Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5859 https://doi.org/10.1016/j.ejps.2006.12.007 |
Resumo: | Insulin-loaded alginate-dextran nanospheres were prepared by nanoemulsion dispersion followed by triggered in situ gelation. Nanospheres were characterized for mean size and distribution by laser diffraction spectroscopy and for shape by transmission electron microscopy. Insulin encapsulation efficiency and in vitro release were determined by Bradford protein assay and bioactivity determined in vitro using a newly developed Western blot immunoassay and in vivo using Wistar diabetic rats. Nanospheres ranged from 267 nm to 2.76 [mu]m in diameter and demonstrated a unimodal size distribution. Insulin encapsulation efficiency was 82.5%. Alginate-dextran particles suppressed insulin release in acidic media and promoted a sustained release at near neutral conditions. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro bioassays |
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Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivityAlginate-dextran nanospheresNanospheresInsulin bioactivityInsulin-loaded alginate-dextran nanospheres were prepared by nanoemulsion dispersion followed by triggered in situ gelation. Nanospheres were characterized for mean size and distribution by laser diffraction spectroscopy and for shape by transmission electron microscopy. Insulin encapsulation efficiency and in vitro release were determined by Bradford protein assay and bioactivity determined in vitro using a newly developed Western blot immunoassay and in vivo using Wistar diabetic rats. Nanospheres ranged from 267 nm to 2.76 [mu]m in diameter and demonstrated a unimodal size distribution. Insulin encapsulation efficiency was 82.5%. Alginate-dextran particles suppressed insulin release in acidic media and promoted a sustained release at near neutral conditions. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro bioassayshttp://www.sciencedirect.com/science/article/B6T25-4MTC6GD-1/1/c26097e450ad242243e8df9feae8643f2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5859http://hdl.handle.net/10316/5859https://doi.org/10.1016/j.ejps.2006.12.007engEuropean Journal of Pharmaceutical Sciences. 30:5 (2007) 392-397Reis, Catarina PintoRibeiro, António J.Houng, SimoneVeiga, FranciscoNeufeld, Ronald J.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:49:01Zoai:estudogeral.uc.pt:10316/5859Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:19.772096Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
title |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
spellingShingle |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity Reis, Catarina Pinto Alginate-dextran nanospheres Nanospheres Insulin bioactivity |
title_short |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
title_full |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
title_fullStr |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
title_full_unstemmed |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
title_sort |
Nanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivity |
author |
Reis, Catarina Pinto |
author_facet |
Reis, Catarina Pinto Ribeiro, António J. Houng, Simone Veiga, Francisco Neufeld, Ronald J. |
author_role |
author |
author2 |
Ribeiro, António J. Houng, Simone Veiga, Francisco Neufeld, Ronald J. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Reis, Catarina Pinto Ribeiro, António J. Houng, Simone Veiga, Francisco Neufeld, Ronald J. |
dc.subject.por.fl_str_mv |
Alginate-dextran nanospheres Nanospheres Insulin bioactivity |
topic |
Alginate-dextran nanospheres Nanospheres Insulin bioactivity |
description |
Insulin-loaded alginate-dextran nanospheres were prepared by nanoemulsion dispersion followed by triggered in situ gelation. Nanospheres were characterized for mean size and distribution by laser diffraction spectroscopy and for shape by transmission electron microscopy. Insulin encapsulation efficiency and in vitro release were determined by Bradford protein assay and bioactivity determined in vitro using a newly developed Western blot immunoassay and in vivo using Wistar diabetic rats. Nanospheres ranged from 267 nm to 2.76 [mu]m in diameter and demonstrated a unimodal size distribution. Insulin encapsulation efficiency was 82.5%. Alginate-dextran particles suppressed insulin release in acidic media and promoted a sustained release at near neutral conditions. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro bioassays |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5859 http://hdl.handle.net/10316/5859 https://doi.org/10.1016/j.ejps.2006.12.007 |
url |
http://hdl.handle.net/10316/5859 https://doi.org/10.1016/j.ejps.2006.12.007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Pharmaceutical Sciences. 30:5 (2007) 392-397 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133750586507264 |