New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/13590 |
Resumo: | The physiological/structural status of the soft ripened berry is still a matter of debate. In this paper isolated mesocarp cells from ripened berries of both wine and table varieties were studied by bright-field, fluorescence and confocal microscopy and flow cytometry to highlight the organization of berry flesh cell, function and viability. Flow cytometry analysis confirmed that protoplasting from grape berry mesocarp tissue yields a single heterogenous population of intact and viable cells. Also, the integrity of the plasma membrane and the architecture and complexity of the intracellular membranous system were shown by FM1-43 staining coupled to confocal microscopy imaging. The observed incorporation of the fluorescent glucose analogue 2-NBDG suggests that endocytosis is involved in the transport and intracellular compartmentation of apoplastic sugars. Neutral Red staining confirmed the intricate organization, size, diversity and integrity of the vacuolar apparatus that is probably related to the multifaceted roles of the vacuoles in the developing fruit. |
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New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berryMesocarp cellsProtoplastsCell viabilityCell compartmentationGrape berry ripeningScience & TechnologyThe physiological/structural status of the soft ripened berry is still a matter of debate. In this paper isolated mesocarp cells from ripened berries of both wine and table varieties were studied by bright-field, fluorescence and confocal microscopy and flow cytometry to highlight the organization of berry flesh cell, function and viability. Flow cytometry analysis confirmed that protoplasting from grape berry mesocarp tissue yields a single heterogenous population of intact and viable cells. Also, the integrity of the plasma membrane and the architecture and complexity of the intracellular membranous system were shown by FM1-43 staining coupled to confocal microscopy imaging. The observed incorporation of the fluorescent glucose analogue 2-NBDG suggests that endocytosis is involved in the transport and intracellular compartmentation of apoplastic sugars. Neutral Red staining confirmed the intricate organization, size, diversity and integrity of the vacuolar apparatus that is probably related to the multifaceted roles of the vacuoles in the developing fruit.This work was supported in part by the Fundação para a Ciência e a Tecnologia (research projects PTDC/AGR-ALI/100636/2008 and PTDC/BIA-BCM/69448/2006 and grant no. SFRH/BD/23169/2005 to N.F).American Society for Enology and Viticulture (ASEV)Universidade do MinhoFontes, N.Côrte-Real, ManuelaGerós, H.2011-092011-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/13590eng0002-925410.5344/ajev.2011.10126The original publication is available at http://ajevonline.org/cgi/content/abstract/62/3/279info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:53Zoai:repositorium.sdum.uminho.pt:1822/13590Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:53.620919Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
title |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
spellingShingle |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry Fontes, N. Mesocarp cells Protoplasts Cell viability Cell compartmentation Grape berry ripening Science & Technology |
title_short |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
title_full |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
title_fullStr |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
title_full_unstemmed |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
title_sort |
New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry |
author |
Fontes, N. |
author_facet |
Fontes, N. Côrte-Real, Manuela Gerós, H. |
author_role |
author |
author2 |
Côrte-Real, Manuela Gerós, H. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fontes, N. Côrte-Real, Manuela Gerós, H. |
dc.subject.por.fl_str_mv |
Mesocarp cells Protoplasts Cell viability Cell compartmentation Grape berry ripening Science & Technology |
topic |
Mesocarp cells Protoplasts Cell viability Cell compartmentation Grape berry ripening Science & Technology |
description |
The physiological/structural status of the soft ripened berry is still a matter of debate. In this paper isolated mesocarp cells from ripened berries of both wine and table varieties were studied by bright-field, fluorescence and confocal microscopy and flow cytometry to highlight the organization of berry flesh cell, function and viability. Flow cytometry analysis confirmed that protoplasting from grape berry mesocarp tissue yields a single heterogenous population of intact and viable cells. Also, the integrity of the plasma membrane and the architecture and complexity of the intracellular membranous system were shown by FM1-43 staining coupled to confocal microscopy imaging. The observed incorporation of the fluorescent glucose analogue 2-NBDG suggests that endocytosis is involved in the transport and intracellular compartmentation of apoplastic sugars. Neutral Red staining confirmed the intricate organization, size, diversity and integrity of the vacuolar apparatus that is probably related to the multifaceted roles of the vacuoles in the developing fruit. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09 2011-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/13590 |
url |
https://hdl.handle.net/1822/13590 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0002-9254 10.5344/ajev.2011.10126 The original publication is available at http://ajevonline.org/cgi/content/abstract/62/3/279 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Enology and Viticulture (ASEV) |
publisher.none.fl_str_mv |
American Society for Enology and Viticulture (ASEV) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133095206584320 |