Highly polymorphic microsatellite for identification of Candida albicans strains
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/2245 |
Resumo: | The polymorphism of a new microsatellite locus (CAI) was investigated in a total of 114 C. albicans strains, including 73 independent clinical isolates, multiple isolates from the same patient, isolates from several episodes of recurrent vulvovaginal infections and two reference strains. PCR genotyping was performed automatically, using a fluorescent labelled primer and, in the 73 independent isolates, 26 alleles and 44 different genotypes were identified, resulting in a discriminatory power of 0.97. CAI revealed to be species specific and with a low mutation rate, since no amplification product was obtained when testing other pathogenic Candida species and no genotype differences were observed when testing over 300 generations. When applying this microsatellite to the identification of strains isolated from recurrent vulvovaginal infections in eight patients, it was found that 13 out of 15 episodes were due to the same strain. When multiple isolates obtained from the same patient and plated simultaneously, were typed for CAI, the same genotype was found in each case, confirming that the infecting population was clonal. Moreover, the same genotype appeared in isolates from the rectus and the vagina, revealing that the former could be a reservoir of potentially virulent strains. This new microsatellite proves to be a valuable tool to differentiate C. albicans strains. Furthermore, when compared to other molecular genotyping techniques, CAI proved to be very simple, highly efficient and reproducible, being suitable for low quantity and very degraded samples and for application in large scale epidemiological studies. |
id |
RCAP_f03df84957400f2935ce190e43070226 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/2245 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
|
spelling |
Highly polymorphic microsatellite for identification of Candida albicans strainsMicrosatelliteCandida albicansScience & TechnologyThe polymorphism of a new microsatellite locus (CAI) was investigated in a total of 114 C. albicans strains, including 73 independent clinical isolates, multiple isolates from the same patient, isolates from several episodes of recurrent vulvovaginal infections and two reference strains. PCR genotyping was performed automatically, using a fluorescent labelled primer and, in the 73 independent isolates, 26 alleles and 44 different genotypes were identified, resulting in a discriminatory power of 0.97. CAI revealed to be species specific and with a low mutation rate, since no amplification product was obtained when testing other pathogenic Candida species and no genotype differences were observed when testing over 300 generations. When applying this microsatellite to the identification of strains isolated from recurrent vulvovaginal infections in eight patients, it was found that 13 out of 15 episodes were due to the same strain. When multiple isolates obtained from the same patient and plated simultaneously, were typed for CAI, the same genotype was found in each case, confirming that the infecting population was clonal. Moreover, the same genotype appeared in isolates from the rectus and the vagina, revealing that the former could be a reservoir of potentially virulent strains. This new microsatellite proves to be a valuable tool to differentiate C. albicans strains. Furthermore, when compared to other molecular genotyping techniques, CAI proved to be very simple, highly efficient and reproducible, being suitable for low quantity and very degraded samples and for application in large scale epidemiological studies.Fundação para a Ciência e a Tecnologia (FCT) through a pluriannual contract with Centro de Ciências do Ambiente (CCA), Universidade do Minho.American Society for Microbiology (ASM)Universidade do MinhoSampaio, PaulaGusmão, LeonorAlves, CintiaVaz, Cidália PinaAmorim, AntónioPais, Célia2003-022003-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2245eng"Journal of clinical microbiology". ISSN 1098-660X. 41 (2003) 552-557.1098-660X10.1128/JCM.41.2.552-557.200312574245info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:33ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Highly polymorphic microsatellite for identification of Candida albicans strains |
title |
Highly polymorphic microsatellite for identification of Candida albicans strains |
spellingShingle |
Highly polymorphic microsatellite for identification of Candida albicans strains Sampaio, Paula Microsatellite Candida albicans Science & Technology |
title_short |
Highly polymorphic microsatellite for identification of Candida albicans strains |
title_full |
Highly polymorphic microsatellite for identification of Candida albicans strains |
title_fullStr |
Highly polymorphic microsatellite for identification of Candida albicans strains |
title_full_unstemmed |
Highly polymorphic microsatellite for identification of Candida albicans strains |
title_sort |
Highly polymorphic microsatellite for identification of Candida albicans strains |
author |
Sampaio, Paula |
author_facet |
Sampaio, Paula Gusmão, Leonor Alves, Cintia Vaz, Cidália Pina Amorim, António Pais, Célia |
author_role |
author |
author2 |
Gusmão, Leonor Alves, Cintia Vaz, Cidália Pina Amorim, António Pais, Célia |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sampaio, Paula Gusmão, Leonor Alves, Cintia Vaz, Cidália Pina Amorim, António Pais, Célia |
dc.subject.por.fl_str_mv |
Microsatellite Candida albicans Science & Technology |
topic |
Microsatellite Candida albicans Science & Technology |
description |
The polymorphism of a new microsatellite locus (CAI) was investigated in a total of 114 C. albicans strains, including 73 independent clinical isolates, multiple isolates from the same patient, isolates from several episodes of recurrent vulvovaginal infections and two reference strains. PCR genotyping was performed automatically, using a fluorescent labelled primer and, in the 73 independent isolates, 26 alleles and 44 different genotypes were identified, resulting in a discriminatory power of 0.97. CAI revealed to be species specific and with a low mutation rate, since no amplification product was obtained when testing other pathogenic Candida species and no genotype differences were observed when testing over 300 generations. When applying this microsatellite to the identification of strains isolated from recurrent vulvovaginal infections in eight patients, it was found that 13 out of 15 episodes were due to the same strain. When multiple isolates obtained from the same patient and plated simultaneously, were typed for CAI, the same genotype was found in each case, confirming that the infecting population was clonal. Moreover, the same genotype appeared in isolates from the rectus and the vagina, revealing that the former could be a reservoir of potentially virulent strains. This new microsatellite proves to be a valuable tool to differentiate C. albicans strains. Furthermore, when compared to other molecular genotyping techniques, CAI proved to be very simple, highly efficient and reproducible, being suitable for low quantity and very degraded samples and for application in large scale epidemiological studies. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-02 2003-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/2245 |
url |
http://hdl.handle.net/1822/2245 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of clinical microbiology". ISSN 1098-660X. 41 (2003) 552-557. 1098-660X 10.1128/JCM.41.2.552-557.2003 12574245 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1777303861044707328 |