Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
| Autor(a) principal: | |
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| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/11304 |
Resumo: | Copyright © 2011, American Society for Microbiology. All Rights Reserved. |
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Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patientsCopyright © 2011, American Society for Microbiology. All Rights Reserved.Viremia is significantly lower in HIV-2 than in HIV-1 infection, irrespective of disease stage. Nevertheless, the comparable proviral DNA burdens observed for these two infections indicate similar numbers of infected cells. Here we investigated this apparent paradox by assessing cell-associated viral replication. We found that untreated HIV-1-positive (HIV-1(+)) and HIV-2(+) individuals, matched for CD4 T cell depletion, exhibited similar gag mRNA levels, indicating that significant viral transcription is occurring in untreated HIV-2(+) patients, despite the reduced viremia (undetectable to 2.6 × 10(4) RNA copies/ml). However, tat mRNA transcripts were observed at significantly lower levels in HIV-2(+) patients, suggesting that the rate of de novo infection is decreased in these patients. Our data also reveal a direct relationship of gag and tat transcripts with CD4 and CD8 T cell activation, respectively. Antiretroviral therapy (ART)-treated HIV-2(+) patients showed persistent viral replication, irrespective of plasma viremia, possibly contributing to the emergence of drug resistance mutations, persistent hyperimmune activation, and poor CD4 T cell recovery that we observed with these individuals. In conclusion, we provide here evidence of significant ongoing viral replication in HIV-2(+) patients, further emphasizing the dichotomy between amount of plasma virus and cell-associated viral burden and stressing the need for antiretroviral trials and the definition of therapeutic guidelines for HIV-2 infection.This study was funded by grants from Fundação para a Ciência e a Tecnologia (FCT) and Programa Operacional Ciência e Inovação 2010 (POCI2010), as well as from Fundação Calouste Gulbenkian, to A.E.S. A.P.B. received a scholarship from GlaxoSmithKline, and R.S.S., R.T., R.B.F., and R.C. a scholarship from FCT.American Society for MicrobiologyRepositório da Universidade de LisboaSoares, Rui S.Tendeiro, RitaFoxall, Russell B.Baptista, António P.Cavaleiro, RitaGomes, PerpétuaCamacho, RicardoValadas, EmíliaDoroana, ManuelaLucas, MargaridaAntunes, FranciscoVictorino, Rui M. M.Sousa, Ana E.2014-06-19T15:13:36Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/11304engJournal of Virology Mar. 2011, p. 2429–24380022-538Xhttp://jvi.asm.org/content/85/5/2429info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-14T15:07:44ZPortal AgregadorONG |
| dc.title.none.fl_str_mv |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| title |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| spellingShingle |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients Soares, Rui S. |
| title_short |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| title_full |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| title_fullStr |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| title_full_unstemmed |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| title_sort |
Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients |
| author |
Soares, Rui S. |
| author_facet |
Soares, Rui S. Tendeiro, Rita Foxall, Russell B. Baptista, António P. Cavaleiro, Rita Gomes, Perpétua Camacho, Ricardo Valadas, Emília Doroana, Manuela Lucas, Margarida Antunes, Francisco Victorino, Rui M. M. Sousa, Ana E. |
| author_role |
author |
| author2 |
Tendeiro, Rita Foxall, Russell B. Baptista, António P. Cavaleiro, Rita Gomes, Perpétua Camacho, Ricardo Valadas, Emília Doroana, Manuela Lucas, Margarida Antunes, Francisco Victorino, Rui M. M. Sousa, Ana E. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Soares, Rui S. Tendeiro, Rita Foxall, Russell B. Baptista, António P. Cavaleiro, Rita Gomes, Perpétua Camacho, Ricardo Valadas, Emília Doroana, Manuela Lucas, Margarida Antunes, Francisco Victorino, Rui M. M. Sousa, Ana E. |
| description |
Copyright © 2011, American Society for Microbiology. All Rights Reserved. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2014-06-19T15:13:36Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/11304 |
| url |
http://hdl.handle.net/10451/11304 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Virology Mar. 2011, p. 2429–2438 0022-538X http://jvi.asm.org/content/85/5/2429 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303284983267328 |