Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

Detalhes bibliográficos
Autor(a) principal: Valentão, Patrı́cia
Data de Publicação: 2004
Outros Autores: Carvalho, Márcia, Fernandes, Eduarda, Carvalho, Félix, Andrade, Paula Branquinho, Seabra, Rosa Maria, Bastos, Maria de Lourdes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10284/10009
Resumo: Hypericum androsaemum L. (Guttiferae) is a medicinal plant with antioxidant activity. Increasing evidence regarding free radical generating agents suggests that hepatotoxic-related disorders may involve reactive oxygen species (ROS). The purpose of this study was to investigate the protective effect of Hypericum androsaemum infusion on isolated rat hepatocytes oxidative injury induced by tert-butyl hydroperoxide (t-BHP). The results showed that pretreatment of the cells with this infusion (16, 62 and 250 microg/ml) prevented the leakage of lactate dehydrogenase (LDH) and lipid peroxidation caused by a 30-min treatment with t-BHP (1mM). However, infusion-induced alterations on glutathione homeostasis were noticed, as it was observed by the increase in glutathione oxidised form (GSSG) and depletion in total glutathione levels, which indicates that plant-derived antioxidant extracts may not be considered a generalised way of treating pro-oxidant-related diseases.
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spelling Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytesHypericum androsaemumIsolated hepatocytesTert-Butyl hydroperoxideHepatoprotective activityHypericum androsaemum L. (Guttiferae) is a medicinal plant with antioxidant activity. Increasing evidence regarding free radical generating agents suggests that hepatotoxic-related disorders may involve reactive oxygen species (ROS). The purpose of this study was to investigate the protective effect of Hypericum androsaemum infusion on isolated rat hepatocytes oxidative injury induced by tert-butyl hydroperoxide (t-BHP). The results showed that pretreatment of the cells with this infusion (16, 62 and 250 microg/ml) prevented the leakage of lactate dehydrogenase (LDH) and lipid peroxidation caused by a 30-min treatment with t-BHP (1mM). However, infusion-induced alterations on glutathione homeostasis were noticed, as it was observed by the increase in glutathione oxidised form (GSSG) and depletion in total glutathione levels, which indicates that plant-derived antioxidant extracts may not be considered a generalised way of treating pro-oxidant-related diseases.ElsevierRepositório Institucional da Universidade Fernando PessoaValentão, Patrı́ciaCarvalho, MárciaFernandes, EduardaCarvalho, FélixAndrade, Paula BranquinhoSeabra, Rosa MariaBastos, Maria de Lourdes2021-07-02T08:54:46Z2004-01-01T00:00:00Z2004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10284/10009eng0378-874110.1016/j.jep.2004.02.004metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T02:09:18ZPortal AgregadorONG
dc.title.none.fl_str_mv Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
title Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
spellingShingle Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
Valentão, Patrı́cia
Hypericum androsaemum
Isolated hepatocytes
Tert-Butyl hydroperoxide
Hepatoprotective activity
title_short Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
title_full Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
title_fullStr Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
title_full_unstemmed Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
title_sort Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes
author Valentão, Patrı́cia
author_facet Valentão, Patrı́cia
Carvalho, Márcia
Fernandes, Eduarda
Carvalho, Félix
Andrade, Paula Branquinho
Seabra, Rosa Maria
Bastos, Maria de Lourdes
author_role author
author2 Carvalho, Márcia
Fernandes, Eduarda
Carvalho, Félix
Andrade, Paula Branquinho
Seabra, Rosa Maria
Bastos, Maria de Lourdes
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Institucional da Universidade Fernando Pessoa
dc.contributor.author.fl_str_mv Valentão, Patrı́cia
Carvalho, Márcia
Fernandes, Eduarda
Carvalho, Félix
Andrade, Paula Branquinho
Seabra, Rosa Maria
Bastos, Maria de Lourdes
dc.subject.por.fl_str_mv Hypericum androsaemum
Isolated hepatocytes
Tert-Butyl hydroperoxide
Hepatoprotective activity
topic Hypericum androsaemum
Isolated hepatocytes
Tert-Butyl hydroperoxide
Hepatoprotective activity
description Hypericum androsaemum L. (Guttiferae) is a medicinal plant with antioxidant activity. Increasing evidence regarding free radical generating agents suggests that hepatotoxic-related disorders may involve reactive oxygen species (ROS). The purpose of this study was to investigate the protective effect of Hypericum androsaemum infusion on isolated rat hepatocytes oxidative injury induced by tert-butyl hydroperoxide (t-BHP). The results showed that pretreatment of the cells with this infusion (16, 62 and 250 microg/ml) prevented the leakage of lactate dehydrogenase (LDH) and lipid peroxidation caused by a 30-min treatment with t-BHP (1mM). However, infusion-induced alterations on glutathione homeostasis were noticed, as it was observed by the increase in glutathione oxidised form (GSSG) and depletion in total glutathione levels, which indicates that plant-derived antioxidant extracts may not be considered a generalised way of treating pro-oxidant-related diseases.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01T00:00:00Z
2004-01-01T00:00:00Z
2021-07-02T08:54:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10284/10009
url http://hdl.handle.net/10284/10009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0378-8741
10.1016/j.jep.2004.02.004
dc.rights.driver.fl_str_mv metadata only access
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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