Multifunctional laminarin microparticles for cell adhesion and expansion

Detalhes bibliográficos
Autor(a) principal: Martins, C. R.
Data de Publicação: 2018
Outros Autores: Custódio, C. A., Mano, J. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/25664
Resumo: Microfabrication technologies have been widely explored to produce microgels that can be assembled in functional constructs for tissue engineering and regenerative medicine applications. Here, we propose microfluidics coupled to a source of UV light to produce multifunctional methacrylated laminarin microparticles with narrow distribution of sizes using photopolymerization. The multifunctional microparticles were loaded with platelet lysates and further conjugated with an adhesive peptide. The adhesive peptides dictated cell adhesiveness to the laminarin microparticles, the incorporation of platelet lysates have resulted in improved cell expansion compared to clear microparticles. Overall, our findings demonstrate that multifunctional methacrylated laminarin microparticles provide an effective support for cell attachment and expansion. Moreover, expanded cells provide the link for microparticles aggregation resulting in robust 3D structures. This suggest the potential for using the methacrylated laminarin microplatforms capable to be assembled by the action of cells to rapidly produce large tissue engineered constructs.
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spelling Multifunctional laminarin microparticles for cell adhesion and expansionInjectable scaffoldsMethacrylated laminarinMicrocarrierMicrofluidicMicrogelsPlatelet lysatesMicrofabrication technologies have been widely explored to produce microgels that can be assembled in functional constructs for tissue engineering and regenerative medicine applications. Here, we propose microfluidics coupled to a source of UV light to produce multifunctional methacrylated laminarin microparticles with narrow distribution of sizes using photopolymerization. The multifunctional microparticles were loaded with platelet lysates and further conjugated with an adhesive peptide. The adhesive peptides dictated cell adhesiveness to the laminarin microparticles, the incorporation of platelet lysates have resulted in improved cell expansion compared to clear microparticles. Overall, our findings demonstrate that multifunctional methacrylated laminarin microparticles provide an effective support for cell attachment and expansion. Moreover, expanded cells provide the link for microparticles aggregation resulting in robust 3D structures. This suggest the potential for using the methacrylated laminarin microplatforms capable to be assembled by the action of cells to rapidly produce large tissue engineered constructs.Elsevier2019-12-31T00:00:00Z2018-12-15T00:00:00Z2018-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://hdl.handle.net/10773/25664eng0144-861710.1016/j.carbpol.2018.08.029Martins, C. R.Custódio, C. A.Mano, J. F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:49:43Zoai:ria.ua.pt:10773/25664Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:58:50.178717Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Multifunctional laminarin microparticles for cell adhesion and expansion
title Multifunctional laminarin microparticles for cell adhesion and expansion
spellingShingle Multifunctional laminarin microparticles for cell adhesion and expansion
Martins, C. R.
Injectable scaffolds
Methacrylated laminarin
Microcarrier
Microfluidic
Microgels
Platelet lysates
title_short Multifunctional laminarin microparticles for cell adhesion and expansion
title_full Multifunctional laminarin microparticles for cell adhesion and expansion
title_fullStr Multifunctional laminarin microparticles for cell adhesion and expansion
title_full_unstemmed Multifunctional laminarin microparticles for cell adhesion and expansion
title_sort Multifunctional laminarin microparticles for cell adhesion and expansion
author Martins, C. R.
author_facet Martins, C. R.
Custódio, C. A.
Mano, J. F.
author_role author
author2 Custódio, C. A.
Mano, J. F.
author2_role author
author
dc.contributor.author.fl_str_mv Martins, C. R.
Custódio, C. A.
Mano, J. F.
dc.subject.por.fl_str_mv Injectable scaffolds
Methacrylated laminarin
Microcarrier
Microfluidic
Microgels
Platelet lysates
topic Injectable scaffolds
Methacrylated laminarin
Microcarrier
Microfluidic
Microgels
Platelet lysates
description Microfabrication technologies have been widely explored to produce microgels that can be assembled in functional constructs for tissue engineering and regenerative medicine applications. Here, we propose microfluidics coupled to a source of UV light to produce multifunctional methacrylated laminarin microparticles with narrow distribution of sizes using photopolymerization. The multifunctional microparticles were loaded with platelet lysates and further conjugated with an adhesive peptide. The adhesive peptides dictated cell adhesiveness to the laminarin microparticles, the incorporation of platelet lysates have resulted in improved cell expansion compared to clear microparticles. Overall, our findings demonstrate that multifunctional methacrylated laminarin microparticles provide an effective support for cell attachment and expansion. Moreover, expanded cells provide the link for microparticles aggregation resulting in robust 3D structures. This suggest the potential for using the methacrylated laminarin microplatforms capable to be assembled by the action of cells to rapidly produce large tissue engineered constructs.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-15T00:00:00Z
2018-12-15
2019-12-31T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/25664
url http://hdl.handle.net/10773/25664
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0144-8617
10.1016/j.carbpol.2018.08.029
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/vnd.openxmlformats-officedocument.wordprocessingml.document
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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