Simple method for establishing primary Leporidae skin fibroblast cultures

Detalhes bibliográficos
Autor(a) principal: Abade dos Santos, F.A.
Data de Publicação: 2021
Outros Autores: Carvalho, C. L., Almeida, Isabel, FAGULHA, TERESA, Ramos, Fernanda, Barros, Sílvia C., Henriques, Margarida, Luís, Tiago, Duarte, Margarida D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22455
Resumo: Research Areas: Cell Biology
id RCAP_e4156f1974185cae0962f50d5d517ae2
oai_identifier_str oai:www.repository.utl.pt:10400.5/22455
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Simple method for establishing primary Leporidae skin fibroblast culturesPrimary fibroblastsPrimary cell cultureVirus isolationMethodDispase IILeporidaeResearch Areas: Cell BiologyCommercial hare and rabbit immortalized cell lines are extremely limited regarding the many species within the lagomorpha order. To overcome this limitation, researchers and technicians must establish primary cell cultures derived from biopsies or embryos. Among all cell types, fibroblasts are plastic and resilient cells, highly convenient for clinical and fundamental research but also for diagnosis, particularly for viral isolation. Here, we describe a fast and cheap method to produce primary fibroblast cell cultures from leporid species, using dispase II, a protease that allows dermal–epidermal separation, followed by a simple enzymatic digestion with trypsin. This method allows for the establishment of an in vitro cell culture system with an excellent viability yield and purity level higher than 85% and enables the maintenance and even immortalization of leporid fibroblastic cells derived from tissues already differentiated.MDPIRepositório da Universidade de LisboaAbade dos Santos, F.A.Carvalho, C. L.Almeida, IsabelFAGULHA, TERESARamos, FernandaBarros, Sílvia C.Henriques, MargaridaLuís, TiagoDuarte, Margarida D.2021-11-01T00:41:28Z2021-08-162021-08-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22455engAbade dos Santos FA, Carvalho CL, Almeida I, Fagulha T, Ramos F, Barros SC, Henriques M, Luís T, Duarte MD. 2021. Simple method for establishing primary Leporidae skin fibroblast cultures. Cells 10(8). doi.org/10.3390/cells1008210010.3390/cells100821002073-4409info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:59Zoai:www.repository.utl.pt:10400.5/22455Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:52.659749Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Simple method for establishing primary Leporidae skin fibroblast cultures
title Simple method for establishing primary Leporidae skin fibroblast cultures
spellingShingle Simple method for establishing primary Leporidae skin fibroblast cultures
Abade dos Santos, F.A.
Primary fibroblasts
Primary cell culture
Virus isolation
Method
Dispase II
Leporidae
title_short Simple method for establishing primary Leporidae skin fibroblast cultures
title_full Simple method for establishing primary Leporidae skin fibroblast cultures
title_fullStr Simple method for establishing primary Leporidae skin fibroblast cultures
title_full_unstemmed Simple method for establishing primary Leporidae skin fibroblast cultures
title_sort Simple method for establishing primary Leporidae skin fibroblast cultures
author Abade dos Santos, F.A.
author_facet Abade dos Santos, F.A.
Carvalho, C. L.
Almeida, Isabel
FAGULHA, TERESA
Ramos, Fernanda
Barros, Sílvia C.
Henriques, Margarida
Luís, Tiago
Duarte, Margarida D.
author_role author
author2 Carvalho, C. L.
Almeida, Isabel
FAGULHA, TERESA
Ramos, Fernanda
Barros, Sílvia C.
Henriques, Margarida
Luís, Tiago
Duarte, Margarida D.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Abade dos Santos, F.A.
Carvalho, C. L.
Almeida, Isabel
FAGULHA, TERESA
Ramos, Fernanda
Barros, Sílvia C.
Henriques, Margarida
Luís, Tiago
Duarte, Margarida D.
dc.subject.por.fl_str_mv Primary fibroblasts
Primary cell culture
Virus isolation
Method
Dispase II
Leporidae
topic Primary fibroblasts
Primary cell culture
Virus isolation
Method
Dispase II
Leporidae
description Research Areas: Cell Biology
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01T00:41:28Z
2021-08-16
2021-08-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22455
url http://hdl.handle.net/10400.5/22455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Abade dos Santos FA, Carvalho CL, Almeida I, Fagulha T, Ramos F, Barros SC, Henriques M, Luís T, Duarte MD. 2021. Simple method for establishing primary Leporidae skin fibroblast cultures. Cells 10(8). doi.org/10.3390/cells10082100
10.3390/cells10082100
2073-4409
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131162005733376

Registros relacionados