Characterization of a biofilm formed by Fusarium oxysporum on the human nails
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/75692 |
Resumo: | Background: Fusarium spp. has been considered as an onychomycosis agent, but little is known about the etiopathogenesis of fusarial onychomycosis; thus, the objective of this study was to characterize the fungal-nail interaction and the consequences of the nail infection process by Fusarium oxysporum using the human nail, in an exvivo model. Methods: The kinetic of biofilm production and infection by F. oxysporum using the nail as the only nutritional source were evaluated by scanning electron microscopy, number of culturable cells, metabolic activity, characterization of extracellular matrix, spectroscopy and histopathology analyses. Results: After evaluating the biofilm kinetic over 7 days using different parameters and techniques, it was possible to characterize the Fusarium-nail interaction. Conclusions: This study is a part of a big project aiming to clarify the fusarial pathogenesis and contributes to proving F. oxysporum is able to adapt, grow, develop, and form a biofilm on healthy human nails, which are crucial steps for the invasion process. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Characterization of a biofilm formed by Fusarium oxysporum on the human nailsScience & TechnologyBackground: Fusarium spp. has been considered as an onychomycosis agent, but little is known about the etiopathogenesis of fusarial onychomycosis; thus, the objective of this study was to characterize the fungal-nail interaction and the consequences of the nail infection process by Fusarium oxysporum using the human nail, in an exvivo model. Methods: The kinetic of biofilm production and infection by F. oxysporum using the nail as the only nutritional source were evaluated by scanning electron microscopy, number of culturable cells, metabolic activity, characterization of extracellular matrix, spectroscopy and histopathology analyses. Results: After evaluating the biofilm kinetic over 7 days using different parameters and techniques, it was possible to characterize the Fusarium-nail interaction. Conclusions: This study is a part of a big project aiming to clarify the fusarial pathogenesis and contributes to proving F. oxysporum is able to adapt, grow, develop, and form a biofilm on healthy human nails, which are crucial steps for the invasion process.This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) - Finance Code 001, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Fundacao de Amparo a Pesquisa do Estado do Parana (Fundacao Araucaria) and Financiadora de Estudos e Projetos (FINEP/COMCAP).info:eu-repo/semantics/publishedVersionBlackwell PublishingUniversidade do MinhoVeiga, Flavia Francode Castro-Hoshino, Lidiane VizioliSato, FrancielleBaesso, Mauro LucianoSilva, Sónia CarinaGrassi, M.Svidzinski, Terezinha Inez Estivalet2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/75692engVeiga, Flavia Franco; de Castro-Hoshino, Lidiane Vizioli; Sato, Francielle; Baesso, Mauro Luciano; Silva, Sónia Carina; Grassi, M.; Svidzinski, Terezinha Inez Estivalet, Characterization of a biofilm formed by Fusarium oxysporum on the human nails. International Journal of Dermatology, 61(2), 191-198, 20220011-90591365-463210.1111/ijd.1574734235733https://onlinelibrary.wiley.com/journal/13654632info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:33ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
title |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
spellingShingle |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails Veiga, Flavia Franco Science & Technology |
title_short |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
title_full |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
title_fullStr |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
title_full_unstemmed |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
title_sort |
Characterization of a biofilm formed by Fusarium oxysporum on the human nails |
author |
Veiga, Flavia Franco |
author_facet |
Veiga, Flavia Franco de Castro-Hoshino, Lidiane Vizioli Sato, Francielle Baesso, Mauro Luciano Silva, Sónia Carina Grassi, M. Svidzinski, Terezinha Inez Estivalet |
author_role |
author |
author2 |
de Castro-Hoshino, Lidiane Vizioli Sato, Francielle Baesso, Mauro Luciano Silva, Sónia Carina Grassi, M. Svidzinski, Terezinha Inez Estivalet |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Veiga, Flavia Franco de Castro-Hoshino, Lidiane Vizioli Sato, Francielle Baesso, Mauro Luciano Silva, Sónia Carina Grassi, M. Svidzinski, Terezinha Inez Estivalet |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Background: Fusarium spp. has been considered as an onychomycosis agent, but little is known about the etiopathogenesis of fusarial onychomycosis; thus, the objective of this study was to characterize the fungal-nail interaction and the consequences of the nail infection process by Fusarium oxysporum using the human nail, in an exvivo model. Methods: The kinetic of biofilm production and infection by F. oxysporum using the nail as the only nutritional source were evaluated by scanning electron microscopy, number of culturable cells, metabolic activity, characterization of extracellular matrix, spectroscopy and histopathology analyses. Results: After evaluating the biofilm kinetic over 7 days using different parameters and techniques, it was possible to characterize the Fusarium-nail interaction. Conclusions: This study is a part of a big project aiming to clarify the fusarial pathogenesis and contributes to proving F. oxysporum is able to adapt, grow, develop, and form a biofilm on healthy human nails, which are crucial steps for the invasion process. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02 2022-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/75692 |
url |
https://hdl.handle.net/1822/75692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veiga, Flavia Franco; de Castro-Hoshino, Lidiane Vizioli; Sato, Francielle; Baesso, Mauro Luciano; Silva, Sónia Carina; Grassi, M.; Svidzinski, Terezinha Inez Estivalet, Characterization of a biofilm formed by Fusarium oxysporum on the human nails. International Journal of Dermatology, 61(2), 191-198, 2022 0011-9059 1365-4632 10.1111/ijd.15747 34235733 https://onlinelibrary.wiley.com/journal/13654632 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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repository.mail.fl_str_mv |
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