Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage

Detalhes bibliográficos
Autor(a) principal: Santos, Sandra P.
Data de Publicação: 2015
Outros Autores: Mitchell, Edward P., Franquelim, Henri G., Castanho, Miguel A. R. B., Abreu, Isabel A., Romão, Célia V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/23164
Resumo: © 2015 FEBS
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spelling Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storageDNAManganeseOligomeric stateOxidative stressReactive oxygen species© 2015 FEBSThe DNA binding proteins from starved cells from Deinococcus radiodurans, Dps1-DR2263 and Dps2-DRB0092, have a common overall structure of hollow spherical dodecamers. Their involvement in the homeostasis of intracellular metal and DNA protection was addressed. Our results show that DrDps proteins are able to oxidize ferrous to ferric iron by oxygen or hydrogen peroxide. The iron stored inside the hollow sphere cavity is fully released. Furthermore, these proteins are able to store and release manganese, suggesting they can play a role in manganese homeostasis as well. The interaction of DrDps with DNA was also addressed. Even though DrDps1 binds both linear and coiled DNA, DrDps2 preferentially binds to coiled DNA, forming different protein–DNA complexes, as clearly shown by atomic force microscopy. DrDps1 (dimer and dodecamer) and DrDps2 can protect DNA against reactive oxygen species, although the protection occurs at different Fe to protein ratios. The difference between DrDps could be the result of the DrDps1 higher iron oxidation rate in the presence of hydrogen peroxide and its higher affinity to bind DNA than in DrDps2. Using cellular extracts obtained from D. radiodurans cultures, we showed that DrDps1 oligomers observed in in vitro conditions are also present in vivo. This indicates that DrDps1 has a structural dynamic plasticity that allows its oligomeric state to change between dimer, trimer and dodecamer. This in turn suggests the existence of a regulation mechanism that modulates the oligomer equilibrium and is dependent on growth stages and environmental conditions.This work was financed by Fundação para a Ciência e Tecnologia through the following Grants: PTDC/BIA-PRO/100365/2008 (C.V.R.), PTDC/QUI-BIQ/100007/2008 (I.A.A.) and PEst-OE/EQB/LA0004/2011. S.P.S. is recipient of PhD Grant PEst-OE/EQB/LA0004/2011. I.A.A. and C.V.R. are recipients of the grants SFRH/BPD/78314/2011 and SFRH/BPD/94050/2013, respectively.WileyRepositório da Universidade de LisboaSantos, Sandra P.Mitchell, Edward P.Franquelim, Henri G.Castanho, Miguel A. R. B.Abreu, Isabel A.Romão, Célia V.2016-03-30T11:22:27Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23164engFEBS Journal 282 (2015) 4307–43271742-464Xdoi:10.1111/febs.13420metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:11:02Zoai:repositorio.ul.pt:10451/23164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:40:37.194304Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
title Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
spellingShingle Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
Santos, Sandra P.
DNA
Manganese
Oligomeric state
Oxidative stress
Reactive oxygen species
title_short Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
title_full Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
title_fullStr Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
title_full_unstemmed Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
title_sort Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
author Santos, Sandra P.
author_facet Santos, Sandra P.
Mitchell, Edward P.
Franquelim, Henri G.
Castanho, Miguel A. R. B.
Abreu, Isabel A.
Romão, Célia V.
author_role author
author2 Mitchell, Edward P.
Franquelim, Henri G.
Castanho, Miguel A. R. B.
Abreu, Isabel A.
Romão, Célia V.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Sandra P.
Mitchell, Edward P.
Franquelim, Henri G.
Castanho, Miguel A. R. B.
Abreu, Isabel A.
Romão, Célia V.
dc.subject.por.fl_str_mv DNA
Manganese
Oligomeric state
Oxidative stress
Reactive oxygen species
topic DNA
Manganese
Oligomeric state
Oxidative stress
Reactive oxygen species
description © 2015 FEBS
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-03-30T11:22:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23164
url http://hdl.handle.net/10451/23164
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FEBS Journal 282 (2015) 4307–4327
1742-464X
doi:10.1111/febs.13420
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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