Automatic tracking of labeled red blood cells in microchannels
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/10355 |
Resumo: | The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland–Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels. |
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Automatic tracking of labeled red blood cells in microchannelsImage analysisAutomatic methodRed blood cellsIn vitro blood flowThe current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland–Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels.The authors acknowledge the financial support provided by: Student Mobility Placements with the program Lifelong Learning(Erasmus Program), PTDC/SAUBEB/108728/2008, PTDC/SAU-BEB/105650/2008, PTDC/EMEMFE/099109/2008 and PTDC/SAU-ENB/116929/2010 from the FCT (Science and Technology Foundation) and COMPETE, Portugal.John Wiley & SonsBiblioteca Digital do IPBPinho, DianaLima, Rui A.Pereira, Ana I.Gayubo, Fernando2014-09-08T14:17:44Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/10355engPinho, D.; Lima, R.; Pereira, Ana I.; Gayubo, F. (2013). Automatic tracking of labeled red blood cells in microchannels. International Journal for Numerical Methods in Biomedical Engineering. ISSN 2040-7947. 29, p. 977-98710.1002/cnm.2526info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:24:22Zoai:bibliotecadigital.ipb.pt:10198/10355Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:01:09.310789Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Automatic tracking of labeled red blood cells in microchannels |
title |
Automatic tracking of labeled red blood cells in microchannels |
spellingShingle |
Automatic tracking of labeled red blood cells in microchannels Pinho, Diana Image analysis Automatic method Red blood cells In vitro blood flow |
title_short |
Automatic tracking of labeled red blood cells in microchannels |
title_full |
Automatic tracking of labeled red blood cells in microchannels |
title_fullStr |
Automatic tracking of labeled red blood cells in microchannels |
title_full_unstemmed |
Automatic tracking of labeled red blood cells in microchannels |
title_sort |
Automatic tracking of labeled red blood cells in microchannels |
author |
Pinho, Diana |
author_facet |
Pinho, Diana Lima, Rui A. Pereira, Ana I. Gayubo, Fernando |
author_role |
author |
author2 |
Lima, Rui A. Pereira, Ana I. Gayubo, Fernando |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Pinho, Diana Lima, Rui A. Pereira, Ana I. Gayubo, Fernando |
dc.subject.por.fl_str_mv |
Image analysis Automatic method Red blood cells In vitro blood flow |
topic |
Image analysis Automatic method Red blood cells In vitro blood flow |
description |
The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland–Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z 2014-09-08T14:17:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/10355 |
url |
http://hdl.handle.net/10198/10355 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinho, D.; Lima, R.; Pereira, Ana I.; Gayubo, F. (2013). Automatic tracking of labeled red blood cells in microchannels. International Journal for Numerical Methods in Biomedical Engineering. ISSN 2040-7947. 29, p. 977-987 10.1002/cnm.2526 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley & Sons |
publisher.none.fl_str_mv |
John Wiley & Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135242848567296 |