DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae)
Main Author: | |
---|---|
Publication Date: | 2018 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Acta Botanica Brasilica |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511 |
Summary: | ABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoamerica were applied to 16 species, together with 63 morphological characters, for species characterization. Molecular and morphological data were analysed by cluster analysis, followed by a cluster sharpness test and the construction of a heat map to visualize the association of characters with species groups. A multivariate regression tree determined the number of loci needed for species identification. Forty-five primers were transferable to at least four species. Molecular data were more efficient in detecting sharp groups than morphological data. Species groups delimited by a set of shared morphological characters were differentiated based on molecular data. The sixteen studied species could be separated by nine primers, demonstrating the cross-species transferability of SSR markers and the usefulness of DNA fingerprinting for both the delimitation and the identification of species of Piper. |
id |
SBB-1_b8f87b812eafc6f5a3c63fa30fbb2e4a |
---|---|
oai_identifier_str |
oai:scielo:S0102-33062018000400511 |
network_acronym_str |
SBB-1 |
network_name_str |
Acta Botanica Brasilica |
repository_id_str |
|
spelling |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae)heat mapmicrosatellitesmorphologymultivariate analysestaxonomyABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoamerica were applied to 16 species, together with 63 morphological characters, for species characterization. Molecular and morphological data were analysed by cluster analysis, followed by a cluster sharpness test and the construction of a heat map to visualize the association of characters with species groups. A multivariate regression tree determined the number of loci needed for species identification. Forty-five primers were transferable to at least four species. Molecular data were more efficient in detecting sharp groups than morphological data. Species groups delimited by a set of shared morphological characters were differentiated based on molecular data. The sixteen studied species could be separated by nine primers, demonstrating the cross-species transferability of SSR markers and the usefulness of DNA fingerprinting for both the delimitation and the identification of species of Piper.Sociedade Botânica do Brasil2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511Acta Botanica Brasilica v.32 n.4 2018reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/0102-33062017abb0268info:eu-repo/semantics/openAccessChrist,Jheniffer AbeldtHollunder,Renan KöppCarvalho,Marina SantosFerreira,Marcia Flores da SilvaGarbin,Mário LuísCarrijo,Tatiana Tavareseng2018-09-05T00:00:00Zoai:scielo:S0102-33062018000400511Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2018-09-05T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false |
dc.title.none.fl_str_mv |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
title |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
spellingShingle |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) Christ,Jheniffer Abeldt heat map microsatellites morphology multivariate analyses taxonomy |
title_short |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
title_full |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
title_fullStr |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
title_full_unstemmed |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
title_sort |
DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) |
author |
Christ,Jheniffer Abeldt |
author_facet |
Christ,Jheniffer Abeldt Hollunder,Renan Köpp Carvalho,Marina Santos Ferreira,Marcia Flores da Silva Garbin,Mário Luís Carrijo,Tatiana Tavares |
author_role |
author |
author2 |
Hollunder,Renan Köpp Carvalho,Marina Santos Ferreira,Marcia Flores da Silva Garbin,Mário Luís Carrijo,Tatiana Tavares |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Christ,Jheniffer Abeldt Hollunder,Renan Köpp Carvalho,Marina Santos Ferreira,Marcia Flores da Silva Garbin,Mário Luís Carrijo,Tatiana Tavares |
dc.subject.por.fl_str_mv |
heat map microsatellites morphology multivariate analyses taxonomy |
topic |
heat map microsatellites morphology multivariate analyses taxonomy |
description |
ABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoamerica were applied to 16 species, together with 63 morphological characters, for species characterization. Molecular and morphological data were analysed by cluster analysis, followed by a cluster sharpness test and the construction of a heat map to visualize the association of characters with species groups. A multivariate regression tree determined the number of loci needed for species identification. Forty-five primers were transferable to at least four species. Molecular data were more efficient in detecting sharp groups than morphological data. Species groups delimited by a set of shared morphological characters were differentiated based on molecular data. The sixteen studied species could be separated by nine primers, demonstrating the cross-species transferability of SSR markers and the usefulness of DNA fingerprinting for both the delimitation and the identification of species of Piper. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0102-33062017abb0268 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
dc.source.none.fl_str_mv |
Acta Botanica Brasilica v.32 n.4 2018 reponame:Acta Botanica Brasilica instname:Sociedade Botânica do Brasil (SBB) instacron:SBB |
instname_str |
Sociedade Botânica do Brasil (SBB) |
instacron_str |
SBB |
institution |
SBB |
reponame_str |
Acta Botanica Brasilica |
collection |
Acta Botanica Brasilica |
repository.name.fl_str_mv |
Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB) |
repository.mail.fl_str_mv |
acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com |
_version_ |
1752126662962577408 |