A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/19625 |
Resumo: | Phlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of <i>P. purpurea,</i> a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in <i>P. purpurea</i> immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of <i>Phlomis purpurea</i> against <i>Phytophthora cinnamomi</i>. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible. |
id |
RCAP_fcfdd41f98045c5df64d49a855bf9ce7 |
---|---|
oai_identifier_str |
oai:sapientia.ualg.pt:10400.1/19625 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomiPhlomis purpureaPhytophthora cinnamomiBiotic stressMetabolomic and transcriptomic integrationPlant immune responsePhlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of <i>P. purpurea,</i> a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in <i>P. purpurea</i> immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of <i>Phlomis purpurea</i> against <i>Phytophthora cinnamomi</i>. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible.2022.07433.CEECINDMDPISapientiaNeves, DinaFigueiredo, AndreiaMaia, MarisaLaczko, EndrePais, Maria SaloméCravador, Alfredo2023-05-26T15:00:26Z2023-05-092023-05-26T13:21:00Z2023-05-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/19625engPlants 12 (10): 1929 (2023)2223-774710.3390/plants12101929info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:32:07Zoai:sapientia.ualg.pt:10400.1/19625Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:09:13.314399Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
title |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
spellingShingle |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi Neves, Dina Phlomis purpurea Phytophthora cinnamomi Biotic stress Metabolomic and transcriptomic integration Plant immune response |
title_short |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
title_full |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
title_fullStr |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
title_full_unstemmed |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
title_sort |
A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomi |
author |
Neves, Dina |
author_facet |
Neves, Dina Figueiredo, Andreia Maia, Marisa Laczko, Endre Pais, Maria Salomé Cravador, Alfredo |
author_role |
author |
author2 |
Figueiredo, Andreia Maia, Marisa Laczko, Endre Pais, Maria Salomé Cravador, Alfredo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Neves, Dina Figueiredo, Andreia Maia, Marisa Laczko, Endre Pais, Maria Salomé Cravador, Alfredo |
dc.subject.por.fl_str_mv |
Phlomis purpurea Phytophthora cinnamomi Biotic stress Metabolomic and transcriptomic integration Plant immune response |
topic |
Phlomis purpurea Phytophthora cinnamomi Biotic stress Metabolomic and transcriptomic integration Plant immune response |
description |
Phlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of <i>P. purpurea,</i> a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in <i>P. purpurea</i> immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of <i>Phlomis purpurea</i> against <i>Phytophthora cinnamomi</i>. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-26T15:00:26Z 2023-05-09 2023-05-26T13:21:00Z 2023-05-09T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/19625 |
url |
http://hdl.handle.net/10400.1/19625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plants 12 (10): 1929 (2023) 2223-7747 10.3390/plants12101929 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133339430420480 |