Boosting antibiotics performance by new formulations with deep eutectic solvents
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/36058 |
Resumo: | The critical scenario of antimicrobial resistance to antibiotics highlights the need for improved therapeutics and/or formulations. Herein, we demonstrate that deep eutectic solvents (DES) formulations are very promising to remarkably improve the solubility, stability and therapeutic efficacy of antibiotics, such as ciprofloxacin. DES aqueous solutions enhance the solubility of ciprofloxacin up to 430-fold while extending the antibiotic stability. The developed formulations can improve, by 2 to 4-fold, the susceptibility of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria to the antibiotic. They also improve the therapeutic efficacy at concentrations where bacteria present resistance, without promoting tolerance development to ciprofloxacin. Furthermore, the incorporation of DES decreases the toxicity of ciprofloxacin towards immortalized human epidermal keratinocytes (HaCat cells). The results herein reveal the pioneering use of DES in fluoroquinolone-based formulations and their impact on the antibiotic's characteristics and on its therapeutic action. |
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Boosting antibiotics performance by new formulations with deep eutectic solventsCiprofloxacinHumansMicrobial Sensitivity TestsSolventsStaphylococcus aureusAnti-Bacterial AgentsDeep Eutectic SolventsThe critical scenario of antimicrobial resistance to antibiotics highlights the need for improved therapeutics and/or formulations. Herein, we demonstrate that deep eutectic solvents (DES) formulations are very promising to remarkably improve the solubility, stability and therapeutic efficacy of antibiotics, such as ciprofloxacin. DES aqueous solutions enhance the solubility of ciprofloxacin up to 430-fold while extending the antibiotic stability. The developed formulations can improve, by 2 to 4-fold, the susceptibility of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria to the antibiotic. They also improve the therapeutic efficacy at concentrations where bacteria present resistance, without promoting tolerance development to ciprofloxacin. Furthermore, the incorporation of DES decreases the toxicity of ciprofloxacin towards immortalized human epidermal keratinocytes (HaCat cells). The results herein reveal the pioneering use of DES in fluoroquinolone-based formulations and their impact on the antibiotic's characteristics and on its therapeutic action.Elsevier2023-01-27T10:51:08Z2022-03-25T00:00:00Z2022-03-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/36058eng0378-517310.1016/j.ijpharm.2022.121566Pedro, Sónia N.Gomes, Ana T. P. C.Oskoei, PárástuOliveira, HelenaAlmeida, AdelaideFreire, Mara G.Silvestre, Armando J. D.Freire, Carmen S. R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:08:52Zoai:ria.ua.pt:10773/36058Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:06:42.341774Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
title |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
spellingShingle |
Boosting antibiotics performance by new formulations with deep eutectic solvents Pedro, Sónia N. Ciprofloxacin Humans Microbial Sensitivity Tests Solvents Staphylococcus aureus Anti-Bacterial Agents Deep Eutectic Solvents |
title_short |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
title_full |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
title_fullStr |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
title_full_unstemmed |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
title_sort |
Boosting antibiotics performance by new formulations with deep eutectic solvents |
author |
Pedro, Sónia N. |
author_facet |
Pedro, Sónia N. Gomes, Ana T. P. C. Oskoei, Párástu Oliveira, Helena Almeida, Adelaide Freire, Mara G. Silvestre, Armando J. D. Freire, Carmen S. R. |
author_role |
author |
author2 |
Gomes, Ana T. P. C. Oskoei, Párástu Oliveira, Helena Almeida, Adelaide Freire, Mara G. Silvestre, Armando J. D. Freire, Carmen S. R. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pedro, Sónia N. Gomes, Ana T. P. C. Oskoei, Párástu Oliveira, Helena Almeida, Adelaide Freire, Mara G. Silvestre, Armando J. D. Freire, Carmen S. R. |
dc.subject.por.fl_str_mv |
Ciprofloxacin Humans Microbial Sensitivity Tests Solvents Staphylococcus aureus Anti-Bacterial Agents Deep Eutectic Solvents |
topic |
Ciprofloxacin Humans Microbial Sensitivity Tests Solvents Staphylococcus aureus Anti-Bacterial Agents Deep Eutectic Solvents |
description |
The critical scenario of antimicrobial resistance to antibiotics highlights the need for improved therapeutics and/or formulations. Herein, we demonstrate that deep eutectic solvents (DES) formulations are very promising to remarkably improve the solubility, stability and therapeutic efficacy of antibiotics, such as ciprofloxacin. DES aqueous solutions enhance the solubility of ciprofloxacin up to 430-fold while extending the antibiotic stability. The developed formulations can improve, by 2 to 4-fold, the susceptibility of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria to the antibiotic. They also improve the therapeutic efficacy at concentrations where bacteria present resistance, without promoting tolerance development to ciprofloxacin. Furthermore, the incorporation of DES decreases the toxicity of ciprofloxacin towards immortalized human epidermal keratinocytes (HaCat cells). The results herein reveal the pioneering use of DES in fluoroquinolone-based formulations and their impact on the antibiotic's characteristics and on its therapeutic action. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-25T00:00:00Z 2022-03-25 2023-01-27T10:51:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/36058 |
url |
http://hdl.handle.net/10773/36058 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0378-5173 10.1016/j.ijpharm.2022.121566 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137722500120576 |