Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
| Main Author: | |
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| Publication Date: | 2021 |
| Other Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Download full: | http://hdl.handle.net/10362/113750 |
Summary: | 2018/24643-1 (São Paulo Research Foundation, FAPESP, Brazil), by the National Council for Scientific and Technological Development (Brazil) and by grant UID-BIM-00009-2020 (Foundation for Science and Technology, FCT, Portugal). |
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Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)epigeneticsleadmiRNAncRNAoccupational exposuretoxicity2018/24643-1 (São Paulo Research Foundation, FAPESP, Brazil), by the National Council for Scientific and Technological Development (Brazil) and by grant UID-BIM-00009-2020 (Foundation for Science and Technology, FCT, Portugal).Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centre for Toxicogenomics and Human Health (ToxOmics)RUNAraújo, Marília Ladeira deGomes, Bruno CostaDevóz, Paula PícoliDuarte, Nathália de Assis AguilarRibeiro, Diego LuisAraújo, Adriana Ladeira deBatista, Bruno LemosAntunes, Lusânia Maria GreggiBarbosa, FernandoRodrigues, António SebastiãoRueff, JoséBarcelos, Gustavo Rafael Mazzaron2021-03-11T23:44:05Z2021-02-172021-02-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1application/pdfhttp://hdl.handle.net/10362/113750eng1664-8021PURE: 28133641https://doi.org/10.3389/fgene.2021.620744info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:56:38Zoai:run.unl.pt:10362/113750Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:22.647982Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| title |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| spellingShingle |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) Araújo, Marília Ladeira de epigenetics lead miRNA ncRNA occupational exposure toxicity |
| title_short |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| title_full |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| title_fullStr |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| title_full_unstemmed |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| title_sort |
Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb) |
| author |
Araújo, Marília Ladeira de |
| author_facet |
Araújo, Marília Ladeira de Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis Araújo, Adriana Ladeira de Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron |
| author_role |
author |
| author2 |
Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis Araújo, Adriana Ladeira de Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centre for Toxicogenomics and Human Health (ToxOmics) RUN |
| dc.contributor.author.fl_str_mv |
Araújo, Marília Ladeira de Gomes, Bruno Costa Devóz, Paula Pícoli Duarte, Nathália de Assis Aguilar Ribeiro, Diego Luis Araújo, Adriana Ladeira de Batista, Bruno Lemos Antunes, Lusânia Maria Greggi Barbosa, Fernando Rodrigues, António Sebastião Rueff, José Barcelos, Gustavo Rafael Mazzaron |
| dc.subject.por.fl_str_mv |
epigenetics lead miRNA ncRNA occupational exposure toxicity |
| topic |
epigenetics lead miRNA ncRNA occupational exposure toxicity |
| description |
2018/24643-1 (São Paulo Research Foundation, FAPESP, Brazil), by the National Council for Scientific and Technological Development (Brazil) and by grant UID-BIM-00009-2020 (Foundation for Science and Technology, FCT, Portugal). |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-03-11T23:44:05Z 2021-02-17 2021-02-17T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/113750 |
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http://hdl.handle.net/10362/113750 |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1664-8021 PURE: 28133641 https://doi.org/10.3389/fgene.2021.620744 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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1 application/pdf |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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