Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis

Detalhes bibliográficos
Autor(a) principal: Araújo, Cecília Alves
Data de Publicação: 2004
Outros Autores: Almeida, M. J., Sousa, Maria João, Leão, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/2266
Resumo: The freeze-stress responses to prolonged storage at - 20 °C in Torulaspora delbrueckii PYCC5323 were investigated. In this yeast no loss of cell viability was observed for at least 120 days during freezing at - 20ºC, whereas a loss of 80% was observed in a commercial baker's yeast after 15 days. In the former strain, freeze resistance was dependent on an adaptation process. The primary cell target of freeze stress was the plasma membrane, preservation of it’s integrity being related with a lower increase of lipid peroxidation and with higher resistance to H2O2, but not with intracellular trehalose concentration.
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spelling Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basisTorulaspora delbrueckiiFreeze-toleranceMembrane-integrityBaker’s yeastlipid peroxidationScience & TechnologyThe freeze-stress responses to prolonged storage at - 20 °C in Torulaspora delbrueckii PYCC5323 were investigated. In this yeast no loss of cell viability was observed for at least 120 days during freezing at - 20ºC, whereas a loss of 80% was observed in a commercial baker's yeast after 15 days. In the former strain, freeze resistance was dependent on an adaptation process. The primary cell target of freeze stress was the plasma membrane, preservation of it’s integrity being related with a lower increase of lipid peroxidation and with higher resistance to H2O2, but not with intracellular trehalose concentration.Fundação para a Ciência e a Tecnologia (FCT) - grant (PRAXIS/XXI/BD/21543/99).Oxford University PressUniversidade do MinhoAraújo, Cecília AlvesAlmeida, M. J.Sousa, Maria JoãoLeão, Cecília20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttps://hdl.handle.net/1822/2266engAlves-Araújo, C., Almeida, M. J., Sousa, M. J., & Leão, C. (2004, November). Freeze tolerance of the yeastTorulaspora delbrueckii: cellular and biochemical basis. FEMS Microbiology Letters. Oxford University Press (OUP). http://doi.org/10.1016/j.femsle.2004.09.0080378-109710.1016/j.femsle.2004.09.00815500973http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#descriptioninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:37Zoai:repositorium.sdum.uminho.pt:1822/2266Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:47.986591Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
title Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
spellingShingle Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
Araújo, Cecília Alves
Torulaspora delbrueckii
Freeze-tolerance
Membrane-integrity
Baker’s yeast
lipid peroxidation
Science & Technology
title_short Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
title_full Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
title_fullStr Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
title_full_unstemmed Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
title_sort Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis
author Araújo, Cecília Alves
author_facet Araújo, Cecília Alves
Almeida, M. J.
Sousa, Maria João
Leão, Cecília
author_role author
author2 Almeida, M. J.
Sousa, Maria João
Leão, Cecília
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Araújo, Cecília Alves
Almeida, M. J.
Sousa, Maria João
Leão, Cecília
dc.subject.por.fl_str_mv Torulaspora delbrueckii
Freeze-tolerance
Membrane-integrity
Baker’s yeast
lipid peroxidation
Science & Technology
topic Torulaspora delbrueckii
Freeze-tolerance
Membrane-integrity
Baker’s yeast
lipid peroxidation
Science & Technology
description The freeze-stress responses to prolonged storage at - 20 °C in Torulaspora delbrueckii PYCC5323 were investigated. In this yeast no loss of cell viability was observed for at least 120 days during freezing at - 20ºC, whereas a loss of 80% was observed in a commercial baker's yeast after 15 days. In the former strain, freeze resistance was dependent on an adaptation process. The primary cell target of freeze stress was the plasma membrane, preservation of it’s integrity being related with a lower increase of lipid peroxidation and with higher resistance to H2O2, but not with intracellular trehalose concentration.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/2266
url https://hdl.handle.net/1822/2266
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves-Araújo, C., Almeida, M. J., Sousa, M. J., & Leão, C. (2004, November). Freeze tolerance of the yeastTorulaspora delbrueckii: cellular and biochemical basis. FEMS Microbiology Letters. Oxford University Press (OUP). http://doi.org/10.1016/j.femsle.2004.09.008
0378-1097
10.1016/j.femsle.2004.09.008
15500973
http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#description
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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