Antifungal and anti-biofilm activity of designed derivatives from kyotorphin

Detalhes bibliográficos
Autor(a) principal: Martins de Andrade, Vitor
Data de Publicação: 2020
Outros Autores: Bardají, Eduard, Heras, Montserrat, Ramu, Vasanthakumar G., Junqueira, Juliana Campos, Diane dos Santos, Jéssica, Castanho, Miguel A. R. B., Conceição, Katia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/57337
Resumo: © 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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spelling Antifungal and anti-biofilm activity of designed derivatives from kyotorphinAnalgesic peptideAntibiofilmAntimicrobial peptidesToxicity© 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.Kyotorphin (KTP, l-tyrosyl-l-arginine) is an endogenous analgesic neuropeptide first isolated from bovine brain in 1979. Previous studies have shown that kyotorphins possess anti-inflammatory and antimicrobial activity. Six kyotorphins—KTP-NH2, KTP–NH2–DL, ibuprofen-conjugated KTP (IbKTP), IbKTP-NH2, N-methyl-D-Tyr-L-Arg, and N-methyl-L-Tyr-D-Arg—were designed and synthesized to improve lipophilicity and resistance to enzymatic degradation. This study assessed the antimicrobial and antibiofilm activity of these peptides. The antifungal activity of kyotorphins was determined in representative strains of Candida species, including Candida albicans ATCC 10231, Candida krusei ATCC 6258, and six clinical isolates—Candida dubliniensis 19-S, Candida glabrata 217-S, Candida lusitaniae 14-S, Candida novergensis 51-S, Candida parapsilosis 63, and Candida tropicalis 140-S—obtained from the oral cavity of HIV-positive patients. The peptides were synthesized by standard solution or solid-phase synthesis, purified by RP-HPLC (purity >95 %), and characterized by nuclear magnetic resonance. The results of the broth microdilution assay and scanning electron microscopy showed that IbKTP-NH2 presented significant antifungal activity against Candida strains and antibiofilm activity against the clinical isolates. The absence of toxic activity and survival after infection was assessed after injecting the peptide in larvae of Galleria mellonella as experimental infection model. Furthermore, IbKTP-NH2 had strong antimicrobial activity against multidrug-resistant bacteria and fungi and was not toxic to G. mellonella larvae up to a concentration of 500 mM. These results suggest that IbKTP-NH2, in addition to its known effect on cell membranes, can elicit a cellular immune response and, therefore, is promising for biomedical application.This research was supported by FAPESP (Grant No. 2017/00032-0). This article is also part of the Fungal Adaptation to Hostile Challenges special issue for the third International Symposium on Fungal Stress (ISFUS), which is supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant No. 2018/20571-6) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Grant No. 88881.289327/2018-01).ElsevierRepositório da Universidade de LisboaMartins de Andrade, VitorBardají, EduardHeras, MontserratRamu, Vasanthakumar G.Junqueira, Juliana CamposDiane dos Santos, JéssicaCastanho, Miguel A. R. B.Conceição, Katia2023-05-03T14:58:20Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57337eng1878-614610.1016/j.funbio.2019.12.0021878-6162info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:05:30Zoai:repositorio.ul.pt:10451/57337Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:45.630025Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
title Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
spellingShingle Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
Martins de Andrade, Vitor
Analgesic peptide
Antibiofilm
Antimicrobial peptides
Toxicity
title_short Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
title_full Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
title_fullStr Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
title_full_unstemmed Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
title_sort Antifungal and anti-biofilm activity of designed derivatives from kyotorphin
author Martins de Andrade, Vitor
author_facet Martins de Andrade, Vitor
Bardají, Eduard
Heras, Montserrat
Ramu, Vasanthakumar G.
Junqueira, Juliana Campos
Diane dos Santos, Jéssica
Castanho, Miguel A. R. B.
Conceição, Katia
author_role author
author2 Bardají, Eduard
Heras, Montserrat
Ramu, Vasanthakumar G.
Junqueira, Juliana Campos
Diane dos Santos, Jéssica
Castanho, Miguel A. R. B.
Conceição, Katia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Martins de Andrade, Vitor
Bardají, Eduard
Heras, Montserrat
Ramu, Vasanthakumar G.
Junqueira, Juliana Campos
Diane dos Santos, Jéssica
Castanho, Miguel A. R. B.
Conceição, Katia
dc.subject.por.fl_str_mv Analgesic peptide
Antibiofilm
Antimicrobial peptides
Toxicity
topic Analgesic peptide
Antibiofilm
Antimicrobial peptides
Toxicity
description © 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2023-05-03T14:58:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/57337
url http://hdl.handle.net/10451/57337
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1878-6146
10.1016/j.funbio.2019.12.002
1878-6162
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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