Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/70184 |
Resumo: | Problems associated with microbial resistance to antibiotics are growing due to their overuse. In this scenario, plant extracts such as the propolis extract (PE) have been considered as potential alternatives to antibiotics in the treatment of infected wounds, due to its antimicrobial properties and ability to induce tissue regeneration. To improve the long-term effectiveness of PE in wound healing, polymeric films composed of biodegradable and biocompatible polymers are being engineered as delivery vehicles. Here, sodium alginate/gelatin (SA/GN) films containing PE were prepared via a simple, green process of solvent casting/phase inversion technique, followed by crosslinking with calcium chloride (CaCl2) solutions. The minimum inhibitory concentration (MIC) of PE was established as 0.338 mg/mL for Staphylococcus aureus and 1.353 mg/mL for Pseudomonas aeruginosa, the most prevalent bacteria in infected wounds. The PE was incorporated within the polymeric films before (blended with the polymeric solution) and after (immobilization via physisorption) their production. Flexible, highly hydrated SA/GN/PE films were obtained, and their antibacterial activity was assessed via agar diffusion and killing time kinetics examinations. Data confirmed the modified films effectiveness to fight bacterial infections caused by S. aureus and P. aeruginosa and their ability to be applied in the treatment of infected wounds. |
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Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial actionAntibacterial ActivityPlant ExtractsPropolisLocalized Drug ReleaseBactericidal EffectsInfection ControlEngenharia e Tecnologia::Engenharia dos MateriaisSaúde de qualidadeProblems associated with microbial resistance to antibiotics are growing due to their overuse. In this scenario, plant extracts such as the propolis extract (PE) have been considered as potential alternatives to antibiotics in the treatment of infected wounds, due to its antimicrobial properties and ability to induce tissue regeneration. To improve the long-term effectiveness of PE in wound healing, polymeric films composed of biodegradable and biocompatible polymers are being engineered as delivery vehicles. Here, sodium alginate/gelatin (SA/GN) films containing PE were prepared via a simple, green process of solvent casting/phase inversion technique, followed by crosslinking with calcium chloride (CaCl2) solutions. The minimum inhibitory concentration (MIC) of PE was established as 0.338 mg/mL for Staphylococcus aureus and 1.353 mg/mL for Pseudomonas aeruginosa, the most prevalent bacteria in infected wounds. The PE was incorporated within the polymeric films before (blended with the polymeric solution) and after (immobilization via physisorption) their production. Flexible, highly hydrated SA/GN/PE films were obtained, and their antibacterial activity was assessed via agar diffusion and killing time kinetics examinations. Data confirmed the modified films effectiveness to fight bacterial infections caused by S. aureus and P. aeruginosa and their ability to be applied in the treatment of infected wounds.Portuguese Foundation for Science and Technol-ogy (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI), and the Portuguese Government (OE) for funding the project PEPTEX with reference PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074). The authors also acknowledge project UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTESMDPI PublishingUniversidade do MinhoHomem, Natália CândidoMiranda, Catarina S.Antunes, Joana Isabel CostaAmorim, M. T. Pessoa deFelgueiras, Helena Prado2020-112020-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70184engHomem, N. C., Miranda, C. A. F. D. S., Antunes, J. I. D. C., et. al. (2020). Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin Films with Propolis for an Improved Antimicrobial Action. In Multidisciplinary Digital Publishing Institute Proceedings (Vol. 69, No. 1, p. 4).2504-390010.3390/CGPM2020-07180https://www.mdpi.com/2504-3900/69/1/4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:31:45ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
title |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
spellingShingle |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action Homem, Natália Cândido Antibacterial Activity Plant Extracts Propolis Localized Drug Release Bactericidal Effects Infection Control Engenharia e Tecnologia::Engenharia dos Materiais Saúde de qualidade |
title_short |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
title_full |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
title_fullStr |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
title_full_unstemmed |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
title_sort |
Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin films with Propolis for an improved antimicrobial action |
author |
Homem, Natália Cândido |
author_facet |
Homem, Natália Cândido Miranda, Catarina S. Antunes, Joana Isabel Costa Amorim, M. T. Pessoa de Felgueiras, Helena Prado |
author_role |
author |
author2 |
Miranda, Catarina S. Antunes, Joana Isabel Costa Amorim, M. T. Pessoa de Felgueiras, Helena Prado |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Homem, Natália Cândido Miranda, Catarina S. Antunes, Joana Isabel Costa Amorim, M. T. Pessoa de Felgueiras, Helena Prado |
dc.subject.por.fl_str_mv |
Antibacterial Activity Plant Extracts Propolis Localized Drug Release Bactericidal Effects Infection Control Engenharia e Tecnologia::Engenharia dos Materiais Saúde de qualidade |
topic |
Antibacterial Activity Plant Extracts Propolis Localized Drug Release Bactericidal Effects Infection Control Engenharia e Tecnologia::Engenharia dos Materiais Saúde de qualidade |
description |
Problems associated with microbial resistance to antibiotics are growing due to their overuse. In this scenario, plant extracts such as the propolis extract (PE) have been considered as potential alternatives to antibiotics in the treatment of infected wounds, due to its antimicrobial properties and ability to induce tissue regeneration. To improve the long-term effectiveness of PE in wound healing, polymeric films composed of biodegradable and biocompatible polymers are being engineered as delivery vehicles. Here, sodium alginate/gelatin (SA/GN) films containing PE were prepared via a simple, green process of solvent casting/phase inversion technique, followed by crosslinking with calcium chloride (CaCl2) solutions. The minimum inhibitory concentration (MIC) of PE was established as 0.338 mg/mL for Staphylococcus aureus and 1.353 mg/mL for Pseudomonas aeruginosa, the most prevalent bacteria in infected wounds. The PE was incorporated within the polymeric films before (blended with the polymeric solution) and after (immobilization via physisorption) their production. Flexible, highly hydrated SA/GN/PE films were obtained, and their antibacterial activity was assessed via agar diffusion and killing time kinetics examinations. Data confirmed the modified films effectiveness to fight bacterial infections caused by S. aureus and P. aeruginosa and their ability to be applied in the treatment of infected wounds. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11 2020-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/70184 |
url |
http://hdl.handle.net/1822/70184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Homem, N. C., Miranda, C. A. F. D. S., Antunes, J. I. D. C., et. al. (2020). Modification of Ca2+-Crosslinked Sodium Alginate/Gelatin Films with Propolis for an Improved Antimicrobial Action. In Multidisciplinary Digital Publishing Institute Proceedings (Vol. 69, No. 1, p. 4). 2504-3900 10.3390/CGPM2020-07180 https://www.mdpi.com/2504-3900/69/1/4 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI Publishing |
publisher.none.fl_str_mv |
MDPI Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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