Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications

Detalhes bibliográficos
Autor(a) principal: Carvalho, A. M.
Data de Publicação: 2018
Outros Autores: Marques, A. P., Silva, Tiago José Quinteiros Lopes Henriques, Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/58769
Resumo: Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications.
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spelling Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applicationsASTM GuidelinesBiologic activityBiomedical applicationBiophysical characterizationCodfishMarine biomaterialsMarine-origin collagenCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Biotecnologia IndustrialScience & TechnologyCollagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications.European Research Council grant agreement ERC-2012-ADG 20120216-321266 for the project ComplexiTEinfo:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoCarvalho, A. M.Marques, A. P.Silva, Tiago José Quinteiros Lopes HenriquesReis, R. L.2018-122018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58769engCarvalho A. M., Marques A. P., Silva T. H., Reis R. L. Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications, Marine Drugs, Vol. 16, Issue 12, pp. 495-509, doi:10.3390/md16120495, 20181660-339710.3390/md1612049530544788https://www.mdpi.com/1660-3397/16/12/495info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:23:18ZPortal AgregadorONG
dc.title.none.fl_str_mv Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
title Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
spellingShingle Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
Carvalho, A. M.
ASTM Guidelines
Biologic activity
Biomedical application
Biophysical characterization
Codfish
Marine biomaterials
Marine-origin collagen
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
title_short Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
title_full Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
title_fullStr Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
title_full_unstemmed Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
title_sort Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
author Carvalho, A. M.
author_facet Carvalho, A. M.
Marques, A. P.
Silva, Tiago José Quinteiros Lopes Henriques
Reis, R. L.
author_role author
author2 Marques, A. P.
Silva, Tiago José Quinteiros Lopes Henriques
Reis, R. L.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Carvalho, A. M.
Marques, A. P.
Silva, Tiago José Quinteiros Lopes Henriques
Reis, R. L.
dc.subject.por.fl_str_mv ASTM Guidelines
Biologic activity
Biomedical application
Biophysical characterization
Codfish
Marine biomaterials
Marine-origin collagen
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
topic ASTM Guidelines
Biologic activity
Biomedical application
Biophysical characterization
Codfish
Marine biomaterials
Marine-origin collagen
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
description Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
2018-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/58769
url http://hdl.handle.net/1822/58769
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carvalho A. M., Marques A. P., Silva T. H., Reis R. L. Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications, Marine Drugs, Vol. 16, Issue 12, pp. 495-509, doi:10.3390/md16120495, 2018
1660-3397
10.3390/md16120495
30544788
https://www.mdpi.com/1660-3397/16/12/495
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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