Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers

Detalhes bibliográficos
Autor(a) principal: Souilem, Fedia
Data de Publicação: 2018
Outros Autores: Dias, Maria Inês, Barros, Lillian, Calhelha, Ricardo C., Alves, Maria José, Harzallah-Skhiri, Fethia, Ferreira, Isabel C.F.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/20086
Resumo: The present work aimed to characterize leaves, stems, and flowers of Carissa macrocarpa (Eckl.) A.DC., by performing an analysis of the phenolic compounds by HPLC-DAD/ESI-MS, correlating them with bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. Thirty polyphenols were identified in the hydroethanolic extract, including phenolic acids, flavan-3-ols, and flavonol glycosides derivatives (which presented the highest number of identified compounds). However, flavan-3-ols showed the highest concentration in stems (mainly owing to the presence of dimers, trimmers, and tetramers of type B (epi)catechin). Leaves were distinguished by their high antioxidant and anti-inflammatory activities, as well as their bactericidal e ect against E. coli, while stems presented a higher cytotoxic activity and bactericidal e ect against Gram-positive bacteria. Moreover, a high correlation between the studied bioactivities and the presence of phenolic compounds was also verified. The obtained results bring added value to the studied plant species.
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spelling Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowersBioactivitiesCarissa macrocarpaPearson's correlationPhenolic profilePlant aerial partsThe present work aimed to characterize leaves, stems, and flowers of Carissa macrocarpa (Eckl.) A.DC., by performing an analysis of the phenolic compounds by HPLC-DAD/ESI-MS, correlating them with bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. Thirty polyphenols were identified in the hydroethanolic extract, including phenolic acids, flavan-3-ols, and flavonol glycosides derivatives (which presented the highest number of identified compounds). However, flavan-3-ols showed the highest concentration in stems (mainly owing to the presence of dimers, trimmers, and tetramers of type B (epi)catechin). Leaves were distinguished by their high antioxidant and anti-inflammatory activities, as well as their bactericidal e ect against E. coli, while stems presented a higher cytotoxic activity and bactericidal e ect against Gram-positive bacteria. Moreover, a high correlation between the studied bioactivities and the presence of phenolic compounds was also verified. The obtained results bring added value to the studied plant species.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programmer PT2020 for financial support to CIMO (UID/AGR/00690/2019); L. Barros and M.I. Dias thank the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts. The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBSouilem, FediaDias, Maria InêsBarros, LillianCalhelha, Ricardo C.Alves, Maria JoséHarzallah-Skhiri, FethiaFerreira, Isabel C.F.R.2018-01-19T10:00:00Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/20086engSouilem, Fedia; Dias, Maria Inês; Barros, Lillian; Calhelha, Ricardo C.; Alves, Maria José; Harzallah-Skhiri, Fethia; Ferreira, Isabel C.F.R. (2019). Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers. Molecules. ISSN 1420-3049. 24, p. 1-1210.3390/molecules24091696info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:45:54Zoai:bibliotecadigital.ipb.pt:10198/20086Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:10:52.918550Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
title Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
spellingShingle Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
Souilem, Fedia
Bioactivities
Carissa macrocarpa
Pearson's correlation
Phenolic profile
Plant aerial parts
title_short Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
title_full Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
title_fullStr Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
title_full_unstemmed Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
title_sort Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
author Souilem, Fedia
author_facet Souilem, Fedia
Dias, Maria Inês
Barros, Lillian
Calhelha, Ricardo C.
Alves, Maria José
Harzallah-Skhiri, Fethia
Ferreira, Isabel C.F.R.
author_role author
author2 Dias, Maria Inês
Barros, Lillian
Calhelha, Ricardo C.
Alves, Maria José
Harzallah-Skhiri, Fethia
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Souilem, Fedia
Dias, Maria Inês
Barros, Lillian
Calhelha, Ricardo C.
Alves, Maria José
Harzallah-Skhiri, Fethia
Ferreira, Isabel C.F.R.
dc.subject.por.fl_str_mv Bioactivities
Carissa macrocarpa
Pearson's correlation
Phenolic profile
Plant aerial parts
topic Bioactivities
Carissa macrocarpa
Pearson's correlation
Phenolic profile
Plant aerial parts
description The present work aimed to characterize leaves, stems, and flowers of Carissa macrocarpa (Eckl.) A.DC., by performing an analysis of the phenolic compounds by HPLC-DAD/ESI-MS, correlating them with bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. Thirty polyphenols were identified in the hydroethanolic extract, including phenolic acids, flavan-3-ols, and flavonol glycosides derivatives (which presented the highest number of identified compounds). However, flavan-3-ols showed the highest concentration in stems (mainly owing to the presence of dimers, trimmers, and tetramers of type B (epi)catechin). Leaves were distinguished by their high antioxidant and anti-inflammatory activities, as well as their bactericidal e ect against E. coli, while stems presented a higher cytotoxic activity and bactericidal e ect against Gram-positive bacteria. Moreover, a high correlation between the studied bioactivities and the presence of phenolic compounds was also verified. The obtained results bring added value to the studied plant species.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-19T10:00:00Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/20086
url http://hdl.handle.net/10198/20086
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Souilem, Fedia; Dias, Maria Inês; Barros, Lillian; Calhelha, Ricardo C.; Alves, Maria José; Harzallah-Skhiri, Fethia; Ferreira, Isabel C.F.R. (2019). Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers. Molecules. ISSN 1420-3049. 24, p. 1-12
10.3390/molecules24091696
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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