Comparison of two surgical techniques for creating an acute myocardial infarct in rats

Detalhes bibliográficos
Autor(a) principal: Capriglione,Luiz Guilherme Achcar
Data de Publicação: 2014
Outros Autores: Barchiki,Fabiane, Ottoboni,Gabriel Sales, Miyague,Nelson Itiro, Suss,Paula Hansen, Rebelatto,Carmen Lúcia Kuniyoshi, Pimpão,Cláudia Turra, Senegaglia,Alexandra Cristina, Brofman,Paulo Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Cardiovascular Surgery (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000400006
Resumo: Objective: To perform a comparative assessment of two surgical techniques that are used creating an acute myocardial infarc by occluding the left anterior descending coronary artery in order to generate rats with a left ventricular ejection fraction of less than 40%. Methods: The study was completely randomized and comprised 89 halothane-anaesthetised rats, which were divided into three groups. The control group (SHAM) comprised fourteen rats, whose left anterior descending coronary artery was not occluded. Group 1 (G1): comprised by 35 endotracheally intubated and mechanically ventilated rats, whose left anterior descending coronary artery was occluded. Group 2 (G2): comprised 40 rats being manually ventilated using a nasal respirator whose left anterior descending coronary artery was occluded. Other differences between the two techniques include the method of performing the thoracotomy and removing the pericardium in order to expose the heart, and the use of different methods and suture types for closing the thorax. Seven days after surgery, the cardiac function of all surviving rats was determined by echocardiography. Results: No rats SHAM group had progressed to death or had left ventricular ejection fraction less than 40%. Nine of the 16 surviving G1 rats (56.3%) and six of the 20 surviving G2 rats (30%) had a left ventricular ejection fraction of less than 40%. Conclusion: The results indicate a tendency of the technique used in G1 to be better than in G2. This improvement is probably due to the greater duration of the open thorax, which reduces the pressure over time from the surgeon, allowing occlusion of left anterior descending coronary artery with higher accuracy.
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spelling Comparison of two surgical techniques for creating an acute myocardial infarct in ratsMyocardial infarctionEchocardiographyModels, Cardiovascular Objective: To perform a comparative assessment of two surgical techniques that are used creating an acute myocardial infarc by occluding the left anterior descending coronary artery in order to generate rats with a left ventricular ejection fraction of less than 40%. Methods: The study was completely randomized and comprised 89 halothane-anaesthetised rats, which were divided into three groups. The control group (SHAM) comprised fourteen rats, whose left anterior descending coronary artery was not occluded. Group 1 (G1): comprised by 35 endotracheally intubated and mechanically ventilated rats, whose left anterior descending coronary artery was occluded. Group 2 (G2): comprised 40 rats being manually ventilated using a nasal respirator whose left anterior descending coronary artery was occluded. Other differences between the two techniques include the method of performing the thoracotomy and removing the pericardium in order to expose the heart, and the use of different methods and suture types for closing the thorax. Seven days after surgery, the cardiac function of all surviving rats was determined by echocardiography. Results: No rats SHAM group had progressed to death or had left ventricular ejection fraction less than 40%. Nine of the 16 surviving G1 rats (56.3%) and six of the 20 surviving G2 rats (30%) had a left ventricular ejection fraction of less than 40%. Conclusion: The results indicate a tendency of the technique used in G1 to be better than in G2. This improvement is probably due to the greater duration of the open thorax, which reduces the pressure over time from the surgeon, allowing occlusion of left anterior descending coronary artery with higher accuracy. Sociedade Brasileira de Cirurgia Cardiovascular2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000400006Brazilian Journal of Cardiovascular Surgery v.29 n.4 2014reponame:Brazilian Journal of Cardiovascular Surgery (Online)instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)instacron:SBCCV10.5935/1678-9741.20140075info:eu-repo/semantics/openAccessCapriglione,Luiz Guilherme AchcarBarchiki,FabianeOttoboni,Gabriel SalesMiyague,Nelson ItiroSuss,Paula HansenRebelatto,Carmen Lúcia KuniyoshiPimpão,Cláudia TurraSenegaglia,Alexandra CristinaBrofman,Paulo Robertoeng2015-04-09T00:00:00Zoai:scielo:S0102-76382014000400006Revistahttp://www.rbccv.org.br/https://old.scielo.br/oai/scielo-oai.php||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br1678-97410102-7638opendoar:2015-04-09T00:00Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)false
