Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2022
Outros Autores: Cervantes, Renata, Dias, Marta, Gomes, Bianca, Pena, Pedro, Carolino, Elisabete, Twarużek, Magdalena, Kosicki, Robert, Soszczyńska, Ewelina, Viegas, Susana, Caetano, Liliana Aranha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14651
Resumo: EEA Grants Project “EXPOGreen FBR38”.
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spelling Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, PortugalOccupational healthOccupational exposureCemeteriesAspergillusSARS-CoV-2Azole resistanceMycotoxinsCytotoxicityEXPOGreen FBR38IPL/2020/BIOAmbuDrivers_ESTeSLFCT_UIDB/05608/2020FCT_UIDP/05608/2020EEA Grants Project “EXPOGreen FBR38”.Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIOAmbuDrivers_ESTeSL).FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.Cemeteries are potential environmental reservoirs of pathogenic microorganisms from organic matter decomposition. This study aimed to characterize the microbial contamination in three cemeteries, and more specifically in grave diggers’ facilities. One active sampling method (impingement method) and several passive sampling methods (swabs, settled dust, settled dust filters, and electrostatic dust cloths—EDC) were employed. The molecular detection of Aspergillus sections and SARS-CoV-2, as well as mycotoxin analysis, screening of azole resistance, and cytotoxicity measurement were also conducted. Total bacteria contamination was 80 CFU·m−2 in settled dust samples, reached 849 CFU·m−2 in EDC and 20,000 CFU·m−2 in swabs, and ranged from 5000 to 10,000 CFU·m−2 in filters. Gram-negative bacteria (VRBA) were only observed in settled dust samples (2.00 × 105 CFU·m−2). Regarding Aspergillus sp., the highest counts were obtained in DG18 (18.38%) and it was not observed in azole-supplemented SDA media. SARS-CoV-2 and the targeted Aspergillus sections were not detected. Mycophenolic acid was detected in one settled dust sample. Cytotoxic effects were observed for 94.4% filters and 5.6% EDC in A549 lung epithelial cells, and for 50.0% filters and 5.6% EDC in HepG2 cells. Future studies are needed in this occupational setting to implement more focused risk management measures.MDPIRCIPLViegas, CarlaCervantes, RenataDias, MartaGomes, BiancaPena, PedroCarolino, ElisabeteTwarużek, MagdalenaKosicki, RobertSoszczyńska, EwelinaViegas, SusanaCaetano, Liliana Aranha2022-05-23T10:54:22Z2022-052022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14651engViegas C, Cervantes R, Dias M, Gomes B, Pena P, Carolino E, Viegas C, Caetano LA, et al. Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal. Toxins. 2022;14(5):348.10.3390/toxins14050348info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:11:08Zoai:repositorio.ipl.pt:10400.21/14651Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:25.451681Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
title Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
spellingShingle Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
Viegas, Carla
Occupational health
Occupational exposure
Cemeteries
Aspergillus
SARS-CoV-2
Azole resistance
Mycotoxins
Cytotoxicity
EXPOGreen FBR38
IPL/2020/BIOAmbuDrivers_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
title_short Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
title_full Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
title_fullStr Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
title_full_unstemmed Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
title_sort Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal
author Viegas, Carla
author_facet Viegas, Carla
Cervantes, Renata
Dias, Marta
Gomes, Bianca
Pena, Pedro
Carolino, Elisabete
Twarużek, Magdalena
Kosicki, Robert
Soszczyńska, Ewelina
Viegas, Susana
Caetano, Liliana Aranha
author_role author
author2 Cervantes, Renata
Dias, Marta
Gomes, Bianca
Pena, Pedro
Carolino, Elisabete
Twarużek, Magdalena
Kosicki, Robert
Soszczyńska, Ewelina
Viegas, Susana
Caetano, Liliana Aranha
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Cervantes, Renata
Dias, Marta
Gomes, Bianca
Pena, Pedro
Carolino, Elisabete
Twarużek, Magdalena
Kosicki, Robert
Soszczyńska, Ewelina
Viegas, Susana
Caetano, Liliana Aranha
dc.subject.por.fl_str_mv Occupational health
Occupational exposure
Cemeteries
Aspergillus
SARS-CoV-2
Azole resistance
Mycotoxins
Cytotoxicity
EXPOGreen FBR38
IPL/2020/BIOAmbuDrivers_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
topic Occupational health
Occupational exposure
Cemeteries
Aspergillus
SARS-CoV-2
Azole resistance
Mycotoxins
Cytotoxicity
EXPOGreen FBR38
IPL/2020/BIOAmbuDrivers_ESTeSL
FCT_UIDB/05608/2020
FCT_UIDP/05608/2020
description EEA Grants Project “EXPOGreen FBR38”.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-23T10:54:22Z
2022-05
2022-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14651
url http://hdl.handle.net/10400.21/14651
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Cervantes R, Dias M, Gomes B, Pena P, Carolino E, Viegas C, Caetano LA, et al. Six feet under microbiota: microbiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugal. Toxins. 2022;14(5):348.
10.3390/toxins14050348
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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