Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/77696 |
Resumo: | Aquatic hyphomycetes are key microbial decomposers in freshwater that are capable of producing extracellular enzymes targeting complex molecules of leaf litter, thus, being crucial to nutrient cycling in these ecosystems. These fungi are also able to assimilate nutrients (e.g., nitrogen) from stream water, immobilizing these nutrients in the decomposing leaf litter and increasing its nutritional value for higher trophic levels. Evaluating the aquatic hyphomycete functional genetic diversity is, thus, pivotal to understanding the potential impacts of biodiversity loss on nutrient cycling in freshwater. In this work, the inter- and intraspecific taxonomic (ITS1-5.8S-ITS2 region) and functional (nitrate reductase gene) diversity of 40 aquatic hyphomycete strains, belonging to 23 species, was evaluated. A positive correlation was found between the taxonomic and nitrate reductase gene divergences. Interestingly, some cases challenged this trend: <i>Dactylella cylindrospora</i> (Orbiliomycetes) and <i>Thelonectria rubi</i> (Sordariomycetes), which were phylogenetically identical but highly divergent regarding the nitrate reductase gene; and <i>Collembolispora barbata (incertae sedis)</i> and <i>Tetracladium apiense</i> (Leotiomycetes), which exhibited moderate taxonomic divergence but no divergence in the nitrate reductase gene. Additionally, <i>Tricladium chaetocladium</i> (Leotiomycetes) strains were phylogenetically identical but displayed a degree of nitrate reductase gene divergence above the average for the interspecific level. Overall, both inter- and intraspecific functional diversity were observed among aquatic hyphomycetes. |
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Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase geneAquatic hyphomycetesBiodiversityEnzymesFunctional geneFreshwater ecosystemsLeaf litter decompositionScience & TechnologyAquatic hyphomycetes are key microbial decomposers in freshwater that are capable of producing extracellular enzymes targeting complex molecules of leaf litter, thus, being crucial to nutrient cycling in these ecosystems. These fungi are also able to assimilate nutrients (e.g., nitrogen) from stream water, immobilizing these nutrients in the decomposing leaf litter and increasing its nutritional value for higher trophic levels. Evaluating the aquatic hyphomycete functional genetic diversity is, thus, pivotal to understanding the potential impacts of biodiversity loss on nutrient cycling in freshwater. In this work, the inter- and intraspecific taxonomic (ITS1-5.8S-ITS2 region) and functional (nitrate reductase gene) diversity of 40 aquatic hyphomycete strains, belonging to 23 species, was evaluated. A positive correlation was found between the taxonomic and nitrate reductase gene divergences. Interestingly, some cases challenged this trend: <i>Dactylella cylindrospora</i> (Orbiliomycetes) and <i>Thelonectria rubi</i> (Sordariomycetes), which were phylogenetically identical but highly divergent regarding the nitrate reductase gene; and <i>Collembolispora barbata (incertae sedis)</i> and <i>Tetracladium apiense</i> (Leotiomycetes), which exhibited moderate taxonomic divergence but no divergence in the nitrate reductase gene. Additionally, <i>Tricladium chaetocladium</i> (Leotiomycetes) strains were phylogenetically identical but displayed a degree of nitrate reductase gene divergence above the average for the interspecific level. Overall, both inter- and intraspecific functional diversity were observed among aquatic hyphomycetes.This work was supported by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the Portuguese Foundation for Science and Technology (FCT) I.P and by the project STREAMECO-Biodiversity and eco-system functioning under climate change: from the gene to the stream: PTDC/CTA-AMB/31245/2017 funded by FCT.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoMariz, JoanaFranco-Duarte, RicardoCássio, FernandaPascoal, CláudiaFernandes, Isabel Rodrigues2021-12-112021-12-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77696engMariz, J.; Franco-Duarte, R.; Cássio, F.; Pascoal, C.; Fernandes, I. Aquatic Hyphomycete Taxonomic Relatedness Translates into Lower Genetic Divergence of the Nitrate Reductase Gene. J. Fungi 2021, 7, 1066. https://doi.org/10.3390/jof71210662309-608X10.3390/jof71210661066https://www.mdpi.com/2309-608X/7/12/1066info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:42Zoai:repositorium.sdum.uminho.pt:1822/77696Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:25.667930Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
title |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
spellingShingle |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene Mariz, Joana Aquatic hyphomycetes Biodiversity Enzymes Functional gene Freshwater ecosystems Leaf litter decomposition Science & Technology |
title_short |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
title_full |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
title_fullStr |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
title_full_unstemmed |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
title_sort |
Aquatic hyphomycete taxonomic relatedness translates into lower genetic divergence of the nitrate reductase gene |
author |
Mariz, Joana |
author_facet |
Mariz, Joana Franco-Duarte, Ricardo Cássio, Fernanda Pascoal, Cláudia Fernandes, Isabel Rodrigues |
author_role |
author |
author2 |
Franco-Duarte, Ricardo Cássio, Fernanda Pascoal, Cláudia Fernandes, Isabel Rodrigues |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Mariz, Joana Franco-Duarte, Ricardo Cássio, Fernanda Pascoal, Cláudia Fernandes, Isabel Rodrigues |
dc.subject.por.fl_str_mv |
Aquatic hyphomycetes Biodiversity Enzymes Functional gene Freshwater ecosystems Leaf litter decomposition Science & Technology |
topic |
Aquatic hyphomycetes Biodiversity Enzymes Functional gene Freshwater ecosystems Leaf litter decomposition Science & Technology |
description |
Aquatic hyphomycetes are key microbial decomposers in freshwater that are capable of producing extracellular enzymes targeting complex molecules of leaf litter, thus, being crucial to nutrient cycling in these ecosystems. These fungi are also able to assimilate nutrients (e.g., nitrogen) from stream water, immobilizing these nutrients in the decomposing leaf litter and increasing its nutritional value for higher trophic levels. Evaluating the aquatic hyphomycete functional genetic diversity is, thus, pivotal to understanding the potential impacts of biodiversity loss on nutrient cycling in freshwater. In this work, the inter- and intraspecific taxonomic (ITS1-5.8S-ITS2 region) and functional (nitrate reductase gene) diversity of 40 aquatic hyphomycete strains, belonging to 23 species, was evaluated. A positive correlation was found between the taxonomic and nitrate reductase gene divergences. Interestingly, some cases challenged this trend: <i>Dactylella cylindrospora</i> (Orbiliomycetes) and <i>Thelonectria rubi</i> (Sordariomycetes), which were phylogenetically identical but highly divergent regarding the nitrate reductase gene; and <i>Collembolispora barbata (incertae sedis)</i> and <i>Tetracladium apiense</i> (Leotiomycetes), which exhibited moderate taxonomic divergence but no divergence in the nitrate reductase gene. Additionally, <i>Tricladium chaetocladium</i> (Leotiomycetes) strains were phylogenetically identical but displayed a degree of nitrate reductase gene divergence above the average for the interspecific level. Overall, both inter- and intraspecific functional diversity were observed among aquatic hyphomycetes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-11 2021-12-11T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/77696 |
url |
https://hdl.handle.net/1822/77696 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mariz, J.; Franco-Duarte, R.; Cássio, F.; Pascoal, C.; Fernandes, I. Aquatic Hyphomycete Taxonomic Relatedness Translates into Lower Genetic Divergence of the Nitrate Reductase Gene. J. Fungi 2021, 7, 1066. https://doi.org/10.3390/jof7121066 2309-608X 10.3390/jof7121066 1066 https://www.mdpi.com/2309-608X/7/12/1066 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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