dc.title.none.fl_str_mv Comparison of two surgical techniques for creating an acute myocardial infarct in rats
title Comparison of two surgical techniques for creating an acute myocardial infarct in rats
spellingShingle Comparison of two surgical techniques for creating an acute myocardial infarct in rats
Capriglione,Luiz Guilherme Achcar
Myocardial infarction
Echocardiography
Models, Cardiovascular
title_short Comparison of two surgical techniques for creating an acute myocardial infarct in rats
title_full Comparison of two surgical techniques for creating an acute myocardial infarct in rats
title_fullStr Comparison of two surgical techniques for creating an acute myocardial infarct in rats
title_full_unstemmed Comparison of two surgical techniques for creating an acute myocardial infarct in rats
title_sort Comparison of two surgical techniques for creating an acute myocardial infarct in rats
author Capriglione,Luiz Guilherme Achcar
author_facet Capriglione,Luiz Guilherme Achcar
Barchiki,Fabiane
Ottoboni,Gabriel Sales
Miyague,Nelson Itiro
Suss,Paula Hansen
Rebelatto,Carmen Lúcia Kuniyoshi
Pimpão,Cláudia Turra
Senegaglia,Alexandra Cristina
Brofman,Paulo Roberto
author_role author
author2 Barchiki,Fabiane
Ottoboni,Gabriel Sales
Miyague,Nelson Itiro
Suss,Paula Hansen
Rebelatto,Carmen Lúcia Kuniyoshi
Pimpão,Cláudia Turra
Senegaglia,Alexandra Cristina
Brofman,Paulo Roberto
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Capriglione,Luiz Guilherme Achcar
Barchiki,Fabiane
Ottoboni,Gabriel Sales
Miyague,Nelson Itiro
Suss,Paula Hansen
Rebelatto,Carmen Lúcia Kuniyoshi
Pimpão,Cláudia Turra
Senegaglia,Alexandra Cristina
Brofman,Paulo Roberto
dc.subject.por.fl_str_mv Myocardial infarction
Echocardiography
Models, Cardiovascular
topic Myocardial infarction
Echocardiography
Models, Cardiovascular
description Objective: To perform a comparative assessment of two surgical techniques that are used creating an acute myocardial infarc by occluding the left anterior descending coronary artery in order to generate rats with a left ventricular ejection fraction of less than 40%. Methods: The study was completely randomized and comprised 89 halothane-anaesthetised rats, which were divided into three groups. The control group (SHAM) comprised fourteen rats, whose left anterior descending coronary artery was not occluded. Group 1 (G1): comprised by 35 endotracheally intubated and mechanically ventilated rats, whose left anterior descending coronary artery was occluded. Group 2 (G2): comprised 40 rats being manually ventilated using a nasal respirator whose left anterior descending coronary artery was occluded. Other differences between the two techniques include the method of performing the thoracotomy and removing the pericardium in order to expose the heart, and the use of different methods and suture types for closing the thorax. Seven days after surgery, the cardiac function of all surviving rats was determined by echocardiography. Results: No rats SHAM group had progressed to death or had left ventricular ejection fraction less than 40%. Nine of the 16 surviving G1 rats (56.3%) and six of the 20 surviving G2 rats (30%) had a left ventricular ejection fraction of less than 40%. Conclusion: The results indicate a tendency of the technique used in G1 to be better than in G2. This improvement is probably due to the greater duration of the open thorax, which reduces the pressure over time from the surgeon, allowing occlusion of left anterior descending coronary artery with higher accuracy.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000400006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000400006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/1678-9741.20140075
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Cirurgia Cardiovascular
publisher.none.fl_str_mv Sociedade Brasileira de Cirurgia Cardiovascular
dc.source.none.fl_str_mv Brazilian Journal of Cardiovascular Surgery v.29 n.4 2014
reponame:Brazilian Journal of Cardiovascular Surgery (Online)
instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)
instacron:SBCCV
instname_str Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)
instacron_str SBCCV
institution SBCCV
reponame_str Brazilian Journal of Cardiovascular Surgery (Online)
collection Brazilian Journal of Cardiovascular Surgery (Online)
repository.name.fl_str_mv Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)
repository.mail.fl_str_mv ||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br
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