NF-kB pathway controls mitochondrial dynamics
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/40084 |
Resumo: | The Optic atrophy 1 protein (OPA1) is a key element in the dynamics and morphology of mitochondria. We demonstrated that the absence of I?B kinase-a, which is a key element of the nonclassical NF-?B pathway, has an impact on the mitochondrial network morphology and OPA1 expression. In contrast, the absence of NF-?B essential modulator (NEMO) or I?B kinase-ß, both of which are essential for the canonical NF-?B pathway, has no impact on mitochondrial dynamics. Whereas Parkin has been reported to positively regulate the expression of OPA1 through NEMO, herein we found that PARK2 overexpression did not modify the expression of OPA1. PARK2 expression reduced the levels of Bax, and it prevented stress-induced cell death only in Bak-deficient mouse embryonic fibroblast cells. Collectively, our results point out a role of the nonclassical NF-?B pathway in the regulation of mitochondrial dynamics and OPA1 expression. |
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NF-kB pathway controls mitochondrial dynamicsScience & TechnologyThe Optic atrophy 1 protein (OPA1) is a key element in the dynamics and morphology of mitochondria. We demonstrated that the absence of I?B kinase-a, which is a key element of the nonclassical NF-?B pathway, has an impact on the mitochondrial network morphology and OPA1 expression. In contrast, the absence of NF-?B essential modulator (NEMO) or I?B kinase-ß, both of which are essential for the canonical NF-?B pathway, has no impact on mitochondrial dynamics. Whereas Parkin has been reported to positively regulate the expression of OPA1 through NEMO, herein we found that PARK2 overexpression did not modify the expression of OPA1. PARK2 expression reduced the levels of Bax, and it prevented stress-induced cell death only in Bak-deficient mouse embryonic fibroblast cells. Collectively, our results point out a role of the nonclassical NF-?B pathway in the regulation of mitochondrial dynamics and OPA1 expression.This work was funded by grants to JE from the Agence Nationale de Recherches sur le Sida et les Hepatites Virales (ANRS), the Association pour la Recherche sur le Cancer (ARC), La Ligue contre le Cancer (Ligue), and the Fondation pour la Recherche Medicale; OC is supported by the Fondation ICM, 'Investissements d'avenir' ANR-10-IAIHU-06. ML is supported by fellowships from the ANRS. VR is supported by a fellowship from FCT code SFRH/BD/64064/2009. We thank C Soundaramourty for her help, and V Baud who kindly provided the I kappa B kinase-alpha-deficient MEF cells IKK beta<SUP>-/-</SUP>, and IKK alpha beta<SUP>-/-</SUP>. MEF cells were provided by Inder M. Verma, and NEMO MEFs cells were a gift from Marc Schmidt-Supprian. JE acknowledges the support of the Canada Research Chair program.Nature Publishing GroupUniversidade do MinhoLaforge, M.Rodrigues, VascoSilvestre, Ricardo Jorge LealGautier, C.Weil, R.Corti, O.Estaquier, J.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40084engLaforge, M., Rodrigues, V., Silvestre, R., Gautier, C., Weil, R., Corti, O., & Estaquier, J. (2016). NF-kappa B pathway controls mitochondrial dynamics. Cell Death and Differentiation, 23(1), 89-98. doi: 10.1038/cdd.2015.422041-488910.1038/cdd.2015.4226024391http://www.nature.com/cddis/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:16:30Zoai:repositorium.sdum.uminho.pt:1822/40084Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:02.275401Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
NF-kB pathway controls mitochondrial dynamics |
title |
NF-kB pathway controls mitochondrial dynamics |
spellingShingle |
NF-kB pathway controls mitochondrial dynamics Laforge, M. Science & Technology |
title_short |
NF-kB pathway controls mitochondrial dynamics |
title_full |
NF-kB pathway controls mitochondrial dynamics |
title_fullStr |
NF-kB pathway controls mitochondrial dynamics |
title_full_unstemmed |
NF-kB pathway controls mitochondrial dynamics |
title_sort |
NF-kB pathway controls mitochondrial dynamics |
author |
Laforge, M. |
author_facet |
Laforge, M. Rodrigues, Vasco Silvestre, Ricardo Jorge Leal Gautier, C. Weil, R. Corti, O. Estaquier, J. |
author_role |
author |
author2 |
Rodrigues, Vasco Silvestre, Ricardo Jorge Leal Gautier, C. Weil, R. Corti, O. Estaquier, J. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Laforge, M. Rodrigues, Vasco Silvestre, Ricardo Jorge Leal Gautier, C. Weil, R. Corti, O. Estaquier, J. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
The Optic atrophy 1 protein (OPA1) is a key element in the dynamics and morphology of mitochondria. We demonstrated that the absence of I?B kinase-a, which is a key element of the nonclassical NF-?B pathway, has an impact on the mitochondrial network morphology and OPA1 expression. In contrast, the absence of NF-?B essential modulator (NEMO) or I?B kinase-ß, both of which are essential for the canonical NF-?B pathway, has no impact on mitochondrial dynamics. Whereas Parkin has been reported to positively regulate the expression of OPA1 through NEMO, herein we found that PARK2 overexpression did not modify the expression of OPA1. PARK2 expression reduced the levels of Bax, and it prevented stress-induced cell death only in Bak-deficient mouse embryonic fibroblast cells. Collectively, our results point out a role of the nonclassical NF-?B pathway in the regulation of mitochondrial dynamics and OPA1 expression. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/40084 |
url |
http://hdl.handle.net/1822/40084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Laforge, M., Rodrigues, V., Silvestre, R., Gautier, C., Weil, R., Corti, O., & Estaquier, J. (2016). NF-kappa B pathway controls mitochondrial dynamics. Cell Death and Differentiation, 23(1), 89-98. doi: 10.1038/cdd.2015.42 2041-4889 10.1038/cdd.2015.42 26024391 http://www.nature.com/cddis/index.html |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132515133292544 |