Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography

Detalhes bibliográficos
Autor(a) principal: Santos, Maria João
Data de Publicação: 2012
Outros Autores: Teixeira, J. A., Rodrigues, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/19933
Resumo: A method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl). The proposed method was found to be suitable to fractionate the major whey proteins from the WPC80 in different fractions, namely one fraction containing all the a-Lactalbumin and immunoglobulins; another fraction containing all the bovine serum albumin; and two distinct fractions each containing a different variant of b-Lactoglobulin. A 60.5% (w/w) recovery of the two main b-Lactoglobulin variants was obtained.
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spelling Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatographyWhey proteinsAnion-exchange chromatographySeparationIsolationScience & TechnologyA method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl). The proposed method was found to be suitable to fractionate the major whey proteins from the WPC80 in different fractions, namely one fraction containing all the a-Lactalbumin and immunoglobulins; another fraction containing all the bovine serum albumin; and two distinct fractions each containing a different variant of b-Lactoglobulin. A 60.5% (w/w) recovery of the two main b-Lactoglobulin variants was obtained.ElsevierUniversidade do MinhoSantos, Maria JoãoTeixeira, J. A.Rodrigues, L. R.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/19933eng1383-586610.1016/j.seppur.2012.02.030info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:50Zoai:repositorium.sdum.uminho.pt:1822/19933Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:39.683329Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
title Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
spellingShingle Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
Santos, Maria João
Whey proteins
Anion-exchange chromatography
Separation
Isolation
Science & Technology
title_short Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
title_full Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
title_fullStr Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
title_full_unstemmed Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
title_sort Fractionation of the major whey proteins and isolation of β-Lactoglobulin variants by anion exchange chromatography
author Santos, Maria João
author_facet Santos, Maria João
Teixeira, J. A.
Rodrigues, L. R.
author_role author
author2 Teixeira, J. A.
Rodrigues, L. R.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Maria João
Teixeira, J. A.
Rodrigues, L. R.
dc.subject.por.fl_str_mv Whey proteins
Anion-exchange chromatography
Separation
Isolation
Science & Technology
topic Whey proteins
Anion-exchange chromatography
Separation
Isolation
Science & Technology
description A method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl). The proposed method was found to be suitable to fractionate the major whey proteins from the WPC80 in different fractions, namely one fraction containing all the a-Lactalbumin and immunoglobulins; another fraction containing all the bovine serum albumin; and two distinct fractions each containing a different variant of b-Lactoglobulin. A 60.5% (w/w) recovery of the two main b-Lactoglobulin variants was obtained.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/19933
url http://hdl.handle.net/1822/19933
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1383-5866
10.1016/j.seppur.2012.02.030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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