Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status

Detalhes bibliográficos
Autor(a) principal: Coelho, Pedro
Data de Publicação: 2017
Outros Autores: Silva, Liliana, Faria, Isabel, Vieira, Mónica, Monteiro, Armanda, Pinto, Gabriela, Prudêncio, Cristina, Fernandes, Rúben, Soares, Raquel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/18926
Resumo: Radiotherapy is a treatment option for the majority of malignancies. However, because melanoma is known to be radioresistant, the use of ionizing radiation as an adjuvant therapy in cutaneous melanoma patients is ineffective. Obesity has now been recognized as a risk factor for melanoma. High adiposity is generally associated with a more pro-oxidative status. Oxidative stress is a major player in radiation therapy and also a common link between obesity and cancer. Several adipocyte-released proteins are known to have a role in controlling cellular growth and pro-survival signaling. For that reason, we investigated the influence of 3T3-L1 mature adipocyte secretome in B16-F10 malignant melanocyte radiosensitivity. We evaluated B16-F10 cell survival and redox homeostasis when exposed to four daily doses of ionizing radiation (2 Gy per day) up to a total of 8 Gy in a medical linear accelerator. B16-F10 melanocytes exhibited slight alterations in survival, catalase activity, nitrative stress and total oxidant concentration after the first 2 Gy irradiation. The motility of the melanocytes was also delayed by ionizing radiation. Subsequent irradiations of the malignant melanocytes led to more prominent reductions in overall survival. Remarkably, 3T3-L1 adipocyte-secreted molecules were able to increase the viability and migration of melanocytes, as well as lessen the pro-oxidant burden induced by both the single and cumulative X-ray doses. In vitro adipocyte-released factors protected B16-F10 malignant melanocytes from both oxidative stress and loss of viability triggered by radiation, enhancing the radioresistant phenoyype of these cells with a concomitant activation of the AKT signaling pathway These results both help to elucidate how obesity influences melanoma radioresistance and support the usage of conventional medical linear accelerators as a valid model for the in vitro radiobiological study of tumor cell lines.
id RCAP_e42dd6a8348d75a1a60b7d30cbbc88d8
oai_identifier_str oai:recipp.ipp.pt:10400.22/18926
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative statusAdipocyte secretomeMalignant melanocytesRadiotherapy is a treatment option for the majority of malignancies. However, because melanoma is known to be radioresistant, the use of ionizing radiation as an adjuvant therapy in cutaneous melanoma patients is ineffective. Obesity has now been recognized as a risk factor for melanoma. High adiposity is generally associated with a more pro-oxidative status. Oxidative stress is a major player in radiation therapy and also a common link between obesity and cancer. Several adipocyte-released proteins are known to have a role in controlling cellular growth and pro-survival signaling. For that reason, we investigated the influence of 3T3-L1 mature adipocyte secretome in B16-F10 malignant melanocyte radiosensitivity. We evaluated B16-F10 cell survival and redox homeostasis when exposed to four daily doses of ionizing radiation (2 Gy per day) up to a total of 8 Gy in a medical linear accelerator. B16-F10 melanocytes exhibited slight alterations in survival, catalase activity, nitrative stress and total oxidant concentration after the first 2 Gy irradiation. The motility of the melanocytes was also delayed by ionizing radiation. Subsequent irradiations of the malignant melanocytes led to more prominent reductions in overall survival. Remarkably, 3T3-L1 adipocyte-secreted molecules were able to increase the viability and migration of melanocytes, as well as lessen the pro-oxidant burden induced by both the single and cumulative X-ray doses. In vitro adipocyte-released factors protected B16-F10 malignant melanocytes from both oxidative stress and loss of viability triggered by radiation, enhancing the radioresistant phenoyype of these cells with a concomitant activation of the AKT signaling pathway These results both help to elucidate how obesity influences melanoma radioresistance and support the usage of conventional medical linear accelerators as a valid model for the in vitro radiobiological study of tumor cell lines.Radiation Research SocietyRepositório Científico do Instituto Politécnico do PortoCoelho, PedroSilva, LilianaFaria, IsabelVieira, MónicaMonteiro, ArmandaPinto, GabrielaPrudêncio, CristinaFernandes, RúbenSoares, Raquel2021-11-22T13:56:04Z2017-05-012017-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18926engCoelho, P., Silva, L., Faria, I., Vieria, M., Monteiro, A., Pinto, G., Prudêncio, C., Fernandes, R., & Soares, R. (2017). Adipocyte Secretome Increases Radioresistance of Malignant Melanocytes by Improving Cell Survival and Decreasing Oxidative Status. Radiation Research, 187(5), 581-588. https://doi.org/10.1667/rr14551.110.1667/RR14551.11938-5404info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:12:10Zoai:recipp.ipp.pt:10400.22/18926Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:58.335847Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
title Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
spellingShingle Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
Coelho, Pedro
Adipocyte secretome
Malignant melanocytes
title_short Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
title_full Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
title_fullStr Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
title_full_unstemmed Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
title_sort Adipocyte secretome increases radioresistance of malignant melanocytes by improving cell survival and decreasing oxidative status
author Coelho, Pedro
author_facet Coelho, Pedro
Silva, Liliana
Faria, Isabel
Vieira, Mónica
Monteiro, Armanda
Pinto, Gabriela
Prudêncio, Cristina
Fernandes, Rúben
Soares, Raquel
author_role author
author2 Silva, Liliana
Faria, Isabel
Vieira, Mónica
Monteiro, Armanda
Pinto, Gabriela
Prudêncio, Cristina
Fernandes, Rúben
Soares, Raquel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Coelho, Pedro
Silva, Liliana
Faria, Isabel
Vieira, Mónica
Monteiro, Armanda
Pinto, Gabriela
Prudêncio, Cristina
Fernandes, Rúben
Soares, Raquel
dc.subject.por.fl_str_mv Adipocyte secretome
Malignant melanocytes
topic Adipocyte secretome
Malignant melanocytes
description Radiotherapy is a treatment option for the majority of malignancies. However, because melanoma is known to be radioresistant, the use of ionizing radiation as an adjuvant therapy in cutaneous melanoma patients is ineffective. Obesity has now been recognized as a risk factor for melanoma. High adiposity is generally associated with a more pro-oxidative status. Oxidative stress is a major player in radiation therapy and also a common link between obesity and cancer. Several adipocyte-released proteins are known to have a role in controlling cellular growth and pro-survival signaling. For that reason, we investigated the influence of 3T3-L1 mature adipocyte secretome in B16-F10 malignant melanocyte radiosensitivity. We evaluated B16-F10 cell survival and redox homeostasis when exposed to four daily doses of ionizing radiation (2 Gy per day) up to a total of 8 Gy in a medical linear accelerator. B16-F10 melanocytes exhibited slight alterations in survival, catalase activity, nitrative stress and total oxidant concentration after the first 2 Gy irradiation. The motility of the melanocytes was also delayed by ionizing radiation. Subsequent irradiations of the malignant melanocytes led to more prominent reductions in overall survival. Remarkably, 3T3-L1 adipocyte-secreted molecules were able to increase the viability and migration of melanocytes, as well as lessen the pro-oxidant burden induced by both the single and cumulative X-ray doses. In vitro adipocyte-released factors protected B16-F10 malignant melanocytes from both oxidative stress and loss of viability triggered by radiation, enhancing the radioresistant phenoyype of these cells with a concomitant activation of the AKT signaling pathway These results both help to elucidate how obesity influences melanoma radioresistance and support the usage of conventional medical linear accelerators as a valid model for the in vitro radiobiological study of tumor cell lines.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
2017-05-01T00:00:00Z
2021-11-22T13:56:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/18926
url http://hdl.handle.net/10400.22/18926
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Coelho, P., Silva, L., Faria, I., Vieria, M., Monteiro, A., Pinto, G., Prudêncio, C., Fernandes, R., & Soares, R. (2017). Adipocyte Secretome Increases Radioresistance of Malignant Melanocytes by Improving Cell Survival and Decreasing Oxidative Status. Radiation Research, 187(5), 581-588. https://doi.org/10.1667/rr14551.1
10.1667/RR14551.1
1938-5404
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Radiation Research Society
publisher.none.fl_str_mv Radiation Research Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131477940633600