Cytotoxic and Genotoxic Effects of Macroptilium lathyroides
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Planta daninha (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244 |
Resumo: | ABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities. |
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Cytotoxic and Genotoxic Effects of Macroptilium lathyroidesabnormalitiesbioassayscell divisionphasey beanmitotic indexABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.Sociedade Brasileira da Ciência das Plantas Daninhas 2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244Planta Daninha v.36 2018reponame:Planta daninha (Online)instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)instacron:SBCPD10.1590/s0100-83582018360100043info:eu-repo/semantics/openAccessSILVA,M.S.A.YAMASHITA,O.M.ROSSI,A.A.B.CONCENÇO,G.CARVALHO,M.A.C.FELITO,R.A.eng2018-07-11T00:00:00Zoai:scielo:S0100-83582018000100244Revistahttp://revistas.cpd.ufv.br/pdaninhaweb/https://old.scielo.br/oai/scielo-oai.php||rpdaninha@gmail.com1806-96810100-8358opendoar:2018-07-11T00:00Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)false |
dc.title.none.fl_str_mv |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
title |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
spellingShingle |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides SILVA,M.S.A. abnormalities bioassays cell division phasey bean mitotic index |
title_short |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
title_full |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
title_fullStr |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
title_full_unstemmed |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
title_sort |
Cytotoxic and Genotoxic Effects of Macroptilium lathyroides |
author |
SILVA,M.S.A. |
author_facet |
SILVA,M.S.A. YAMASHITA,O.M. ROSSI,A.A.B. CONCENÇO,G. CARVALHO,M.A.C. FELITO,R.A. |
author_role |
author |
author2 |
YAMASHITA,O.M. ROSSI,A.A.B. CONCENÇO,G. CARVALHO,M.A.C. FELITO,R.A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
SILVA,M.S.A. YAMASHITA,O.M. ROSSI,A.A.B. CONCENÇO,G. CARVALHO,M.A.C. FELITO,R.A. |
dc.subject.por.fl_str_mv |
abnormalities bioassays cell division phasey bean mitotic index |
topic |
abnormalities bioassays cell division phasey bean mitotic index |
description |
ABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0100-83582018360100043 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
dc.source.none.fl_str_mv |
Planta Daninha v.36 2018 reponame:Planta daninha (Online) instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) instacron:SBCPD |
instname_str |
Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
instacron_str |
SBCPD |
institution |
SBCPD |
reponame_str |
Planta daninha (Online) |
collection |
Planta daninha (Online) |
repository.name.fl_str_mv |
Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
repository.mail.fl_str_mv |
||rpdaninha@gmail.com |
_version_ |
1752126495731482624 |