13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy

Detalhes bibliográficos
Autor(a) principal: Burgess, Shawn C.
Data de Publicação: 2001
Outros Autores: Carvalho, Rui A., Merritt, Matthew E., Jones, John G., Malloy, Craig R., Sherry, A. Dean
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/3889
https://doi.org/10.1006/abio.2000.4930
Resumo: 13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. 1H NMR is intrinsically more sensitive than 13C NMR and can potentially supply the same information via indirect detection of 13C providing that isotopomer information can be preserved. We report here the use of J-resolved HSQC (J-HSQC) for 13C isotopomer analysis of tissue samples. We show that J-HSQC reports isotopomer multiplet patterns identical to those reported by direct 13C detection but with improved sensitivity.
id RCAP_ecc20cb2c803d870e8f40f97e8fcdcf1
oai_identifier_str oai:estudogeral.uc.pt:10316/3889
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopyindirect detection; 1H NMR; 13C isotopomer analysis; 2D NMR; HSQC13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. 1H NMR is intrinsically more sensitive than 13C NMR and can potentially supply the same information via indirect detection of 13C providing that isotopomer information can be preserved. We report here the use of J-resolved HSQC (J-HSQC) for 13C isotopomer analysis of tissue samples. We show that J-HSQC reports isotopomer multiplet patterns identical to those reported by direct 13C detection but with improved sensitivity.http://www.sciencedirect.com/science/article/B6W9V-45CWN9R-3F/1/1252248c68908a7b5bded66f146de2c62001info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/3889http://hdl.handle.net/10316/3889https://doi.org/10.1006/abio.2000.4930engAnalytical Biochemistry. 289:2 (2001) 187-195Burgess, Shawn C.Carvalho, Rui A.Merritt, Matthew E.Jones, John G.Malloy, Craig R.Sherry, A. Deaninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T13:39:34Zoai:estudogeral.uc.pt:10316/3889Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:42.326475Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
title 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
spellingShingle 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
Burgess, Shawn C.
indirect detection; 1H NMR; 13C isotopomer analysis; 2D NMR; HSQC
title_short 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
title_full 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
title_fullStr 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
title_full_unstemmed 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
title_sort 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy
author Burgess, Shawn C.
author_facet Burgess, Shawn C.
Carvalho, Rui A.
Merritt, Matthew E.
Jones, John G.
Malloy, Craig R.
Sherry, A. Dean
author_role author
author2 Carvalho, Rui A.
Merritt, Matthew E.
Jones, John G.
Malloy, Craig R.
Sherry, A. Dean
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Burgess, Shawn C.
Carvalho, Rui A.
Merritt, Matthew E.
Jones, John G.
Malloy, Craig R.
Sherry, A. Dean
dc.subject.por.fl_str_mv indirect detection; 1H NMR; 13C isotopomer analysis; 2D NMR; HSQC
topic indirect detection; 1H NMR; 13C isotopomer analysis; 2D NMR; HSQC
description 13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. 1H NMR is intrinsically more sensitive than 13C NMR and can potentially supply the same information via indirect detection of 13C providing that isotopomer information can be preserved. We report here the use of J-resolved HSQC (J-HSQC) for 13C isotopomer analysis of tissue samples. We show that J-HSQC reports isotopomer multiplet patterns identical to those reported by direct 13C detection but with improved sensitivity.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/3889
http://hdl.handle.net/10316/3889
https://doi.org/10.1006/abio.2000.4930
url http://hdl.handle.net/10316/3889
https://doi.org/10.1006/abio.2000.4930
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Analytical Biochemistry. 289:2 (2001) 187-195
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/PDF
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133843329908736

Registros relacionados