 and Occludin, as well as, the cytosolic complex zonula occludens-1 (ZO-1) resulting in a time-dependent increase in transepithelial permeability. Tight junction disruption and increased transepithelial permeability facilitates allergen exposure to epithelial sub-layers contributing to the sensitization to a wide range of allergens. These pollen extracts also induced an increase in the release of interleukin 6 (IL-6) and interleukin 8 (IL-8) cytokines measured by flow cytometry possibly as a result of the activation of protease-activated receptors 2 (PAR-2). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/106210 http://hdl.handle.net/10316/106210 https://doi.org/10.3390/ijms21103578 |
url |
http://hdl.handle.net/10316/106210 https://doi.org/10.3390/ijms21103578 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1422-0067 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134115616784384 |