Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/30678 |
Resumo: | Introduction: Prostate cancer is one of the most common cancer among men, having a huge impact in their health [1]. This work aimed to evaluate the influence of a decoction extract obtained from C. sativa flowers (CF) on chemically and hormonally induced rat prostate cancer animal model. Material & Methods: All the animal experiments were approved by the Institutional Animals Ethics Committee and by Portuguese national authorities (DGAV no 021326). Forty male Wistar Unilever rats were randomly divided into four groups: control group (n=10), induced group (n=15), CF control group (n=5) and CF induced group (n=10). Animals from induced groups received a multistep induction protocol, which consisted of sequential administration of flutamide, testosterone propionate, the carcinogenic agent MNU and crystalline testosterone. The CF extract, rich in ellagitannins especially trigalloy-HHDP- glucose, was administered in the drinking water (3 mg/animal/day) for 49 weeks. Animals were sacrificed at 61 weeks of age and organs were collected, weighed and processed for light microscopy. Data were analysed using SPSS and GraphPad Prism software. Results: There were no significant differences in relative mean liver weight among groups exposed and not exposed to the CF extract and no animals developed severe hepatic changes. Animals from CF induced group developed less prostatic intraepithelial neoplasia than induced group. Also, animals exposed to the CF extract did not present areas of inflammation of the dorsolateral prostate lobe greater than 50% unlike the groups not exposed (p<0.05). The administration of CF in induced animals was able to decrease the activity of CAT and GST by 36% and 20%, respectively (p<0.05). Conclusions: These results suggest that CF extract was well tolerate by the animals and did not cause severe hepatic and renal toxicity. C. sativa flowers extract may be used as chemopreventive agent against prostate cancer and seems to have an antioxidant role. |
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Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer modelIntroduction: Prostate cancer is one of the most common cancer among men, having a huge impact in their health [1]. This work aimed to evaluate the influence of a decoction extract obtained from C. sativa flowers (CF) on chemically and hormonally induced rat prostate cancer animal model. Material & Methods: All the animal experiments were approved by the Institutional Animals Ethics Committee and by Portuguese national authorities (DGAV no 021326). Forty male Wistar Unilever rats were randomly divided into four groups: control group (n=10), induced group (n=15), CF control group (n=5) and CF induced group (n=10). Animals from induced groups received a multistep induction protocol, which consisted of sequential administration of flutamide, testosterone propionate, the carcinogenic agent MNU and crystalline testosterone. The CF extract, rich in ellagitannins especially trigalloy-HHDP- glucose, was administered in the drinking water (3 mg/animal/day) for 49 weeks. Animals were sacrificed at 61 weeks of age and organs were collected, weighed and processed for light microscopy. Data were analysed using SPSS and GraphPad Prism software. Results: There were no significant differences in relative mean liver weight among groups exposed and not exposed to the CF extract and no animals developed severe hepatic changes. Animals from CF induced group developed less prostatic intraepithelial neoplasia than induced group. Also, animals exposed to the CF extract did not present areas of inflammation of the dorsolateral prostate lobe greater than 50% unlike the groups not exposed (p<0.05). The administration of CF in induced animals was able to decrease the activity of CAT and GST by 36% and 20%, respectively (p<0.05). Conclusions: These results suggest that CF extract was well tolerate by the animals and did not cause severe hepatic and renal toxicity. C. sativa flowers extract may be used as chemopreventive agent against prostate cancer and seems to have an antioxidant role.L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia2022-01-07T22:48:48Z2022-01-072020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/30678http://hdl.handle.net/10174/30678engNascimento-Gonçalves E, Silva M, Seixas F, Fardilha M, Ferreira R, Neuparth MJ, Faustino-Rocha AI, Colaço B, Venâncio C, Rosa E, Barros L, Ferreira ICFR, Oliveira MM, Peixoto F, Oliveira PA. 2020. Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model. L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia, 5 a 7 de fevereiro, Coimbra, Portugal.simnaonaondndndndndndanafaustino@uevora.ptndndndndndndndnd206Nascimento-Gonçalves, ElisabeteSilva, MSeixas, FernandaFardilha, MargaridaFerreira, RitaNeuparth, Maria JoãoFaustino-Rocha, Ana IsabelColaço, BrunoVenâncio, CarlosRosa, EduardoBarros, LFerreira, Isabel CFROliveira, MMPeixoto, FranciscoOliveira, Paula Alexandrainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:27:39Zoai:dspace.uevora.pt:10174/30678Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:19:35.079023Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
