Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276
Main Author: | |
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Publication Date: | 2020 |
Other Authors: | , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.8/6127 |
Summary: | There has been a growing trend worldwide in the use of squalene and docosahexaenoic acid (DHA), due in part to their recognized health benefits and their range of applications. However, the growth of the industry exploiting these resources has been extremely limited by the scarcity and limitations of raw material, leading to the need to develop more sustainable sources of production. Aurantiochytrium sp. PRA-276, with the ability to produce high concentrations of DHA and squalene, is a promising candidate for the commercial production of these biomolecules. The aim of this study was to determine the cumulative effect of airflow levels and stirring speed of Aurantiochytrium sp. PRA-276 biomass during DHA and squalene production. Different airflow levels (3, 6 and 9 L min-1) and two stirring speeds (150 and 300 rpm) were investigated. The highest biomass production was achieved after 96 hours with 300 rpm stirring speeds and airflow level of 9 L min-1. The maximum total lipids content reached 61.4% of the dried biomass, and the optimal parameters for DHA and squalene production were stirring speed 150 rpm and airflow level 3 L min-1. The maximum yield of DHA production was 4.3 g L-1 at 96 h of fermentation. The highest yield of squalene production was 762.5 mg L-1 at 48 hours of growth. The results show that Aurantiochytrium sp. PRA-276 is a promising resource for microbial DHA and squalene production due to the high yield of DHA and squalene that it generates. Moreover, the capacity for large-scale fermentation of this organism could be useful for the synthesis of biomass related products with high added value. |
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Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276Aurantiochytrium spSqualeneDocosahexaenoic acid (DHA)LipidsHeterotrophic marine algaeThere has been a growing trend worldwide in the use of squalene and docosahexaenoic acid (DHA), due in part to their recognized health benefits and their range of applications. However, the growth of the industry exploiting these resources has been extremely limited by the scarcity and limitations of raw material, leading to the need to develop more sustainable sources of production. Aurantiochytrium sp. PRA-276, with the ability to produce high concentrations of DHA and squalene, is a promising candidate for the commercial production of these biomolecules. The aim of this study was to determine the cumulative effect of airflow levels and stirring speed of Aurantiochytrium sp. PRA-276 biomass during DHA and squalene production. Different airflow levels (3, 6 and 9 L min-1) and two stirring speeds (150 and 300 rpm) were investigated. The highest biomass production was achieved after 96 hours with 300 rpm stirring speeds and airflow level of 9 L min-1. The maximum total lipids content reached 61.4% of the dried biomass, and the optimal parameters for DHA and squalene production were stirring speed 150 rpm and airflow level 3 L min-1. The maximum yield of DHA production was 4.3 g L-1 at 96 h of fermentation. The highest yield of squalene production was 762.5 mg L-1 at 48 hours of growth. The results show that Aurantiochytrium sp. PRA-276 is a promising resource for microbial DHA and squalene production due to the high yield of DHA and squalene that it generates. Moreover, the capacity for large-scale fermentation of this organism could be useful for the synthesis of biomass related products with high added value.GratisIC-OnlinePaulo, M.C.Coutinho, J.Saraiva, J.A.Pinto, C.A.Amaral, R.Melo, M.M.R.Pinheiro, J.Lemos, M.F.L.Cardoso, C.Sapatinha, M.Bandarra, N.M.2021-08-20T14:16:58Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/6127engPaulo, M.C., Coutinho, J., Saraiva, J.A., Pinto, C.A., Amaral, R., Melo, M.M.R., Pinheiro, J., Lemos, M.F.L., Cardoso, C., Sapatinha, M., & Bandarra, N.M. (2020). Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276. Journal of Nutritional Biology, 6(1), 420-429. https://doi.org/10.18314/jnb.v6i1.20602469-414210.18314/jnb.v6i1.2060metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:52:26Zoai:iconline.ipleiria.pt:10400.8/6127Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:26.703717Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
title |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
spellingShingle |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 Paulo, M.C. Aurantiochytrium sp Squalene Docosahexaenoic acid (DHA) Lipids Heterotrophic marine algae |
title_short |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
title_full |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
title_fullStr |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
title_full_unstemmed |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
title_sort |
Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276 |
author |
Paulo, M.C. |
author_facet |
Paulo, M.C. Coutinho, J. Saraiva, J.A. Pinto, C.A. Amaral, R. Melo, M.M.R. Pinheiro, J. Lemos, M.F.L. Cardoso, C. Sapatinha, M. Bandarra, N.M. |
author_role |
author |
author2 |
Coutinho, J. Saraiva, J.A. Pinto, C.A. Amaral, R. Melo, M.M.R. Pinheiro, J. Lemos, M.F.L. Cardoso, C. Sapatinha, M. Bandarra, N.M. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Paulo, M.C. Coutinho, J. Saraiva, J.A. Pinto, C.A. Amaral, R. Melo, M.M.R. Pinheiro, J. Lemos, M.F.L. Cardoso, C. Sapatinha, M. Bandarra, N.M. |
dc.subject.por.fl_str_mv |
Aurantiochytrium sp Squalene Docosahexaenoic acid (DHA) Lipids Heterotrophic marine algae |
topic |
Aurantiochytrium sp Squalene Docosahexaenoic acid (DHA) Lipids Heterotrophic marine algae |
description |
There has been a growing trend worldwide in the use of squalene and docosahexaenoic acid (DHA), due in part to their recognized health benefits and their range of applications. However, the growth of the industry exploiting these resources has been extremely limited by the scarcity and limitations of raw material, leading to the need to develop more sustainable sources of production. Aurantiochytrium sp. PRA-276, with the ability to produce high concentrations of DHA and squalene, is a promising candidate for the commercial production of these biomolecules. The aim of this study was to determine the cumulative effect of airflow levels and stirring speed of Aurantiochytrium sp. PRA-276 biomass during DHA and squalene production. Different airflow levels (3, 6 and 9 L min-1) and two stirring speeds (150 and 300 rpm) were investigated. The highest biomass production was achieved after 96 hours with 300 rpm stirring speeds and airflow level of 9 L min-1. The maximum total lipids content reached 61.4% of the dried biomass, and the optimal parameters for DHA and squalene production were stirring speed 150 rpm and airflow level 3 L min-1. The maximum yield of DHA production was 4.3 g L-1 at 96 h of fermentation. The highest yield of squalene production was 762.5 mg L-1 at 48 hours of growth. The results show that Aurantiochytrium sp. PRA-276 is a promising resource for microbial DHA and squalene production due to the high yield of DHA and squalene that it generates. Moreover, the capacity for large-scale fermentation of this organism could be useful for the synthesis of biomass related products with high added value. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-08-20T14:16:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/6127 |
url |
http://hdl.handle.net/10400.8/6127 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Paulo, M.C., Coutinho, J., Saraiva, J.A., Pinto, C.A., Amaral, R., Melo, M.M.R., Pinheiro, J., Lemos, M.F.L., Cardoso, C., Sapatinha, M., & Bandarra, N.M. (2020). Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276. Journal of Nutritional Biology, 6(1), 420-429. https://doi.org/10.18314/jnb.v6i1.2060 2469-4142 10.18314/jnb.v6i1.2060 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
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openAccess |
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application/pdf |
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Gratis |
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Gratis |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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