Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Matéria (Rio de Janeiro. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762008000100002 |
Resumo: | Poly(3-hydroxybutyrate) was produced in fed-batch cultures of Ralstonia eutropha DSM 428 and Alcaligenes latus ATCC 29712 on a mineral medium with different carbon sources such as sucrose, sodium lactate, lactic acid, soybean oil and fatty acid. The bacteria converted the different carbon sources supplied into P3HB. The best results were obtained when lactate or soybean oil were supplied as the sole carbon source. The range of number average molar mass (Mn) for the polymers, analyzed by Gel Permeation Chromatography was 1.65 to 0.79 x 10(5) g mol-1. FTIR spectroscopy revealed a characteristic absorbance associated with polyester structures. The crystallinity degree, determinate from X-ray diffractograms, was about 69% in all synthesized polymers. The thermal properties associated to semicrystalline polymers indicated a glass transition at 0.1ºC and a melting point at about 175ºC and enthalpy of 63- 89 J g-1. The ¹H-NMR and 13C-NMR spectra of the polymers were in agreement with the calculated chemical shifts associated with P3HB structures. |
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Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sourcespoly(3-hydroxybutyrate)biodegradable polymercharacterization and renewable sourcePoly(3-hydroxybutyrate) was produced in fed-batch cultures of Ralstonia eutropha DSM 428 and Alcaligenes latus ATCC 29712 on a mineral medium with different carbon sources such as sucrose, sodium lactate, lactic acid, soybean oil and fatty acid. The bacteria converted the different carbon sources supplied into P3HB. The best results were obtained when lactate or soybean oil were supplied as the sole carbon source. The range of number average molar mass (Mn) for the polymers, analyzed by Gel Permeation Chromatography was 1.65 to 0.79 x 10(5) g mol-1. FTIR spectroscopy revealed a characteristic absorbance associated with polyester structures. The crystallinity degree, determinate from X-ray diffractograms, was about 69% in all synthesized polymers. The thermal properties associated to semicrystalline polymers indicated a glass transition at 0.1ºC and a melting point at about 175ºC and enthalpy of 63- 89 J g-1. The ¹H-NMR and 13C-NMR spectra of the polymers were in agreement with the calculated chemical shifts associated with P3HB structures.Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiroem cooperação com a Associação Brasileira do Hidrogênio, ABH22008-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762008000100002Matéria (Rio de Janeiro) v.13 n.1 2008reponame:Matéria (Rio de Janeiro. Online)instname:Matéria (Rio de Janeiro. Online)instacron:RLAM10.1590/S1517-70762008000100002info:eu-repo/semantics/openAccessPereira,S.M.F.Rodriguez,R.S.Gomes,J.G.C.eng2008-05-12T00:00:00Zoai:scielo:S1517-70762008000100002Revistahttp://www.materia.coppe.ufrj.br/https://old.scielo.br/oai/scielo-oai.php||materia@labh2.coppe.ufrj.br1517-70761517-7076opendoar:2008-05-12T00:00Matéria (Rio de Janeiro. Online) - Matéria (Rio de Janeiro. Online)false |
dc.title.none.fl_str_mv |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
title |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
spellingShingle |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources Pereira,S.M.F. poly(3-hydroxybutyrate) biodegradable polymer characterization and renewable source |
title_short |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
title_full |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
title_fullStr |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
title_full_unstemmed |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
title_sort |
Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources |
author |
Pereira,S.M.F. |
author_facet |
Pereira,S.M.F. Rodriguez,R.S. Gomes,J.G.C. |
author_role |
author |
author2 |
Rodriguez,R.S. Gomes,J.G.C. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Pereira,S.M.F. Rodriguez,R.S. Gomes,J.G.C. |
dc.subject.por.fl_str_mv |
poly(3-hydroxybutyrate) biodegradable polymer characterization and renewable source |
topic |
poly(3-hydroxybutyrate) biodegradable polymer characterization and renewable source |
description |
Poly(3-hydroxybutyrate) was produced in fed-batch cultures of Ralstonia eutropha DSM 428 and Alcaligenes latus ATCC 29712 on a mineral medium with different carbon sources such as sucrose, sodium lactate, lactic acid, soybean oil and fatty acid. The bacteria converted the different carbon sources supplied into P3HB. The best results were obtained when lactate or soybean oil were supplied as the sole carbon source. The range of number average molar mass (Mn) for the polymers, analyzed by Gel Permeation Chromatography was 1.65 to 0.79 x 10(5) g mol-1. FTIR spectroscopy revealed a characteristic absorbance associated with polyester structures. The crystallinity degree, determinate from X-ray diffractograms, was about 69% in all synthesized polymers. The thermal properties associated to semicrystalline polymers indicated a glass transition at 0.1ºC and a melting point at about 175ºC and enthalpy of 63- 89 J g-1. The ¹H-NMR and 13C-NMR spectra of the polymers were in agreement with the calculated chemical shifts associated with P3HB structures. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762008000100002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762008000100002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-70762008000100002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiro em cooperação com a Associação Brasileira do Hidrogênio, ABH2 |
publisher.none.fl_str_mv |
Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiro em cooperação com a Associação Brasileira do Hidrogênio, ABH2 |
dc.source.none.fl_str_mv |
Matéria (Rio de Janeiro) v.13 n.1 2008 reponame:Matéria (Rio de Janeiro. Online) instname:Matéria (Rio de Janeiro. Online) instacron:RLAM |
instname_str |
Matéria (Rio de Janeiro. Online) |
instacron_str |
RLAM |
institution |
RLAM |
reponame_str |
Matéria (Rio de Janeiro. Online) |
collection |
Matéria (Rio de Janeiro. Online) |
repository.name.fl_str_mv |
Matéria (Rio de Janeiro. Online) - Matéria (Rio de Janeiro. Online) |
repository.mail.fl_str_mv |
||materia@labh2.coppe.ufrj.br |
_version_ |
1752126687087165440 |