title |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
spellingShingle |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model Nascimento-Gonçalves, Elisabete |
title_short |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
title_full |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
title_fullStr |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
title_full_unstemmed |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
title_sort |
Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model |
author |
Nascimento-Gonçalves, Elisabete |
author_facet |
Nascimento-Gonçalves, Elisabete Silva, M Seixas, Fernanda Fardilha, Margarida Ferreira, Rita Neuparth, Maria João Faustino-Rocha, Ana Isabel Colaço, Bruno Venâncio, Carlos Rosa, Eduardo Barros, L Ferreira, Isabel CFR Oliveira, MM Peixoto, Francisco Oliveira, Paula Alexandra |
author_role |
author |
author2 |
Silva, M Seixas, Fernanda Fardilha, Margarida Ferreira, Rita Neuparth, Maria João Faustino-Rocha, Ana Isabel Colaço, Bruno Venâncio, Carlos Rosa, Eduardo Barros, L Ferreira, Isabel CFR Oliveira, MM Peixoto, Francisco Oliveira, Paula Alexandra |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento-Gonçalves, Elisabete Silva, M Seixas, Fernanda Fardilha, Margarida Ferreira, Rita Neuparth, Maria João Faustino-Rocha, Ana Isabel Colaço, Bruno Venâncio, Carlos Rosa, Eduardo Barros, L Ferreira, Isabel CFR Oliveira, MM Peixoto, Francisco Oliveira, Paula Alexandra |
description |
Introduction: Prostate cancer is one of the most common cancer among men, having a huge impact in their health [1]. This work aimed to evaluate the influence of a decoction extract obtained from C. sativa flowers (CF) on chemically and hormonally induced rat prostate cancer animal model. Material & Methods: All the animal experiments were approved by the Institutional Animals Ethics Committee and by Portuguese national authorities (DGAV no 021326). Forty male Wistar Unilever rats were randomly divided into four groups: control group (n=10), induced group (n=15), CF control group (n=5) and CF induced group (n=10). Animals from induced groups received a multistep induction protocol, which consisted of sequential administration of flutamide, testosterone propionate, the carcinogenic agent MNU and crystalline testosterone. The CF extract, rich in ellagitannins especially trigalloy-HHDP- glucose, was administered in the drinking water (3 mg/animal/day) for 49 weeks. Animals were sacrificed at 61 weeks of age and organs were collected, weighed and processed for light microscopy. Data were analysed using SPSS and GraphPad Prism software. Results: There were no significant differences in relative mean liver weight among groups exposed and not exposed to the CF extract and no animals developed severe hepatic changes. Animals from CF induced group developed less prostatic intraepithelial neoplasia than induced group. Also, animals exposed to the CF extract did not present areas of inflammation of the dorsolateral prostate lobe greater than 50% unlike the groups not exposed (p<0.05). The administration of CF in induced animals was able to decrease the activity of CAT and GST by 36% and 20%, respectively (p<0.05). Conclusions: These results suggest that CF extract was well tolerate by the animals and did not cause severe hepatic and renal toxicity. C. sativa flowers extract may be used as chemopreventive agent against prostate cancer and seems to have an antioxidant role. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01T00:00:00Z 2022-01-07T22:48:48Z 2022-01-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/30678 http://hdl.handle.net/10174/30678 |
url |
http://hdl.handle.net/10174/30678 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nascimento-Gonçalves E, Silva M, Seixas F, Fardilha M, Ferreira R, Neuparth MJ, Faustino-Rocha AI, Colaço B, Venâncio C, Rosa E, Barros L, Ferreira ICFR, Oliveira MM, Peixoto F, Oliveira PA. 2020. Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model. L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia, 5 a 7 de fevereiro, Coimbra, Portugal. sim nao nao nd nd nd nd nd nd anafaustino@uevora.pt nd nd nd nd nd nd nd nd 206 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia |
publisher.none.fl_str_mv |
L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136677117034496 |