Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach

Detalhes bibliográficos
Autor(a) principal: Janek, Tomasz
Data de Publicação: 2019
Outros Autores: Czelen, Przemyslaw, Gudiña, Eduardo José, Rodrigues, L. R., Czyznikowska, Zaneta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/58393
Resumo: The molecular interactions between two single-chain lysosomotropic surfactants DMM-11 (2-Dodecanoyloxyethyl)trimethylammonium bromide) and DMPM-11 (2-Dodecanoyloxypropyl)trimethylammonium bromide) with a small heme-protein (cytochrome c (cyt-c)) in Hepes buffer (pH = 7.4) were extensively investigated by surface tension, dynamic light scattering (DLS), circular dichroism (CD) and fluorescence spectroscopy in combination with molecular dynamic simulation techniques. The results demonstrated that surfactants can destroy the hydrophobic cavity of cyt-c, make the α-helical become loose and convert it into the β-sheet structure. The interactions between surfactants and cyt-c are mainly hydrophobic. Molecular modelling approaches were also used to gather a deeper insight on the binding of lysosomotropic surfactants with cyt-c and the in silico results were found to be in good agreement with the experimental ones. This study provides a molecular basis for the applications of protein-surfactant complexes in biological, food, pharmaceutical, industrial and cosmetic systems
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spelling Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approachLysosomotropic surfactantCytochromeFluorescence quenchingCircular dichroismMolecular dynamic simulationsCytochrome cEngenharia e Tecnologia::Biotecnologia IndustrialScience & TechnologyThe molecular interactions between two single-chain lysosomotropic surfactants DMM-11 (2-Dodecanoyloxyethyl)trimethylammonium bromide) and DMPM-11 (2-Dodecanoyloxypropyl)trimethylammonium bromide) with a small heme-protein (cytochrome c (cyt-c)) in Hepes buffer (pH = 7.4) were extensively investigated by surface tension, dynamic light scattering (DLS), circular dichroism (CD) and fluorescence spectroscopy in combination with molecular dynamic simulation techniques. The results demonstrated that surfactants can destroy the hydrophobic cavity of cyt-c, make the α-helical become loose and convert it into the β-sheet structure. The interactions between surfactants and cyt-c are mainly hydrophobic. Molecular modelling approaches were also used to gather a deeper insight on the binding of lysosomotropic surfactants with cyt-c and the in silico results were found to be in good agreement with the experimental ones. This study provides a molecular basis for the applications of protein-surfactant complexes in biological, food, pharmaceutical, industrial and cosmetic systemsPolish-Portugal Executive Program for years 2017–2018 sponsored by the Polish Ministry of Science and Higher Education and by the Portuguese Foundation for Science and Technology (FCT). Additionally, FCT supported the study under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684). T. Janek was supported by the National Science Centre, Poland, projects 2017/26/E/NZ9/00975 and 2018/02/X/NZ6/02201. L. R. Rodrigues acknowledges FCT for the grant SFRH/BSAB/142873/2018. E. J. Gudiña was supported by the grant UMINHO/BPD/39/2015 funded by FCT under the scope of the project UID/BIO/04469/2013info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoJanek, TomaszCzelen, PrzemyslawGudiña, Eduardo JoséRodrigues, L. R.Czyznikowska, Zaneta2019-042019-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58393engJanek, Tomasz; Czelen, Przemyslaw; Gudiña, Eduardo J.; Rodrigues, Lígia R.; Czyznikowska, Zaneta, Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach. International Journal of Biological Macromolecules, 126, 1177-1185, 20190141-81301879-000310.1016/j.ijbiomac.2019.01.02430625357https://www.sciencedirect.com/science/article/pii/S0141813018365012info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:06Zoai:repositorium.sdum.uminho.pt:1822/58393Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:52:24.612947Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
title Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
spellingShingle Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
Janek, Tomasz
Lysosomotropic surfactant
Cytochrome
Fluorescence quenching
Circular dichroism
Molecular dynamic simulations
Cytochrome c
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
title_short Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
title_full Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
title_fullStr Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
title_full_unstemmed Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
title_sort Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
author Janek, Tomasz
author_facet Janek, Tomasz
Czelen, Przemyslaw
Gudiña, Eduardo José
Rodrigues, L. R.
Czyznikowska, Zaneta
author_role author
author2 Czelen, Przemyslaw
Gudiña, Eduardo José
Rodrigues, L. R.
Czyznikowska, Zaneta
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Janek, Tomasz
Czelen, Przemyslaw
Gudiña, Eduardo José
Rodrigues, L. R.
Czyznikowska, Zaneta
dc.subject.por.fl_str_mv Lysosomotropic surfactant
Cytochrome
Fluorescence quenching
Circular dichroism
Molecular dynamic simulations
Cytochrome c
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
topic Lysosomotropic surfactant
Cytochrome
Fluorescence quenching
Circular dichroism
Molecular dynamic simulations
Cytochrome c
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
description The molecular interactions between two single-chain lysosomotropic surfactants DMM-11 (2-Dodecanoyloxyethyl)trimethylammonium bromide) and DMPM-11 (2-Dodecanoyloxypropyl)trimethylammonium bromide) with a small heme-protein (cytochrome c (cyt-c)) in Hepes buffer (pH = 7.4) were extensively investigated by surface tension, dynamic light scattering (DLS), circular dichroism (CD) and fluorescence spectroscopy in combination with molecular dynamic simulation techniques. The results demonstrated that surfactants can destroy the hydrophobic cavity of cyt-c, make the α-helical become loose and convert it into the β-sheet structure. The interactions between surfactants and cyt-c are mainly hydrophobic. Molecular modelling approaches were also used to gather a deeper insight on the binding of lysosomotropic surfactants with cyt-c and the in silico results were found to be in good agreement with the experimental ones. This study provides a molecular basis for the applications of protein-surfactant complexes in biological, food, pharmaceutical, industrial and cosmetic systems
publishDate 2019
dc.date.none.fl_str_mv 2019-04
2019-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/58393
url http://hdl.handle.net/1822/58393
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Janek, Tomasz; Czelen, Przemyslaw; Gudiña, Eduardo J.; Rodrigues, Lígia R.; Czyznikowska, Zaneta, Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach. International Journal of Biological Macromolecules, 126, 1177-1185, 2019
0141-8130
1879-0003
10.1016/j.ijbiomac.2019.01.024
30625357
https://www.sciencedirect.com/science/article/pii/S0141813018365012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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