Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/23664 |
Resumo: | First published online: 2012 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogelsGellan GumGlycidyl methacrylateHigh-acyl gellan gum (HA-GG)HydrogelNucleus pulposusScience & TechnologyFirst published online: 2012Gellan gum (GG)-based hydrogels are advantageous in tissue engineering not only due to their ability to retain large quantities of water and provide a similar environment to that of natural extracellular matrix (ECM), but also because they can gelify in situ in seconds. Their mechanical properties can be fine-tuned to mimic natural tissues such as the nucleus pulposus (NP). This study produced different formulations of GG hydrogels by mixing varying amounts of methacrylated (GG-MA) and high-acyl gellan gums (HA-GG) for applications as acellular and cellular NP substitutes. The hydrogels were physicochemically characterized by dynamic mechanical analysis. Degradation and swelling abilities were assessed by soaking in a phosphate buffered saline solution for up to 170 h. Results showed that as HA-GG content increased, the modulus of the hydrogels decreased. Moreover, increases in HA-GG content induced greater weight loss in the GG-MA/HA-GG formulation compared to GG-MA hydrogel. Potential cytotoxicity of the hydrogel was assessed by culturing rabbit NP cells up to 7 days. An MTS assay was performed by seeding rabbit NP cells onto the surface of 3D hydrogel disc formulations. Viability of rabbit NP cells encapsulated within the different hydrogel formulations was also evaluated by Calcein-AM and ATP assays. Results showed that tunable GG-MA/HA-GG hydrogels were non-cytotoxic and supported viability of rabbit NP cells.The authors thank the Portuguese Foundation for Science and Technology (FCT) for financial support through POCTI and FEDER programmes. This work was also conducted with the support of the European Union funded Collaborative Project Disc Regeneration (NMP3-LA-2008-213904), MBC(A04003) and the World Class University project (R31-20029).WileyUniversidade do MinhoKhang, G.Lee, S. K.Kim, H. N.Silva-Correia, JoanaGomes, Manuela E.Viegas, C. A. A.Dias, Isabel R.Oliveira, Joaquim M.Reis, R. L.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23664engKhang, G., Lee, S. K., Kim, H. N., Silva-Correia, J., Gomes, M. E., Viegas, C. A. A., . . . Reis, R. L. (2015). Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels. Journal of Tissue Engineering and Regenerative Medicine, 9(3), 265-275. doi: 10.1002/term.16251932-625410.1002/term.162523225767http://onlinelibrary.wiley.com/doi/10.1002/term.1625/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:32Zoai:repositorium.sdum.uminho.pt:1822/23664Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:24.245744Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| title |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| spellingShingle |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels Khang, G. Gellan Gum Glycidyl methacrylate High-acyl gellan gum (HA-GG) Hydrogel Nucleus pulposus Science & Technology |
| title_short |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| title_full |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| title_fullStr |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| title_full_unstemmed |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| title_sort |
Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels |
| author |
Khang, G. |
| author_facet |
Khang, G. Lee, S. K. Kim, H. N. Silva-Correia, Joana Gomes, Manuela E. Viegas, C. A. A. Dias, Isabel R. Oliveira, Joaquim M. Reis, R. L. |
| author_role |
author |
| author2 |
Lee, S. K. Kim, H. N. Silva-Correia, Joana Gomes, Manuela E. Viegas, C. A. A. Dias, Isabel R. Oliveira, Joaquim M. Reis, R. L. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Khang, G. Lee, S. K. Kim, H. N. Silva-Correia, Joana Gomes, Manuela E. Viegas, C. A. A. Dias, Isabel R. Oliveira, Joaquim M. Reis, R. L. |
| dc.subject.por.fl_str_mv |
Gellan Gum Glycidyl methacrylate High-acyl gellan gum (HA-GG) Hydrogel Nucleus pulposus Science & Technology |
| topic |
Gellan Gum Glycidyl methacrylate High-acyl gellan gum (HA-GG) Hydrogel Nucleus pulposus Science & Technology |
| description |
First published online: 2012 |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/23664 |
| url |
http://hdl.handle.net/1822/23664 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Khang, G., Lee, S. K., Kim, H. N., Silva-Correia, J., Gomes, M. E., Viegas, C. A. A., . . . Reis, R. L. (2015). Biological evaluation of intervertebral disc cells in different formulations of gellan gum-based hydrogels. Journal of Tissue Engineering and Regenerative Medicine, 9(3), 265-275. doi: 10.1002/term.1625 1932-6254 10.1002/term.1625 23225767 http://onlinelibrary.wiley.com/doi/10.1002/term.1625/abstract |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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