Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5864 https://doi.org/10.1016/j.cbi.2006.09.007 |
Resumo: | Atherosclerosis, the main cause of cardiovascular disease (CD), is a chronic inflammatory condition associated with an overproduction of oxidant species, namely peroxynitrite, which is a powerful oxidant that reacts directly with all biomolecules. Glutathione is an efficient scavenger of peroxynitrite, so, modulation of glutathione synthesis may provide a strategy to selectively protect cells from this oxidant. Here, we investigated the ability of resveratrol, a component of red wine, to prevent peroxynitrite-mediated endothelial cells toxicity and the underlying mechanism. Bovine aortic endothelial cells (BAEC) in primary cultures were treated with authentic peroxynitrite and the cell viability and intracellular glutathione contents were assessed. Our results demonstrate that a long pre-incubation (14 h) of BAEC with resveratrol (1-50 [mu]M) leads to the endothelial cells rescue from injury triggered by authentic peroxynitrite by a mechanism of up-regulation of the intracellular GSH content, for the highest resveratrol concentration tested. Considering the importance of GSH in regulation of cell life, this capacity of resveratrol provides a new mechanism for its cardioprotective effects and may contribute to the development of novel therapeutic strategies. |
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Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathioneResveratrolIntracellular glutathionePeroxynitriteCell deathAtherosclerosisAtherosclerosis, the main cause of cardiovascular disease (CD), is a chronic inflammatory condition associated with an overproduction of oxidant species, namely peroxynitrite, which is a powerful oxidant that reacts directly with all biomolecules. Glutathione is an efficient scavenger of peroxynitrite, so, modulation of glutathione synthesis may provide a strategy to selectively protect cells from this oxidant. Here, we investigated the ability of resveratrol, a component of red wine, to prevent peroxynitrite-mediated endothelial cells toxicity and the underlying mechanism. Bovine aortic endothelial cells (BAEC) in primary cultures were treated with authentic peroxynitrite and the cell viability and intracellular glutathione contents were assessed. Our results demonstrate that a long pre-incubation (14 h) of BAEC with resveratrol (1-50 [mu]M) leads to the endothelial cells rescue from injury triggered by authentic peroxynitrite by a mechanism of up-regulation of the intracellular GSH content, for the highest resveratrol concentration tested. Considering the importance of GSH in regulation of cell life, this capacity of resveratrol provides a new mechanism for its cardioprotective effects and may contribute to the development of novel therapeutic strategies.http://www.sciencedirect.com/science/article/B6T56-4M0H8DK-1/1/684245de882520e8b0f0ad01f4aea7662006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5864http://hdl.handle.net/10316/5864https://doi.org/10.1016/j.cbi.2006.09.007engChemico-Biological Interactions. 164:3 (2006) 157-166Brito, Paula M.Mariano, AnabelaAlmeida, Leonor M.Dinis, Teresa C. P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T03:28:58Zoai:estudogeral.uc.pt:10316/5864Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:25.275150Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
title |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
spellingShingle |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione Brito, Paula M. Resveratrol Intracellular glutathione Peroxynitrite Cell death Atherosclerosis |
title_short |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
title_full |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
title_fullStr |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
title_full_unstemmed |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
title_sort |
Resveratrol affords protection against peroxynitrite-mediated endothelial cell death: A role for intracellular glutathione |
author |
Brito, Paula M. |
author_facet |
Brito, Paula M. Mariano, Anabela Almeida, Leonor M. Dinis, Teresa C. P. |
author_role |
author |
author2 |
Mariano, Anabela Almeida, Leonor M. Dinis, Teresa C. P. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Brito, Paula M. Mariano, Anabela Almeida, Leonor M. Dinis, Teresa C. P. |
dc.subject.por.fl_str_mv |
Resveratrol Intracellular glutathione Peroxynitrite Cell death Atherosclerosis |
topic |
Resveratrol Intracellular glutathione Peroxynitrite Cell death Atherosclerosis |
description |
Atherosclerosis, the main cause of cardiovascular disease (CD), is a chronic inflammatory condition associated with an overproduction of oxidant species, namely peroxynitrite, which is a powerful oxidant that reacts directly with all biomolecules. Glutathione is an efficient scavenger of peroxynitrite, so, modulation of glutathione synthesis may provide a strategy to selectively protect cells from this oxidant. Here, we investigated the ability of resveratrol, a component of red wine, to prevent peroxynitrite-mediated endothelial cells toxicity and the underlying mechanism. Bovine aortic endothelial cells (BAEC) in primary cultures were treated with authentic peroxynitrite and the cell viability and intracellular glutathione contents were assessed. Our results demonstrate that a long pre-incubation (14 h) of BAEC with resveratrol (1-50 [mu]M) leads to the endothelial cells rescue from injury triggered by authentic peroxynitrite by a mechanism of up-regulation of the intracellular GSH content, for the highest resveratrol concentration tested. Considering the importance of GSH in regulation of cell life, this capacity of resveratrol provides a new mechanism for its cardioprotective effects and may contribute to the development of novel therapeutic strategies. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5864 http://hdl.handle.net/10316/5864 https://doi.org/10.1016/j.cbi.2006.09.007 |
url |
http://hdl.handle.net/10316/5864 https://doi.org/10.1016/j.cbi.2006.09.007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemico-Biological Interactions. 164:3 (2006) 157-166 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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