Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/43908 |
Resumo: | Although the diversity of bacterial endosymbionts in arthropods is well documented, whether and how such diversity is maintained remains an open question. We investigated the temporal changes occurring in the prevalence and composition of endosymbionts after transferring natural populations of Tetranychus spider mites from the field to the laboratory. These populations, belonging to three different Tetranychus species (T. urticae, T. ludeni and T. evansi) carried variable infection frequencies of Wolbachia, Cardinium, and Rickettsia. We report a rapid change of the infection status of these populations after only 6 months of laboratory rearing, with an apparent loss of Rickettsia and Cardinium, while Wolbachia apparently either reached fixation or was lost. We show that Wolbachia had variable effects on host longevity and fecundity, and induced variable levels of cytoplasmic incompatibility (CI) in each fully infected population, despite no sequence divergence in the markers used and full CI rescue between all populations. This suggests that such effects are largely dependent upon the host genotype. Subsequently, we used these data to parameterize a theoretical model for the invasion of CI-inducing symbionts in haplodiploids, which shows that symbiont effects are sufficient to explain their dynamics in the laboratory. This further suggests that symbiont diversity and prevalence in the field are likely maintained by environmental heterogeneity, which is reduced in the laboratory. Overall, this study highlights the lability of endosymbiont infections and draws attention to the limitations of laboratory studies to understand host-symbiont interactions in natural populations. |
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Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditionsEvolutionary EcologyMicrobial EcologyEndosymbiontTetranychusHost–symbiont interactionLaboratory adaptationAlthough the diversity of bacterial endosymbionts in arthropods is well documented, whether and how such diversity is maintained remains an open question. We investigated the temporal changes occurring in the prevalence and composition of endosymbionts after transferring natural populations of Tetranychus spider mites from the field to the laboratory. These populations, belonging to three different Tetranychus species (T. urticae, T. ludeni and T. evansi) carried variable infection frequencies of Wolbachia, Cardinium, and Rickettsia. We report a rapid change of the infection status of these populations after only 6 months of laboratory rearing, with an apparent loss of Rickettsia and Cardinium, while Wolbachia apparently either reached fixation or was lost. We show that Wolbachia had variable effects on host longevity and fecundity, and induced variable levels of cytoplasmic incompatibility (CI) in each fully infected population, despite no sequence divergence in the markers used and full CI rescue between all populations. This suggests that such effects are largely dependent upon the host genotype. Subsequently, we used these data to parameterize a theoretical model for the invasion of CI-inducing symbionts in haplodiploids, which shows that symbiont effects are sufficient to explain their dynamics in the laboratory. This further suggests that symbiont diversity and prevalence in the field are likely maintained by environmental heterogeneity, which is reduced in the laboratory. Overall, this study highlights the lability of endosymbiont infections and draws attention to the limitations of laboratory studies to understand host-symbiont interactions in natural populations.Springer NatureRepositório da Universidade de LisboaZélé, FloreSantos, InêsMatos, MargaridaWeill, MylèneVavre, FabriceMagalhães, sara2020-08-11T00:30:14Z2020-02-112020-02-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/43908engZELÉ, F., SANTOS, I., MATOS, M., VAVRE, F., WEILL, M. & MAGALHÃES, S. 2020. Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions. Heredity 124: 603–617. DOI: https://doi.org/10.1038/s41437-020-0297-910.1038/s41437-020-0297-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:44:35Zoai:repositorio.ul.pt:10451/43908Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:56:37.315662Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
title |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
spellingShingle |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions Zélé, Flore Evolutionary Ecology Microbial Ecology Endosymbiont Tetranychus Host–symbiont interaction Laboratory adaptation |
title_short |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
title_full |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
title_fullStr |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
title_full_unstemmed |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
title_sort |
Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions |
author |
Zélé, Flore |
author_facet |
Zélé, Flore Santos, Inês Matos, Margarida Weill, Mylène Vavre, Fabrice Magalhães, sara |
author_role |
author |
author2 |
Santos, Inês Matos, Margarida Weill, Mylène Vavre, Fabrice Magalhães, sara |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Zélé, Flore Santos, Inês Matos, Margarida Weill, Mylène Vavre, Fabrice Magalhães, sara |
dc.subject.por.fl_str_mv |
Evolutionary Ecology Microbial Ecology Endosymbiont Tetranychus Host–symbiont interaction Laboratory adaptation |
topic |
Evolutionary Ecology Microbial Ecology Endosymbiont Tetranychus Host–symbiont interaction Laboratory adaptation |
description |
Although the diversity of bacterial endosymbionts in arthropods is well documented, whether and how such diversity is maintained remains an open question. We investigated the temporal changes occurring in the prevalence and composition of endosymbionts after transferring natural populations of Tetranychus spider mites from the field to the laboratory. These populations, belonging to three different Tetranychus species (T. urticae, T. ludeni and T. evansi) carried variable infection frequencies of Wolbachia, Cardinium, and Rickettsia. We report a rapid change of the infection status of these populations after only 6 months of laboratory rearing, with an apparent loss of Rickettsia and Cardinium, while Wolbachia apparently either reached fixation or was lost. We show that Wolbachia had variable effects on host longevity and fecundity, and induced variable levels of cytoplasmic incompatibility (CI) in each fully infected population, despite no sequence divergence in the markers used and full CI rescue between all populations. This suggests that such effects are largely dependent upon the host genotype. Subsequently, we used these data to parameterize a theoretical model for the invasion of CI-inducing symbionts in haplodiploids, which shows that symbiont effects are sufficient to explain their dynamics in the laboratory. This further suggests that symbiont diversity and prevalence in the field are likely maintained by environmental heterogeneity, which is reduced in the laboratory. Overall, this study highlights the lability of endosymbiont infections and draws attention to the limitations of laboratory studies to understand host-symbiont interactions in natural populations. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-11T00:30:14Z 2020-02-11 2020-02-11T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/43908 |
url |
http://hdl.handle.net/10451/43908 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ZELÉ, F., SANTOS, I., MATOS, M., VAVRE, F., WEILL, M. & MAGALHÃES, S. 2020. Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions. Heredity 124: 603–617. DOI: https://doi.org/10.1038/s41437-020-0297-9 10.1038/s41437-020-0297-9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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