Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/9369 |
Resumo: | Oocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I. |
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7160 |
spelling |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodellingOocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I.Nature Publishing GroupSapientiaNavarro-Costa, PauloMcCarthy, AliciaPrudencio, PedroGreer, ChristinaGuilgur, Leonardo GastónBecker, Jorg D.Secombe, JulieRangan, PrashanthMartinho, Rui Goncalo2017-04-07T15:56:17Z2016-082016-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/9369eng2041-172310.1038/ncomms12331info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:20:48Zoai:sapientia.ualg.pt:10400.1/9369Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:01:20.041700Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
spellingShingle |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling Navarro-Costa, Paulo |
title_short |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_full |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_fullStr |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_full_unstemmed |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_sort |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
author |
Navarro-Costa, Paulo |
author_facet |
Navarro-Costa, Paulo McCarthy, Alicia Prudencio, Pedro Greer, Christina Guilgur, Leonardo Gastón Becker, Jorg D. Secombe, Julie Rangan, Prashanth Martinho, Rui Goncalo |
author_role |
author |
author2 |
McCarthy, Alicia Prudencio, Pedro Greer, Christina Guilgur, Leonardo Gastón Becker, Jorg D. Secombe, Julie Rangan, Prashanth Martinho, Rui Goncalo |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Navarro-Costa, Paulo McCarthy, Alicia Prudencio, Pedro Greer, Christina Guilgur, Leonardo Gastón Becker, Jorg D. Secombe, Julie Rangan, Prashanth Martinho, Rui Goncalo |
description |
Oocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08 2016-08-01T00:00:00Z 2017-04-07T15:56:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/9369 |
url |
http://hdl.handle.net/10400.1/9369 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2041-1723 10.1038/ncomms12331 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133242612252672 |