Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Ciência do Solo (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400009 |
Resumo: | Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated from Epidendrum denticulatum, and a phytopathogenic fungus, Ceratorhiza sp. AGC, were grouped based on the profile of their fatty acids, which was assessed by the Euclidian and Mahalanobis distances and the UPGMA method. Dendrograms distinguished the phytopathogenical isolate of Ceratorhiza sp. AGC from the mycorrhizal fungi studied. The symbionts of E. secundum were grouped into two clades, one containing Epulorhiza sp.1 isolates and the other the Epulorhiza sp.2 isolate. The similarity between the symbionts of E. denticulatum and Epulorhiza spp. fungi suggests that symbionts found in E. denticulatum may be identified as Epulorhiza. These results were corroborated by the analysis of the rDNA ITS region. The dendrogram constructed based on the Mahalanobis distance differentiated the clades most clearly. Fatty acid composition analysis proved to be a useful tool for characterizing and identifying Rhizoctonia-like mycorrhizal fungi. |
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Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp.Rhizoctonia-like fungiEpidendrum spp. orchidsFAMEMIDI methodITS sequence analysisfungos rizoctonioidesorquídeas Epidendrum sppFAMEmétodo MIDIanálise da sequência da região ITSRhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated from Epidendrum denticulatum, and a phytopathogenic fungus, Ceratorhiza sp. AGC, were grouped based on the profile of their fatty acids, which was assessed by the Euclidian and Mahalanobis distances and the UPGMA method. Dendrograms distinguished the phytopathogenical isolate of Ceratorhiza sp. AGC from the mycorrhizal fungi studied. The symbionts of E. secundum were grouped into two clades, one containing Epulorhiza sp.1 isolates and the other the Epulorhiza sp.2 isolate. The similarity between the symbionts of E. denticulatum and Epulorhiza spp. fungi suggests that symbionts found in E. denticulatum may be identified as Epulorhiza. These results were corroborated by the analysis of the rDNA ITS region. The dendrogram constructed based on the Mahalanobis distance differentiated the clades most clearly. Fatty acid composition analysis proved to be a useful tool for characterizing and identifying Rhizoctonia-like mycorrhizal fungi.Sociedade Brasileira de Ciência do Solo2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400009Revista Brasileira de Ciência do Solo v.35 n.4 2011reponame:Revista Brasileira de Ciência do Solo (Online)instname:Sociedade Brasileira de Ciência do Solo (SBCS)instacron:SBCS10.1590/S0100-06832011000400009info:eu-repo/semantics/openAccessPereira,Marlon CorrêaVieira,Nívea MoreiraTótola,Marcos RogérioKasuya,Maria Catarina Megumieng2011-10-21T00:00:00Zoai:scielo:S0100-06832011000400009Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=0100-0683&lng=es&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcs@ufv.br1806-96570100-0683opendoar:2011-10-21T00:00Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS)false |
dc.title.none.fl_str_mv |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
title |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
spellingShingle |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. Pereira,Marlon Corrêa Rhizoctonia-like fungi Epidendrum spp. orchids FAME MIDI method ITS sequence analysis fungos rizoctonioides orquídeas Epidendrum spp FAME método MIDI análise da sequência da região ITS |
title_short |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
title_full |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
title_fullStr |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
title_full_unstemmed |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
title_sort |
Total fatty acid composition in the characterization and identification of orchid mycorrhizal fungi Epulorhiza spp. |
author |
Pereira,Marlon Corrêa |
author_facet |
Pereira,Marlon Corrêa Vieira,Nívea Moreira Tótola,Marcos Rogério Kasuya,Maria Catarina Megumi |
author_role |
author |
author2 |
Vieira,Nívea Moreira Tótola,Marcos Rogério Kasuya,Maria Catarina Megumi |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pereira,Marlon Corrêa Vieira,Nívea Moreira Tótola,Marcos Rogério Kasuya,Maria Catarina Megumi |
dc.subject.por.fl_str_mv |
Rhizoctonia-like fungi Epidendrum spp. orchids FAME MIDI method ITS sequence analysis fungos rizoctonioides orquídeas Epidendrum spp FAME método MIDI análise da sequência da região ITS |
topic |
Rhizoctonia-like fungi Epidendrum spp. orchids FAME MIDI method ITS sequence analysis fungos rizoctonioides orquídeas Epidendrum spp FAME método MIDI análise da sequência da região ITS |
description |
Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated from Epidendrum denticulatum, and a phytopathogenic fungus, Ceratorhiza sp. AGC, were grouped based on the profile of their fatty acids, which was assessed by the Euclidian and Mahalanobis distances and the UPGMA method. Dendrograms distinguished the phytopathogenical isolate of Ceratorhiza sp. AGC from the mycorrhizal fungi studied. The symbionts of E. secundum were grouped into two clades, one containing Epulorhiza sp.1 isolates and the other the Epulorhiza sp.2 isolate. The similarity between the symbionts of E. denticulatum and Epulorhiza spp. fungi suggests that symbionts found in E. denticulatum may be identified as Epulorhiza. These results were corroborated by the analysis of the rDNA ITS region. The dendrogram constructed based on the Mahalanobis distance differentiated the clades most clearly. Fatty acid composition analysis proved to be a useful tool for characterizing and identifying Rhizoctonia-like mycorrhizal fungi. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-06832011000400009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência do Solo |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência do Solo |
dc.source.none.fl_str_mv |
Revista Brasileira de Ciência do Solo v.35 n.4 2011 reponame:Revista Brasileira de Ciência do Solo (Online) instname:Sociedade Brasileira de Ciência do Solo (SBCS) instacron:SBCS |
instname_str |
Sociedade Brasileira de Ciência do Solo (SBCS) |
instacron_str |
SBCS |
institution |
SBCS |
reponame_str |
Revista Brasileira de Ciência do Solo (Online) |
collection |
Revista Brasileira de Ciência do Solo (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS) |
repository.mail.fl_str_mv |
||sbcs@ufv.br |
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1752126517116141568 |