Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity

Detalhes bibliográficos
Autor(a) principal: Castelo-Branco, João Diogo Cristovão Pinto
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/2881
Resumo: Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
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spelling Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activitybroccoliBrassica oleracea var. italicamyrosinasewater activitytemperaturekineticsmirosinaseactividade da águatemperaturaMestrado em Engenharia Alimentar - Instituto Superior de AgronomiaEpidemiological studies have shown that a diet rich in Brassica vegetables is associated with a decreasing risk of incidence of some types of cancer. These health promoting effects are most likely due to the breakdown products of glucosinolates formed by the action of myrosinase which catalyzes the hydrolysis of glucosinolates, found in Brassica plants. Industrial processing of food can affect the content, composition, activity and bioavailability of glucosinolates breakdown products, due to cell lysis or glucosinolates and myrosinase degradation. The aim of this thesis is to investigate the combined effect of moisture content (water activity) and temperature on myrosinase stability. The study was conducted in batches of freeze dried broccoli with different moisture contents. These batches of broccoli were then heated at different time/temperature combinations and myrosinase activity was analysed, the obtained results from these measurements were kinetically modeled. Myrosinase was shown to be much less thermo stable in higher moisture content samples than in the driest samples. Thermal stability (30-80 ºC) of myrosinase was described by a first order kinetic model. Besides showing higher myrosinase thermal stability, driest samples show lower activation energies, i.e, lower increase of degradation rate when temperature increases.ISAMartins, Margarida MoldãoRepositório da Universidade de LisboaCastelo-Branco, João Diogo Cristovão Pinto2011-01-28T10:40:22Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/2881engCastelo-Branco, João Diogo Cristovão Pinto - Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity. Lisboa, Instituto Superior de Agronomia, 2010, 90 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:34:02Zoai:www.repository.utl.pt:10400.5/2881Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:50:51.215326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
title Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
spellingShingle Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
Castelo-Branco, João Diogo Cristovão Pinto
broccoli
Brassica oleracea var. italica
myrosinase
water activity
temperature
kinetics
mirosinase
actividade da água
temperatura
title_short Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
title_full Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
title_fullStr Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
title_full_unstemmed Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
title_sort Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity
author Castelo-Branco, João Diogo Cristovão Pinto
author_facet Castelo-Branco, João Diogo Cristovão Pinto
author_role author
dc.contributor.none.fl_str_mv Martins, Margarida Moldão
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Castelo-Branco, João Diogo Cristovão Pinto
dc.subject.por.fl_str_mv broccoli
Brassica oleracea var. italica
myrosinase
water activity
temperature
kinetics
mirosinase
actividade da água
temperatura
topic broccoli
Brassica oleracea var. italica
myrosinase
water activity
temperature
kinetics
mirosinase
actividade da água
temperatura
description Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2011-01-28T10:40:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/2881
url http://hdl.handle.net/10400.5/2881
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castelo-Branco, João Diogo Cristovão Pinto - Myrosinase stability in broccoli (Brassica oleracea var. italica). Influence of temperature and water activity. Lisboa, Instituto Superior de Agronomia, 2010, 90 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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