Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/23876 |
Resumo: | Energy metabolism in yeast cells can be manipulated by providing different carbon sources: Saccharomyces cerevisiae grown on glucose rapidly proliferates by means of fermentation, whereas in non-fermentable carbon sources, such as glycerol, metabolism shifts towards respiration. In general, fermentation is preferred by the yeast in rapidly proliferating cells even in the presence of oxygen, a process also called the Crabtree effect. In the present study, it was observed that the addition of glucose to S. cerevisiae UE-ME3 grown in a glycerol-rich medium caused an increase in the growth-marker enzyme alkaline phosphatase and a blockage of respiratory enzymes. In addition, the simultaneous exposure of yeast cells to TiO2-NP <100 nm (5 μg/mL) under heat shock (28/40), after 100 min of culture in respiratory-fermentative mode, caused a partial reversion of glucose-mediated repression of citrate synthase and succinate dehydrogenase activities, key-enzymes of the citrate cycle, and a decrease in the level of alkaline phosphatase activity similar to those detected in cells grown in respiratory mode. |
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Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cyclealkaline phosphatasepyruvate kinasecitrate synthasesuccinate dehydrogenasenanomaterialsEnergy metabolism in yeast cells can be manipulated by providing different carbon sources: Saccharomyces cerevisiae grown on glucose rapidly proliferates by means of fermentation, whereas in non-fermentable carbon sources, such as glycerol, metabolism shifts towards respiration. In general, fermentation is preferred by the yeast in rapidly proliferating cells even in the presence of oxygen, a process also called the Crabtree effect. In the present study, it was observed that the addition of glucose to S. cerevisiae UE-ME3 grown in a glycerol-rich medium caused an increase in the growth-marker enzyme alkaline phosphatase and a blockage of respiratory enzymes. In addition, the simultaneous exposure of yeast cells to TiO2-NP <100 nm (5 μg/mL) under heat shock (28/40), after 100 min of culture in respiratory-fermentative mode, caused a partial reversion of glucose-mediated repression of citrate synthase and succinate dehydrogenase activities, key-enzymes of the citrate cycle, and a decrease in the level of alkaline phosphatase activity similar to those detected in cells grown in respiratory mode.Formatex, Research Center2019-01-08T14:34:59Z2019-01-082018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/23876http://hdl.handle.net/10174/23876porCapela-Pires J, Ferreira R, Alves-Pereira I. (2018) Chapter title: "Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle" In: A. Méndez-Vilas (ed), Global progress in applied microbiology: a multidisciplinary approach. 1sd ed Formatex, Research Center, Badajpoz, Spain. pp: 79-83 (both included). ISBN-13: 978-84-947512-2-6978-84-947512-2-6jmcp@uevora.ptraf@uevora.ptiap@uevora.pt365Capela-Pires, JFerreira, RAlves-Pereira, Iinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:15:59Zoai:dspace.uevora.pt:10174/23876Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:14:28.099554Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
title |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
spellingShingle |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle Capela-Pires, J alkaline phosphatase pyruvate kinase citrate synthase succinate dehydrogenase nanomaterials |
title_short |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
title_full |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
title_fullStr |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
title_full_unstemmed |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
title_sort |
Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle |
author |
Capela-Pires, J |
author_facet |
Capela-Pires, J Ferreira, R Alves-Pereira, I |
author_role |
author |
author2 |
Ferreira, R Alves-Pereira, I |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Capela-Pires, J Ferreira, R Alves-Pereira, I |
dc.subject.por.fl_str_mv |
alkaline phosphatase pyruvate kinase citrate synthase succinate dehydrogenase nanomaterials |
topic |
alkaline phosphatase pyruvate kinase citrate synthase succinate dehydrogenase nanomaterials |
description |
Energy metabolism in yeast cells can be manipulated by providing different carbon sources: Saccharomyces cerevisiae grown on glucose rapidly proliferates by means of fermentation, whereas in non-fermentable carbon sources, such as glycerol, metabolism shifts towards respiration. In general, fermentation is preferred by the yeast in rapidly proliferating cells even in the presence of oxygen, a process also called the Crabtree effect. In the present study, it was observed that the addition of glucose to S. cerevisiae UE-ME3 grown in a glycerol-rich medium caused an increase in the growth-marker enzyme alkaline phosphatase and a blockage of respiratory enzymes. In addition, the simultaneous exposure of yeast cells to TiO2-NP <100 nm (5 μg/mL) under heat shock (28/40), after 100 min of culture in respiratory-fermentative mode, caused a partial reversion of glucose-mediated repression of citrate synthase and succinate dehydrogenase activities, key-enzymes of the citrate cycle, and a decrease in the level of alkaline phosphatase activity similar to those detected in cells grown in respiratory mode. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01T00:00:00Z 2019-01-08T14:34:59Z 2019-01-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/23876 http://hdl.handle.net/10174/23876 |
url |
http://hdl.handle.net/10174/23876 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Capela-Pires J, Ferreira R, Alves-Pereira I. (2018) Chapter title: "Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle" In: A. Méndez-Vilas (ed), Global progress in applied microbiology: a multidisciplinary approach. 1sd ed Formatex, Research Center, Badajpoz, Spain. pp: 79-83 (both included). ISBN-13: 978-84-947512-2-6 978-84-947512-2-6 jmcp@uevora.pt raf@uevora.pt iap@uevora.pt 365 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Formatex, Research Center |
publisher.none.fl_str_mv |
Formatex, Research Center |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136626019926016 |