Electrochemical detection of in situ adriamycin oxidative damage to DNA

Detalhes bibliográficos
Autor(a) principal: Oliveira-Brett, A. M.
Data de Publicação: 2002
Outros Autores: Vivan, M., Fernandes, I. R., Piedade, J. A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5192
https://doi.org/10.1016/S0039-9140(01)00656-7
Resumo: Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained.
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spelling Electrochemical detection of in situ adriamycin oxidative damage to DNAAdriamycinDNA-biosensorOxidative damage8-OxoguanineVoltammetryAdriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained.http://www.sciencedirect.com/science/article/B6THP-44PVS7R-M/1/1474d369a4b1d556b84d548d2df254392002info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5192http://hdl.handle.net/10316/5192https://doi.org/10.1016/S0039-9140(01)00656-7engTalanta. 56:5 (2002) 959-970Oliveira-Brett, A. M.Vivan, M.Fernandes, I. R.Piedade, J. A. P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:59:33Zoai:estudogeral.uc.pt:10316/5192Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:20.905210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Electrochemical detection of in situ adriamycin oxidative damage to DNA
title Electrochemical detection of in situ adriamycin oxidative damage to DNA
spellingShingle Electrochemical detection of in situ adriamycin oxidative damage to DNA
Oliveira-Brett, A. M.
Adriamycin
DNA-biosensor
Oxidative damage
8-Oxoguanine
Voltammetry
title_short Electrochemical detection of in situ adriamycin oxidative damage to DNA
title_full Electrochemical detection of in situ adriamycin oxidative damage to DNA
title_fullStr Electrochemical detection of in situ adriamycin oxidative damage to DNA
title_full_unstemmed Electrochemical detection of in situ adriamycin oxidative damage to DNA
title_sort Electrochemical detection of in situ adriamycin oxidative damage to DNA
author Oliveira-Brett, A. M.
author_facet Oliveira-Brett, A. M.
Vivan, M.
Fernandes, I. R.
Piedade, J. A. P.
author_role author
author2 Vivan, M.
Fernandes, I. R.
Piedade, J. A. P.
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira-Brett, A. M.
Vivan, M.
Fernandes, I. R.
Piedade, J. A. P.
dc.subject.por.fl_str_mv Adriamycin
DNA-biosensor
Oxidative damage
8-Oxoguanine
Voltammetry
topic Adriamycin
DNA-biosensor
Oxidative damage
8-Oxoguanine
Voltammetry
description Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained.
publishDate 2002
dc.date.none.fl_str_mv 2002
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5192
http://hdl.handle.net/10316/5192
https://doi.org/10.1016/S0039-9140(01)00656-7
url http://hdl.handle.net/10316/5192
https://doi.org/10.1016/S0039-9140(01)00656-7
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Talanta. 56:5 (2002) 959-970
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/PDF
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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