Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/23346 |
Resumo: | Pharmaceutical compounds have been found in rivers and treated wastewaters. They often contaminate irrigation waters and consequently accumulate in edible vegetables, causing changes in plants metabolism The main objective of this work is to understand how lettuce plants cope with the contamination from three selected pharmaceuticals using a label free proteomic analysis. A lettuce hydroponic culture, grown for 36 days, was exposed to metformin, acetaminophen and carbamazepine (at 1 mg/L), during 8 days, after which roots and leaves were sampled and analysed using a liquid chromatography-mass spectrometry proteomics-based approach. In roots, a total of 612 proteins showed differentially accumulation while in leaves 237 proteins were identified with significant differences over controls. Carbamazepine was the contaminant that most affected protein abundance in roots, while in leaves the highest number of differentially accumulated proteins was observed for acetaminophen. In roots under carbamazepine, stress related protein species such as catalase, superoxide dismutase and peroxidases presented higher abundance. Ascorbate peroxidase increased in roots under metformin. Cell respiration protein species were affected by the presence of the three pharmaceuticals suggesting possible dysregulation of the Krebs cycle. Acetaminophen caused the main differences in respiration pathways, with more emphasis in leaves. Lettuce plants revealed different tolerance levels when contaminants were compared, being more tolerant to metformin presence and less tolerant to carbamazepine |
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Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic studylettuceenvironmental contaminationPharmaceutical compounds have been found in rivers and treated wastewaters. They often contaminate irrigation waters and consequently accumulate in edible vegetables, causing changes in plants metabolism The main objective of this work is to understand how lettuce plants cope with the contamination from three selected pharmaceuticals using a label free proteomic analysis. A lettuce hydroponic culture, grown for 36 days, was exposed to metformin, acetaminophen and carbamazepine (at 1 mg/L), during 8 days, after which roots and leaves were sampled and analysed using a liquid chromatography-mass spectrometry proteomics-based approach. In roots, a total of 612 proteins showed differentially accumulation while in leaves 237 proteins were identified with significant differences over controls. Carbamazepine was the contaminant that most affected protein abundance in roots, while in leaves the highest number of differentially accumulated proteins was observed for acetaminophen. In roots under carbamazepine, stress related protein species such as catalase, superoxide dismutase and peroxidases presented higher abundance. Ascorbate peroxidase increased in roots under metformin. Cell respiration protein species were affected by the presence of the three pharmaceuticals suggesting possible dysregulation of the Krebs cycle. Acetaminophen caused the main differences in respiration pathways, with more emphasis in leaves. Lettuce plants revealed different tolerance levels when contaminants were compared, being more tolerant to metformin presence and less tolerant to carbamazepineElsevierRepositório da Universidade de LisboaLeitão, I.Leclerq, C.C.Ribeiro, D.M.Renaut, J.Almeida, A.M.Martins, L.L.Mourato, M.P.2022-02-01T14:25:59Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23346engLeitão I, Leclercq CC, Ribeiro DM, Renaut J, Almeida AM, Martins LL, Mourato MP (2021). Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study. J Proteomics. 2021 Aug 15;245:10429110.1016/j.jprot.2021.104291info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:53Zoai:www.repository.utl.pt:10400.5/23346Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:36.155619Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
title |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
spellingShingle |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study Leitão, I. lettuce environmental contamination |
title_short |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
title_full |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
title_fullStr |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
title_full_unstemmed |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
title_sort |
Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study |
author |
Leitão, I. |
author_facet |
Leitão, I. Leclerq, C.C. Ribeiro, D.M. Renaut, J. Almeida, A.M. Martins, L.L. Mourato, M.P. |
author_role |
author |
author2 |
Leclerq, C.C. Ribeiro, D.M. Renaut, J. Almeida, A.M. Martins, L.L. Mourato, M.P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Leitão, I. Leclerq, C.C. Ribeiro, D.M. Renaut, J. Almeida, A.M. Martins, L.L. Mourato, M.P. |
dc.subject.por.fl_str_mv |
lettuce environmental contamination |
topic |
lettuce environmental contamination |
description |
Pharmaceutical compounds have been found in rivers and treated wastewaters. They often contaminate irrigation waters and consequently accumulate in edible vegetables, causing changes in plants metabolism The main objective of this work is to understand how lettuce plants cope with the contamination from three selected pharmaceuticals using a label free proteomic analysis. A lettuce hydroponic culture, grown for 36 days, was exposed to metformin, acetaminophen and carbamazepine (at 1 mg/L), during 8 days, after which roots and leaves were sampled and analysed using a liquid chromatography-mass spectrometry proteomics-based approach. In roots, a total of 612 proteins showed differentially accumulation while in leaves 237 proteins were identified with significant differences over controls. Carbamazepine was the contaminant that most affected protein abundance in roots, while in leaves the highest number of differentially accumulated proteins was observed for acetaminophen. In roots under carbamazepine, stress related protein species such as catalase, superoxide dismutase and peroxidases presented higher abundance. Ascorbate peroxidase increased in roots under metformin. Cell respiration protein species were affected by the presence of the three pharmaceuticals suggesting possible dysregulation of the Krebs cycle. Acetaminophen caused the main differences in respiration pathways, with more emphasis in leaves. Lettuce plants revealed different tolerance levels when contaminants were compared, being more tolerant to metformin presence and less tolerant to carbamazepine |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-02-01T14:25:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/23346 |
url |
http://hdl.handle.net/10400.5/23346 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Leitão I, Leclercq CC, Ribeiro DM, Renaut J, Almeida AM, Martins LL, Mourato MP (2021). Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study. J Proteomics. 2021 Aug 15;245:104291 10.1016/j.jprot.2021.104291 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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