Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/18753 |
Resumo: | Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16−/− without treatment, group II: treated HPV16−/−, group III: HPV16+/− without treatment and group IV: treated HPV16+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wildtype animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers. |
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Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic miceCancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16−/− without treatment, group II: treated HPV16−/−, group III: HPV16+/− without treatment and group IV: treated HPV16+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wildtype animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers.This work was supported by: Integrative Research in Environment, Agro-Chains and Technology no. NORTE-01- 0145-FEDER-000017, in its line of research entitled ISAC, cofinanced by the European Regional Development Fund (ERDF) through NORTE 2020 (North Regional Operational Program 2014/2020). European Investment Funds by FEDER/COMPETE/ POCI– Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013. This study was also funded by Liga Portuguesa Contra o Cancro, by the Research Center of the Portuguese Institute of Oncology of Porto (CI-IPOP 37-2016), by project POCI-01-0145- FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE), project POCI-01-0145-FEDER-006958 and UID/AGR/04033/2013, funded by FEDER funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT – Fundação para a Ciência e a Tecnologia; Rui M. Gil da Costa was funded by grant number SFRH/BPD/85462/2012 from FCT, funded by the Portuguese Government and the Social European Fund. The authors are also grateful to FCT, Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/ 00690/2013), and to the Interreg España-Portugal for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBMedeiros-Fonseca, BeatrizMestre, V.F.Colaço, B.Pires, Maria JoãoMartins, TâniaCosta, Rui Miguel GilNeuparth, Maria JoãoMedeiros, RuiMoutinho, Magda S.S.Dias, Maria InêsBarros, LillianBastos, Margarida M.S.M.Félix, LuísVenâncio, CarlosFerreira, Isabel C.F.R.Antunes, L.M.Oliveira, Paula A.2018-01-19T10:00:00Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/18753engMedeiros-Fonseca, B.; Mestre, V. F.; Colaço, B.; Pires, M.J.; Martins, T.; Costa, R.M. Gil; Neuparth, M.J.; Medeiros, R.; Moutinho, Magda S.S.; Dias, Maria Inês; Barros, Lillian; Bastos, M.M.S.M.; Félix, L.; Venâncio, C.; Ferreira, Isabel C.F.R.; Antunes, L.M.; Oliveira, P.A. (2018). Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice. Food and Function. ISSN 2042-6496. 9, p. 4419-44282042-649610.1039/c8fo00783ginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-01T01:18:11ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
title |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
spellingShingle |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice Medeiros-Fonseca, Beatriz |
title_short |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
title_full |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
title_fullStr |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
title_full_unstemmed |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
title_sort |
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
author |
Medeiros-Fonseca, Beatriz |
author_facet |
Medeiros-Fonseca, Beatriz Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
author_role |
author |
author2 |
Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Medeiros-Fonseca, Beatriz Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
description |
Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16−/− without treatment, group II: treated HPV16−/−, group III: HPV16+/− without treatment and group IV: treated HPV16+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wildtype animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/18753 |
url |
http://hdl.handle.net/10198/18753 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medeiros-Fonseca, B.; Mestre, V. F.; Colaço, B.; Pires, M.J.; Martins, T.; Costa, R.M. Gil; Neuparth, M.J.; Medeiros, R.; Moutinho, Magda S.S.; Dias, Maria Inês; Barros, Lillian; Bastos, M.M.S.M.; Félix, L.; Venâncio, C.; Ferreira, Isabel C.F.R.; Antunes, L.M.; Oliveira, P.A. (2018). Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice. Food and Function. ISSN 2042-6496. 9, p. 4419-4428 2042-6496 10.1039/c8fo00783g |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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