Purines and nucleic acids removal with materials functionalized with ionic liquids

• Autor(a) principal: Simões, Johanna Madeira
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/29461
• topic: Materials functionalized with ionic liquids; Liquids; Adsorption; DNA removal; Gout

Purines and nucleic acid are present in several fermented beverages, including beer. These compounds essentially come from malt and yeast DNA. When ingested by humans they are catabolized into uric acid, contributing to its serum level increase leading to hyperuricemia, a risk factor of gout. This disease causes inflammation and intense pain and can be acute or chronic. For this reason, in the treatment of gout, it is recommended a diet with beer restrictions. Therefore, it is relevant to develop methodologies that can remove purines and their precursors from beer, in this way the Supported Ionic Liquids (SIL) that are studied for the adsorption of purine compounds and DNA Saccharomyces cerevisiae from aqueous solutions. Five SILs with different cations and chloride as counter ion were synthesized and characterized using several techniques: Elemental analysis, Fourier-Transform Infrared spectroscopy with attenuated Total Reflectance, Solid-State 13C NMR, point of zero charges, Surface area and pore structure characterization and Scanning electron microscope. Then the materials are used for purines, DNA and amino acids assays- Tyrosine. SILs demonstrate no capacity to remove purines. On the other hand, the DNA extracted from Saccharomyces cerevisiae was used to performed adsorption assay. The data demonstrate that DNA adsorption is instantaneous, in this way, the models of kinetics are not applicable. Of the five synthesized SILs, [Si][C3C1Im]Cl; [Si][N3114]Cl and [Si][N3222]Cl showed the ability to remove DNA. [Si][C3C1Im]Cl demonstrates to be the most efficient for DNA removal with a capacity of removal 43.32 μg per mg of material. These SILs don’t demonstrate the adsorbed capacity for this amino acid removal, giving a little proof of selectivity. Further studies are being carried out in order to identify more promising materials that can be used to remove purine compounds and DNA from beer to avoid beer restrictions due to hyperuricemia and gout conditions.