Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe
Autor(a) principal: | |
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Data de Publicação: | 1982 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10293 https://doi.org/10.1021/bi00266a038 |
Resumo: | Intramolecular excimer formation with 1,3-di( 1 - pyreny1)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2OOO or less) without inducing any detectable damage. The temperature was varied between -1 and 40 'C. The fluidity of the native SR membrane changes from 52 CP at 40 OC to 325 CP at 5 'C. The native SR membranes are less fluid than liposomes prepared from total membrane lipids. The fluidity of membranes reconstituted from the SR ATPase and a total lipid extract of the SR depends on the lipid/protein molar ratio and sharply decreases when this ratio becomes lower than 44, for all temperatures studied. This is in accord with literature reports describing the Occurrence of approximately 30 lipid molecules around the Ca-ATPase molecule in the SR membrane. Arrhenius plots of the excimer to monomer fluorescence intensity ratio in native and reconstituted SR membranes display a break at about 20 OC which is not observed when the lipid/ protein ratio is lower than 44. This break is interpreted as being a characteristic of the lipid portion of the membrane. |
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Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probeIntramolecular excimer formation with 1,3-di( 1 - pyreny1)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2OOO or less) without inducing any detectable damage. The temperature was varied between -1 and 40 'C. The fluidity of the native SR membrane changes from 52 CP at 40 OC to 325 CP at 5 'C. The native SR membranes are less fluid than liposomes prepared from total membrane lipids. The fluidity of membranes reconstituted from the SR ATPase and a total lipid extract of the SR depends on the lipid/protein molar ratio and sharply decreases when this ratio becomes lower than 44, for all temperatures studied. This is in accord with literature reports describing the Occurrence of approximately 30 lipid molecules around the Ca-ATPase molecule in the SR membrane. Arrhenius plots of the excimer to monomer fluorescence intensity ratio in native and reconstituted SR membranes display a break at about 20 OC which is not observed when the lipid/ protein ratio is lower than 44. This break is interpreted as being a characteristic of the lipid portion of the membrane.American Chemical Society1982-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10293http://hdl.handle.net/10316/10293https://doi.org/10.1021/bi00266a038engBiochemistry. 21:23 (1982) 5972-59770006-2960Almeida, Leonor M.Vaz, Winchil L. C.Zachariasse, Klaas A.Madeira, Vítor M. C.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-22T11:50:27Zoai:estudogeral.uc.pt:10316/10293Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:25.275323Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
title |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
spellingShingle |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe Almeida, Leonor M. |
title_short |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
title_full |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
title_fullStr |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
title_full_unstemmed |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
title_sort |
Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe |
author |
Almeida, Leonor M. |
author_facet |
Almeida, Leonor M. Vaz, Winchil L. C. Zachariasse, Klaas A. Madeira, Vítor M. C. |
author_role |
author |
author2 |
Vaz, Winchil L. C. Zachariasse, Klaas A. Madeira, Vítor M. C. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Almeida, Leonor M. Vaz, Winchil L. C. Zachariasse, Klaas A. Madeira, Vítor M. C. |
description |
Intramolecular excimer formation with 1,3-di( 1 - pyreny1)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2OOO or less) without inducing any detectable damage. The temperature was varied between -1 and 40 'C. The fluidity of the native SR membrane changes from 52 CP at 40 OC to 325 CP at 5 'C. The native SR membranes are less fluid than liposomes prepared from total membrane lipids. The fluidity of membranes reconstituted from the SR ATPase and a total lipid extract of the SR depends on the lipid/protein molar ratio and sharply decreases when this ratio becomes lower than 44, for all temperatures studied. This is in accord with literature reports describing the Occurrence of approximately 30 lipid molecules around the Ca-ATPase molecule in the SR membrane. Arrhenius plots of the excimer to monomer fluorescence intensity ratio in native and reconstituted SR membranes display a break at about 20 OC which is not observed when the lipid/ protein ratio is lower than 44. This break is interpreted as being a characteristic of the lipid portion of the membrane. |
publishDate |
1982 |
dc.date.none.fl_str_mv |
1982-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10293 http://hdl.handle.net/10316/10293 https://doi.org/10.1021/bi00266a038 |
url |
http://hdl.handle.net/10316/10293 https://doi.org/10.1021/bi00266a038 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemistry. 21:23 (1982) 5972-5977 0006-2960 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133841618632704 |