Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/143275 |
Resumo: | EUTOPIA CA17139 Project N.ffi 21874 COMPETE 2020 nffi 246/AXIS II/2017 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 CellsSDG 9 - Industry, Innovation, and InfrastructureEUTOPIA CA17139 Project N.ffi 21874 COMPETE 2020 nffi 246/AXIS II/2017Nanocellulose is an innovative engineered nanomaterial with an enormous potential for use in a wide array of industrial and biomedical applications and with fast growing economic value. The expanding production of nanocellulose is leading to an increased human exposure, raising concerns about their potential health effects. This study was aimed at assessing the potential toxic and genotoxic effects of different nanocelluloses in two mammalian cell lines; (2) Methods: Two micro/nanocelluloses, produced with a TEMPO oxidation pre-treatment (CNFs) and an enzymatic pre-treatment (CMFs), and cellulose nanocrystals (CNCs) were tested in osteoblastic-like human cells (MG-63) and Chinese hamster lung fibroblasts (V79) using the MTT and clonogenic assays to analyse cytotoxicity, and the micronucleus assay to test genotoxicity; (3) Results: cytotoxicity was observed by the clonogenic assay in V79 cells, particularly for CNCs, but not by the MTT assay; CNF induced micronuclei in both cell lines and nucleoplasmic bridges in MG-63 cells; CMF and CNC induced micronuclei and nucleoplasmic bridges in MG-63 cells, but not in V79 cells; (4) Conclusions: All nanocelluloses revealed cytotoxicity and genotoxicity, although at different concentrations, that may be related to their physicochemical differences and availability for cell uptake, and to differences in the DNA damage response of the cell model.Centre for Toxicogenomics and Human Health (ToxOmics)DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)RUNVentura, CéliaMarques, CatarinaCadete, JoãoVilar, MadalenaPedrosa, Jorge F. S.Pinto, FátimaFernandes, Susete NogueiraRosa, Rafaela Raupp daGodinho, Maria HelenaFerreira, Paulo J. T.Louro, HenriquetaSilva, Maria João2022-08-24T22:17:00Z2022-04-212022-04-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/143275eng2039-4713PURE: 44853982https://doi.org/10.3390/jox12020009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:21:34Zoai:run.unl.pt:10362/143275Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:48.236069Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| spellingShingle |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells Ventura, Célia SDG 9 - Industry, Innovation, and Infrastructure |
| title_short |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_full |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_fullStr |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_full_unstemmed |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_sort |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| author |
Ventura, Célia |
| author_facet |
Ventura, Célia Marques, Catarina Cadete, João Vilar, Madalena Pedrosa, Jorge F. S. Pinto, Fátima Fernandes, Susete Nogueira Rosa, Rafaela Raupp da Godinho, Maria Helena Ferreira, Paulo J. T. Louro, Henriqueta Silva, Maria João |
| author_role |
author |
| author2 |
Marques, Catarina Cadete, João Vilar, Madalena Pedrosa, Jorge F. S. Pinto, Fátima Fernandes, Susete Nogueira Rosa, Rafaela Raupp da Godinho, Maria Helena Ferreira, Paulo J. T. Louro, Henriqueta Silva, Maria João |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Centre for Toxicogenomics and Human Health (ToxOmics) DCM - Departamento de Ciência dos Materiais CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) RUN |
| dc.contributor.author.fl_str_mv |
Ventura, Célia Marques, Catarina Cadete, João Vilar, Madalena Pedrosa, Jorge F. S. Pinto, Fátima Fernandes, Susete Nogueira Rosa, Rafaela Raupp da Godinho, Maria Helena Ferreira, Paulo J. T. Louro, Henriqueta Silva, Maria João |
| dc.subject.por.fl_str_mv |
SDG 9 - Industry, Innovation, and Infrastructure |
| topic |
SDG 9 - Industry, Innovation, and Infrastructure |
| description |
EUTOPIA CA17139 Project N.ffi 21874 COMPETE 2020 nffi 246/AXIS II/2017 |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-08-24T22:17:00Z 2022-04-21 2022-04-21T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/143275 |
| url |
http://hdl.handle.net/10362/143275 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2039-4713 PURE: 44853982 https://doi.org/10.3390/jox12020009 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
18 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138103888183296 |