Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/6409 |
Resumo: | Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methodsOccupational exposureFungal exposureMolecular methodsPCRSuberosisPortugalCork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry.De GruyterRCIPLViegas, CarlaSabino, RaquelBotelho, DanielSantos, Mateus dosGomes, Anita Quintal2016-08-25T10:52:27Z2015-092015-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/6409engViegas C, Sabino R, Botelho D, dos Santos M, Gomes AQ. Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods. Arh Hig Rada Toksikol. 2015;66(3):203-7.10.1515/aiht-2015-66-2614info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:51:09Zoai:repositorio.ipl.pt:10400.21/6409Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:15:32.792268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
spellingShingle |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods Viegas, Carla Occupational exposure Fungal exposure Molecular methods PCR Suberosis Portugal |
title_short |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_full |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_fullStr |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_full_unstemmed |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_sort |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
author |
Viegas, Carla |
author_facet |
Viegas, Carla Sabino, Raquel Botelho, Daniel Santos, Mateus dos Gomes, Anita Quintal |
author_role |
author |
author2 |
Sabino, Raquel Botelho, Daniel Santos, Mateus dos Gomes, Anita Quintal |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Viegas, Carla Sabino, Raquel Botelho, Daniel Santos, Mateus dos Gomes, Anita Quintal |
dc.subject.por.fl_str_mv |
Occupational exposure Fungal exposure Molecular methods PCR Suberosis Portugal |
topic |
Occupational exposure Fungal exposure Molecular methods PCR Suberosis Portugal |
description |
Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09 2015-09-01T00:00:00Z 2016-08-25T10:52:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/6409 |
url |
http://hdl.handle.net/10400.21/6409 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Viegas C, Sabino R, Botelho D, dos Santos M, Gomes AQ. Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods. Arh Hig Rada Toksikol. 2015;66(3):203-7. 10.1515/aiht-2015-66-2614 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
De Gruyter |
publisher.none.fl_str_mv |
De Gruyter |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133414118391808 |